Recombinant Human Langerin protein (His tag) is a Human Fragment protein, in the 65 to 328 aa range, expressed in Baculovirus, with >90% purity, < 1 EU/µg endotoxin level and suitable for SDS-PAGE.
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Key facts
- Purity
- >90% SDS-PAGE
- Endotoxin level
- < 1 EU/µg
- Expression system
- Baculovirus
- Tags
- His tag C-Terminus
- Applications
- SDS-PAGE
- Biologically active
- No
Amino acid sequence
A D P P R F M G T I S D V K T N V Q L L K G R V D N I S T L D S E I K K N S D G M E A A G V Q I Q M V N E S L G Y V R S Q F L K L K T S V E K A N A Q I Q I L T R S W E E V S T L N A Q I P E L K S D L E K A S A L N T K I R A L Q G S L E N M S K L L K R Q N D I L Q V V S Q G W K Y F K G N F Y Y F S L I P K T W Y S A E Q F C V S R N S H L T S V T S E S E Q E F L Y K T A G G L I Y W I G L T K A G M E G D W S W V D D T P F N K V Q S A R F W I P G E P N N A G N N E H C G N I K A P S L Q A W N D A P C D K T F L F I C K R P Y V P S E P H H H H H H
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
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Target data
Function
Calcium-dependent lectin displaying mannose-binding specificity. Induces the formation of Birbeck granules (BGs); is a potent regulator of membrane superimposition and zippering. Binds to sulfated as well as mannosylated glycans, keratan sulfate (KS) and beta-glucans. Facilitates uptake of antigens and is involved in the routing and/or processing of antigen for presentation to T cells. Major receptor on primary Langerhans cells for Candida species, Saccharomyces species, and Malassezia furfur. Protects against human immunodeficiency virus-1 (HIV-1) infection. Binds to high-mannose structures present on the envelope glycoprotein which is followed by subsequent targeting of the virus to the Birbeck granules leading to its rapid degradation.
Alternative names
CD207, CLEC4K, C-type lectin domain family 4 member K, Langerin
Recommended products
Recombinant Human Langerin protein (His tag) is a Human Fragment protein, in the 65 to 328 aa range, expressed in Baculovirus, with >90% purity, < 1 EU/µg endotoxin level and suitable for SDS-PAGE.
Key facts
- Purity
- >90% SDS-PAGE
- Endotoxin level
- < 1 EU/µg
- Expression system
- Baculovirus
- Tags
- His tag C-Terminus
- Applications
- SDS-PAGE
- Biologically active
- No
- Accession
- Q9UJ71-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 7.4
Constituents: 90% PBS, 10% Glycerol (glycerin, glycerine)
Sequence info
Amino acid sequence
- A D P P R F M G T I S D V K T N V Q L L K G R V D N I S T L D S E I K K N S D G M E A A G V Q I Q M V N E S L G Y V R S Q F L K L K T S V E K A N A Q I Q I L T R S W E E V S T L N A Q I P E L K S D L E K A S A L N T K I R A L Q G S L E N M S K L L K R Q N D I L Q V V S Q G W K Y F K G N F Y Y F S L I P K T W Y S A E Q F C V S R N S H L T S V T S E S E Q E F L Y K T A G G L I Y W I G L T K A G M E G D W S W V D D T P F N K V Q S A R F W I P G E P N N A G N N E H C G N I K A P S L Q A W N D A P C D K T F L F I C K R P Y V P S E P H H H H H H
- Accession
- Q9UJ71
- Protein length
- Fragment
- Predicted molecular weight
- 30.9 kDa
- Amino acids
- 65 to 328
- Nature
- Recombinant
- Tags
- His tag C-Terminus
Specifications
- Form
- Liquid
- Additional notes
Affinity purified
General info
- Function
Calcium-dependent lectin displaying mannose-binding specificity. Induces the formation of Birbeck granules (BGs); is a potent regulator of membrane superimposition and zippering. Binds to sulfated as well as mannosylated glycans, keratan sulfate (KS) and beta-glucans. Facilitates uptake of antigens and is involved in the routing and/or processing of antigen for presentation to T cells. Major receptor on primary Langerhans cells for Candida species, Saccharomyces species, and Malassezia furfur. Protects against human immunodeficiency virus-1 (HIV-1) infection. Binds to high-mannose structures present on the envelope glycoprotein which is followed by subsequent targeting of the virus to the Birbeck granules leading to its rapid degradation.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Langerin also known as CD207 is a type II transmembrane protein involved in the immune response. It weighs approximately 40 kDa and is expressed mainly in Langerhans cells a specialized subset of dendritic cells found in the skin and mucosal tissues. Langerin functions as a C-type lectin receptor with the ability to recognize and internalize glycosylated antigens. This target facilitates the capture and presentation of antigens enhancing the immune surveillance process.
- Biological function summary
Langerin supports Langerhans cells in the recognition and processing of pathogens. The protein forms homotrimers enabling it to bind specific mannose-rich glycan structures on pathogens such as viruses and bacteria. Through its endocytic properties Langerin internalizes these antigens into Birbeck granules unique organelles in Langerhans cells promoting antigen processing and presentation to T cells therefore initiating immune responses.
- Pathways
Langerin is critical for antigen uptake and processing via the lectin pathway of endocytosis. It interacts with other dendritic cell receptors and influences signaling pathways that enhance immune activation such as the Syk and Raf-1 kinase pathways. Langerin’s ability to internalize antigens connects it with proteins like DC-SIGN and MR which share roles in pathogen recognition and immune modulation.
- Associated diseases and disorders
Langerin plays a significant role in conditions like Langerhans Cell Histiocytosis (LCH) and HIV. In LCH abnormal proliferation of Langerhans cells occurs where Langerin expression helps in diagnosing and characterizing LCH lesions. In the context of HIV Langerin interacts with viral particles reducing their ability to infect immune cells. This protein also relates to other immune receptors like CD40 and CD86 which regulate immune responses in associated disorders.
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1 product image
SDS-PAGE - Recombinant Human Langerin protein (His tag) (ab219216)
15% SDS-PAGE analysis of ab219216 (3μg).
Molecular weight: 28-40KDa (SDS-PAGE under reducing conditions).
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