Recombinant Human LARS2 protein is a Human Full Length protein, in the 1 to 903 aa range, expressed in Wheat germ and suitable for ELISA, WB.
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Key facts
- Expression system
- Wheat germ
- Tags
- GST tag N-Terminus
- Applications
- ELISA, WB
- Biologically active
- No
Amino acid sequence
M A S V W Q R L G F Y A S L L K R Q L N G G P D V I K W E R R V I P G C T R S I Y S A T G K W T K E Y T L Q T R K D V E K W W H Q R I K E Q A S K I S E A D K S K P K F Y V L S M F P Y P S G K L H M G H V R V Y T I S D T I A R F Q K M R G M Q V I N P M G W D A F G L P A E N A A V E R N L H P Q S W T Q S N I K H M R K Q L D R L G L C F S W D R E I T T C L P D Y Y K W T Q Y L F I K L Y E A G L A Y Q K E A L V N W D P V D Q T V L A N E Q V D E H G C S W R S G A K V E Q K Y L R Q W F I K T T A Y A K A M Q D A L A D L P E W Y G I K G M Q A H W I G D C V G C H L D F T L K V H G Q A T G E K L T A Y T A T P E A I Y G T S H V A I S P S H R L L H G H S S L K E A L R M A L V P G K D C L T P V M A V N M L T Q Q E V P V V I L A K A D L E G S L D S K I G I P S T S S E D T I L A Q T L G L A Y S E V I E T L P D G T E R L S S S A E F T G M T R Q D A F L A L T Q K A R G K R V G G D V T S D K L K D W L I S R Q R Y W G T P I P I V H C P V C G P T P V P L E D L P V T L P N I A S F T G K G G P P L A M A S E W V N C S C P R C K G A A K R E T D T M D T F V D S A W Y Y F R Y T D P H N P H S P F N T A V A D Y W M P V D L Y I G G K E H A V M H L F Y A R F F S H F C H D Q K M V K H R E P F H K L L A Q G L I K G Q T F R L P S G Q Y L Q R E E V D L T G S V P V H A K T K E K L E V T W E K M S K S K H N G V D P E E V V E Q Y G I D T I R L Y I L F A A P P E K D I L W D V K T D A L P G V L R W Q Q R L W T L T T R F I E A R A S G K S P Q P Q L L S N K E K A E A R K L W E Y K N S V I S Q V T T H F T E D F S L N S A I S Q L M G L S N A L S Q A S Q S V I L H S P E F E D A L C A L M V M A A P L A P H V T S E I W A G L A L V P R K L C A H Y T W D A S V L L Q A W P A V D P E F L Q Q P E V V Q M A V L I N N K A C G K I P V P Q Q V A R D Q D K V H E F V L Q S E L G V R L L Q G R S I K K S F L S P R T A L I N F L V Q D
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application ELISA | Reactivity Reacts | Dilution info - | Notes - |
Application WB | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Catalyzes the attachment of leucine to its cognate tRNA.
Alternative names
KIAA0028, LARS2, Leucyl-tRNA synthetase, LeuRS
Recommended products
Recombinant Human LARS2 protein is a Human Full Length protein, in the 1 to 903 aa range, expressed in Wheat germ and suitable for ELISA, WB.
