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Recombinant Human LC3B protein is a Human Full Length protein, in the 1 to 140 aa range, expressed in Escherichia coli, with >95% purity and suitable for SDS-PAGE, MS.
Alternative names=Microtubule-associated proteins 1A/1B light chain 3B, Autophagy-related protein LC3 B, Autophagy-related ubiquitin-like modifier LC3 B, MAP1 light chain 3-like protein 2, MAP1A/MAP1B light chain 3 B, Microtubule-associated protein 1 light chain 3 beta, MAP1A/MAP1B LC3 B, MAP1ALC3, MAP1LC3B
- Purity
>95% SDS-PAGE
- Expression system
Escherichia coli
- Tags
His tag N-Terminus
- Applications
SDS-PAGE, MS
- Biologically active
No
- Sequence
M G S S H H H H H H S S G L V P R G S H M P S E K T F K Q R R T F E Q R V E D V R L I R E Q H P T K I P V I I E R Y K G E K Q L P V L D K T K F L V P D H V N M S E L I K I I R R R L Q L N A N Q A F F L L V N G H S M V S V S T P I S E V Y E S E K D E D G F L Y
M V Y A S Q E T F G
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application MS | Reactivity Reacts | Dilution info - | Notes - |
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Recombinant Human LC3B protein is a Human Full Length protein, in the 1 to 140 aa range, expressed in Escherichia coli, with >95% purity and suitable for SDS-PAGE, MS.
Alternative names=Microtubule-associated proteins 1A/1B light chain 3B, Autophagy-related protein LC3 B, Autophagy-related ubiquitin-like modifier LC3 B, MAP1 light chain 3-like protein 2, MAP1A/MAP1B light chain 3 B, Microtubule-associated protein 1 light chain 3 beta, MAP1A/MAP1B LC3 B, MAP1ALC3, MAP1LC3B
Key facts
- Purity
>95% SDS-PAGE
- Expression system
Escherichia coli
- Tags
His tag N-Terminus
- Applications
SDS-PAGE, MS
- Biologically active
No
- Predicted molecular weight
16.2 kDa
- Amino acids
1 to 140
- Sequence
M G S S H H H H H H S S G L V P R G S H M P S E K T F K Q R R T F E Q R V E D V R L I R E Q H P T K I P V I I E R Y K G E K Q L P V L D K T K F L V P D H V N M S E L I K I I R R R L Q L N A N Q A F F L L V N G H S M V S V S T P I S E V Y E S E K D E D G F L Y
M V Y A S Q E T F G- Accession
- Q9GZQ8-1
- Protein length
Full Length
- Animal free
No
- Nature
Recombinant
- Species
Human
- Concentration
- Storage buffer
pH: 8
Constituents: 20% Glycerol (glycerin, glycerine), 0.58% Sodium chloride, 0.316% Tris HCl, 0.0154% (R*,R*)-1,4-Dimercaptobutan-2,3-diol
Specifications
- Form
Liquid
General info
- Function
Ubiquitin-like modifier involved in formation of autophagosomal vacuoles (autophagosomes). Plays a role in mitophagy which contributes to regulate mitochondrial quantity and quality by eliminating the mitochondria to a basal level to fulfill cellular energy requirements and preventing excess ROS production (PubMed:28017329). While LC3s are involved in elongation of the phagophore membrane, the GABARAP/GATE-16 subfamily is essential for a later stage in autophagosome maturation. Promotes primary ciliogenesis by removing OFD1 from centriolar satellites via the autophagic pathway. Through its interaction with the reticulophagy receptor TEX264, participates in the remodeling of subdomains of the endoplasmic reticulum into autophagosomes upon nutrient stress, which then fuse with lysosomes for endoplasmic reticulum turnover (PubMed:31006538, PubMed:31006537).
- Sequence similarities
Belongs to the ATG8 family.
- Post-translational modifications
The precursor molecule is cleaved by ATG4B to form the cytosolic form, LC3-I. This is activated by APG7L/ATG7, transferred to ATG3 and conjugated to phospholipid to form the membrane-bound form, LC3-II (PubMed:15187094).
- Subcellular localisation
Cytoskeleton
Storage
- Shipped at conditions
Blue Ice
- Appropriate long-term storage conditions
-20°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Avoid freeze / thaw cycle
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