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AB123175

Recombinant Human LCMT1 protein (His tag N-Terminus)

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Recombinant Human LCMT1 protein (His tag N-Terminus) is a Human Full Length protein, in the 1 to 334 aa range, expressed in Escherichia coli, with >95%, suitable for SDS-PAGE, Mass Spec.

View Alternative Names

LCMT, CGI-68, LCMT1, Leucine carboxyl methyltransferase 1, Protein-leucine O-methyltransferase, [Phosphatase 2A protein]-leucine-carboxy methyltransferase 1

1 Images
SDS-PAGE - Recombinant Human LCMT1 protein (His tag N-Terminus) (AB123175)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant Human LCMT1 protein (His tag N-Terminus) (AB123175)

SDS-PAGE of ab123175 (3µg) under reducing condition and visualized by coomassie blue stain.

Key facts

Purity

>95% SDS-PAGE

Expression system

Escherichia coli

Tags

His tag N-Terminus

Applications

Mass Spec, SDS-PAGE

applications

Biologically active

No

Accession

Q9UIC8

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 8 Constituents: Tris HCl, 10% Glycerol (glycerin, glycerine), 0.88% Sodium chloride, 0.02% (R*,R*)-1,4-Dimercaptobutan-2,3-diol

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "Mass Spec": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Sequence info

[{"sequence":"MGSSHHHHHHSSGLVPRGSHMGSHMATRQRESSITSCCSTSSCDADDEGVRGTCEDASLCKRFAVSIGYWHDPYIQHFVRLSKERKAPEINRGYFARVHGVSQLIKAFLRKTECHCQIVNLGAGMDTTFWRLKDEDLLPSKYFEVDFPMIVTRKLHSIKCKPPLSSPILELHSEDTLQMDGHILDSKRYAVIGADLRDLSELEEKLKKCNMNTQLPTLLIAECVLVYMTPEQSANLLKWAANSFERAMFINYEQVNMGDRFGQIMIENLRRRQCDLAGVETCKSLESQKERLLSNGWETASAVDMMELYNRLPRAEVSRIESLEFLDEMELLEQLMRHYCLCWATKGGNELGLKEITY","proteinLength":"Full Length","predictedMolecularWeight":"41 kDa","actualMolecularWeight":null,"aminoAcidEnd":334,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"Q9UIC8","tags":[{"tag":"His","terminus":"N-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

LCMT1 also known as leucine carboxyl methyltransferase 1 is an enzyme with a mass of approximately 25 kDa. This enzyme plays an important role in the methylation of the C-terminal leucine residues of proteins specifically targeting protein phosphatase 2A (PP2A). It methylates the PP2A catalytic subunit affecting its activity and interactions. LCMT1 expression occurs widely in tissues with high expression noted in the brain and testis.
Biological function summary

This enzyme contributes to the dynamic regulation of protein phosphatase activity within cells. LCMT1 operates as part of a complex with PP2A and influences the formation of PP2A holoenzymes. By methylating the catalytic subunit of PP2A LCMT1 impacts the holoenzyme's substrate specificity and enzymatic activity contributing to cellular signaling and homeostasis.

Pathways

LCMT1 activity integrates into the regulation of various signaling cascades notably the MAPK and AKT pathways. These pathways are important for cell growth survival and differentiation. LCMT1 influences these pathways by modulating PP2A activity which can dephosphorylate key signaling molecules such as ERK and AKT. LCMT1 works in concert with other proteins like PME-1 that demethylate PP2A to fine-tune this regulation.

LCMT1 has connections to neurodegenerative diseases and certain cancers. In neurodegenerative diseases such as Alzheimer's disease altered LCMT1 activity can lead to dysregulation of PP2A which affects tau phosphorylation and accumulation. Moreover in cancer such as hepatocellular carcinoma LCMT1's regulation of PP2A can influence cellular proliferation and apoptosis. It interacts functionally with proteins like alpha4 which is associated with PP2A and involved in their pathogenesis.
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Specifications

Form

Liquid

Additional notes

ab123175 is purified by using conventional chromatography techniques.

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General info

Function

Methylates the carboxyl group of the C-terminal leucine residue of protein phosphatase 2A catalytic subunits to form alpha-leucine ester residues.

Sequence similarities

Belongs to the methyltransferase superfamily. LCMT family.

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Product protocols

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Target data

Methylates the carboxyl group of the C-terminal leucine residue of protein phosphatase 2A catalytic subunits to form alpha-leucine ester residues.
See full target information LCMT1
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Complementary Products

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AB169337

Anti-LCMT1 antibody

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Primary Antibodies

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Primary Antibodies

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Anti-CK1 epsilon antibody [1/Casein Kinase Iε]

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Immunocytochemistry/ Immunofluorescence - Anti-CK1 epsilon antibody [1/Casein Kinase Iε] (AB302638)

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Primary Antibodies

AB155996

Anti-PDHB antibody [EPR11097(B)]

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDHB antibody [EPR11097(B)] (AB155996)

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