Key facts
- Expression system
- Wheat germ
- Tags
- GST tag N-Terminus
- Applications
- ELISA, WB
- Biologically active
- No
- Accession
- Q15031-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 8
Constituents: 0.79% Tris HCl, 0.31% Glutathione
Sequence info
Amino acid sequence
- M A S V W Q R L G F Y A S L L K R Q L N G G P D V I K W E R R V I P G C T R S I Y S A T G K W T K E Y T L Q T R K D V E K W W H Q R I K E Q A S K I S E A D K S K P K F Y V L S M F P Y P S G K L H M G H V R V Y T I S D T I A R F Q K M R G M Q V I N P M G W D A F G L P A E N A A V E R N L H P Q S W T Q S N I K H M R K Q L D R L G L C F S W D R E I T T C L P D Y Y K W T Q Y L F I K L Y E A G L A Y Q K E A L V N W D P V D Q T V L A N E Q V D E H G C S W R S G A K V E Q K Y L R Q W F I K T T A Y A K A M Q D A L A D L P E W Y G I K G M Q A H W I G D C V G C H L D F T L K V H G Q A T G E K L T A Y T A T P E A I Y G T S H V A I S P S H R L L H G H S S L K E A L R M A L V P G K D C L T P V M A V N M L T Q Q E V P V V I L A K A D L E G S L D S K I G I P S T S S E D T I L A Q T L G L A Y S E V I E T L P D G T E R L S S S A E F T G M T R Q D A F L A L T Q K A R G K R V G G D V T S D K L K D W L I S R Q R Y W G T P I P I V H C P V C G P T P V P L E D L P V T L P N I A S F T G K G G P P L A M A S E W V N C S C P R C K G A A K R E T D T M D T F V D S A W Y Y F R Y T D P H N P H S P F N T A V A D Y W M P V D L Y I G G K E H A V M H L F Y A R F F S H F C H D Q K M V K H R E P F H K L L A Q G L I K G Q T F R L P S G Q Y L Q R E E V D L T G S V P V H A K T K E K L E V T W E K M S K S K H N G V D P E E V V E Q Y G I D T I R L Y I L F A A P P E K D I L W D V K T D A L P G V L R W Q Q R L W T L T T R F I E A R A S G K S P Q P Q L L S N K E K A E A R K L W E Y K N S V I S Q V T T H F T E D F S L N S A I S Q L M G L S N A L S Q A S Q S V I L H S P E F E D A L C A L M V M A A P L A P H V T S E I W A G L A L V P R K L C A H Y T W D A S V L L Q A W P A V D P E F L Q Q P E V V Q M A V L I N N K A C G K I P V P Q Q V A R D Q D K V H E F V L Q S E L G V R L L Q G R S I K K S F L S P R T A L I N F L V Q D
- Accession
- Q15031
- Protein length
- Full Length
- Amino acids
- 1 to 903
- Nature
- Recombinant
- Tags
- GST tag N-Terminus
Specifications
- Form
- Liquid
General info
- Function
Catalyzes the attachment of leucine to its cognate tRNA.
- Sequence similarities
Belongs to the class-I aminoacyl-tRNA synthetase family.
- Subcellular localisation
- Mitochondrion matrix
Storage
- Shipped at conditions
- Dry Ice
- Appropriate short-term storage conditions
- -80°C
- Appropriate long-term storage conditions
- -80°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
LARS2 also known as mitochondrial leucyl-tRNA synthetase 2 plays a significant role in protein biosynthesis. This enzyme is part of the class II aminoacyl-tRNA synthetase family with a molecular mass of around 108 kDa. LARS2 primarily functions in mitochondria ensuring the proper attachment of leucine to its corresponding tRNA an essential step for protein translation. Expression of LARS2 occurs most notably in tissues with high metabolic demands such as muscle and liver where mitochondrial activity is important.
- Biological function summary
LARS2 facilitates the translation of mitochondrial-encoded proteins by charging tRNA with leucine. It operates as a monomer and does not participate in a larger complex. This charging process is essential for maintaining mitochondrial function and energy production ensuring the correct synthesis of respiratory chain components. LARS2 thereby provides support for the translation machinery within mitochondria.
- Pathways
LARS2 plays an indispensable role within the mitochondrial translation system. It directly influences the oxidative phosphorylation pathway by enabling the synthesis of components important for electron transport and ATP production. LARS2 interacts functionally with other mitochondrial tRNA synthetases like GARS2 and YARS2 contributing to the efficient translation of proteins fundamental for cellular respiration.
- Associated diseases and disorders
Mutations in the LARS2 gene have been linked to Perrault syndrome a condition characterized by ovarian dysgenesis and sensorineural deafness. Additionally disruptions in LARS2 activity may also contribute to mitochondrial encephalopathy. The dysfunction of LARS2 affects proteins within the mitochondrial oxidative phosphorylation pathway impacting ATP production and cellular energy balance which further associates with disorders involving METTL1 and other mitochondrial synthetases impacting similar processes.
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1 product image
SDS-PAGE - Recombinant Human LARS2 protein (ab161621)
ab161621 on a 12.5% SDS-PAGE stained with Coomassie Blue.
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