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AB198657

Recombinant human LDL Receptor protein (Biotin) (DDDDK tag C-Terminus + Avi tag C-Terminus)

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(1 Publication)

Recombinant human LDL Receptor protein (Biotin) (DDDDK tag C-Terminus + Avi tag C-Terminus) is a Human Fragment protein, in the 22 to 788 aa range, expressed in HEK 293 cells, with >95%, suitable for SDS-PAGE, FuncS, WB.

View Alternative Names

Low-density lipoprotein receptor, LDL receptor, LDLR

2 Images
Functional Studies - Recombinant human LDL Receptor protein (Biotin) (DDDDK tag C-Terminus + Avi tag C-Terminus) (AB198657)
  • FuncS

Supplier Data

Functional Studies - Recombinant human LDL Receptor protein (Biotin) (DDDDK tag C-Terminus + Avi tag C-Terminus) (AB198657)

Example of specific activity of ab198657

SDS-PAGE - Recombinant human LDL Receptor protein (Biotin) (DDDDK tag C-Terminus + Avi tag C-Terminus) (AB198657)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant human LDL Receptor protein (Biotin) (DDDDK tag C-Terminus + Avi tag C-Terminus) (AB198657)

4-20% SDS Page analysis of ab198657 (2 μg). The two bands shown correspond to differing states of glycosylation.

Key facts

Purity

>95% SDS-PAGE

Expression system

HEK 293 cells

Tags

DDDDK tag C-Terminus Avi tag C-Terminus

Applications

SDS-PAGE, WB, FuncS

applications

Biologically active

Yes

Biological activity

Assay Conditions: Add a 50 μL reaction mix (50 mM HEPES pH 7.4, 50 mM NaCl, 200 μM CaCl2, 0.05% Tween-20, 0.1% BSA, and up to 1500 ng PCSK9) to the wells of a streptavidin plate precoated with ab198657 and incubate for 2 hr. Add 6xHis antibody and incubate 1 hr. Add secondary HRP labeled antibody and incubate 30 min. Finally, add HRP chemiluminescent substrates and read luminescence.

Conjugation

Biotin

Excitation/Emission
Accession

P01130

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 8 Constituents: 20% Glycerol (glycerin, glycerine), 0.64% Sodium chloride, 0.63% Tris HCl, 0.02% Potassium chloride

storage-buffer

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Complementary Products

Primary Antibodies

AB30682

Anti-SREBP2 antibody

Western blot - Anti-SREBP2 antibody (AB30682)

  • 4
  • 12
Primary Antibodies

AB52818

Anti-LDL Receptor antibody [EP1553Y]

RabMAb|KO Validated|Recombinant

Western blot - Anti-LDL Receptor antibody [EP1553Y] (AB52818)

  • 4
  • 16
Reagents, Controls & Accessories

AB203359

Pre-coated 384 well Microplate SimpleStep ELISA®

SimpleStep

ELISA - Pre-coated 384 well Microplate SimpleStep ELISA® (AB203359)

  • 5
  • 1
Assay Kits

AB233621

Mouse GFAP ELISA Kit

Recombinant|SimpleStep

ELISA - Mouse GFAP ELISA Kit (AB233621)

  • 0
  • 0
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "FuncS": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Sequence info

[{"sequence":"AVGDRCERNEFQCQDGKCISYKWVCDGSAECQDGSDESQETCLSVTCKSGDFSCGGRVNRCIPQFWRCDGQVDCDNGSDEQGCPPKTCSQDEFRCHDGKCISRQFVCDSDRDCLDGSDEASCPVLTCGPASFQCNSSTCIPQLWACDNDPDCEDGSDEWPQRCRGLYVFQGDSSPCSAFEFHCLSGECIHSSWRCDGGPDCKDKSDEENCAVATCRPDEFQCSDGNCIHGSRQCDREYDCKDMSDEVGCVNVTLCEGPNKFKCHSGECITLDKVCNMARDCRDWSDEPIKECGTNECLDNNGGCSHVCNDLKIGYECLCPDGFQLVAQRRCEDIDECQDPDTCSQLCVNLEGGYKCQCEEGFQLDPHTKACKAVGSIAYLFFTNRHEVRKMTLDRSEYTSLIPNLRNVVALDTEVASNRIYWSDLSQRMICSTQLDRAHGVSSYDTVISRDIQAPDGLAVDWIHSNIYWTDSVLGTVSVADTKGVKRKTLFRENGSKPRAIVVDPVHGFMYWTDWGTPAKIKKGGLNGVDIYSLVTENIQWPNGITLDLLSGRLYWVDSKLHSISSIDVNGGNRKTILEDEKRLAHPFSLAVFEDKVFWTDIINEAIFSANRLTGSDVNLLAENLLSPEDMVLFHNLTQPRGVNWCERTTLSNGGCQYLCLPAPQINPHSPKFTCACPDGMLLARDMRSCLTEAEAAVATQETSTVRLKVSSTAVRTQHTTTRPVPDTSRLPGATPGLTTVEIVTMSHQALGDVAGRGNEKKPSSVRDYKDDDDKGGGLNDIFEAQKIEWHE","proteinLength":"Fragment","predictedMolecularWeight":"87.7 kDa","actualMolecularWeight":null,"aminoAcidEnd":788,"aminoAcidStart":22,"nature":"Recombinant","expressionSystem":"HEK 293 cells","accessionNumber":"P01130","tags":[{"tag":"DDDDK","terminus":"C-Terminus"},{"tag":"Avi","terminus":"C-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Dry Ice
Appropriate short-term storage conditions
-80°C
Appropriate long-term storage conditions
-80°C
Storage information
Avoid freeze / thaw cycle
True
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The LDL Receptor also called LDLR is a protein that plays an important role in the uptake and clearance of low-density lipoproteins (LDL) from the bloodstream. LDLR binds LDL particles facilitating their internalization through receptor-mediated endocytosis. This receptor is expressed mainly in the liver adrenal glands and other tissues involved in cholesterol metabolism. It has an approximate molecular weight of 160 kDa. Researchers often study LDLR using techniques like Western blotting to understand its presence and function.
Biological function summary

The LDL receptor interacts with LDL particles to regulate cholesterol levels in the body. It is part of a cell surface complex that recognizes and binds to apolipoprotein B-100 or apolipoprotein E present on LDL. This interaction initiates internalization of LDL leading to its degradation in lysosomes where cholesterol can be released and used by the cell. Mutations in the gene encoding LDLR can lead to inefficient cholesterol uptake influencing various metabolic processes.

Pathways

LDL receptor activities are integral to lipid metabolism and cholesterol homeostasis. Two important biological pathways that involve LDLR include the cholesterol biosynthesis pathway and the lipoprotein clearance pathway. Within these pathways LDLR collaborates closely with proteins like PCSK9 which modulates its expression and degradation and HMG-CoA reductase an important enzyme in cholesterol synthesis to balance cholesterol levels in the body.

Defects in the LDL receptor are strongly associated with familial hypercholesterolemia and atherosclerosis. These conditions arise from impaired clearance of LDL leading to elevated cholesterol levels which pose risks for cardiovascular diseases. LDLR dysfunctions are linked with the Protein PCSK9 whose gain-of-function mutations can exacerbate hypercholesterolemia by promoting degradation of LDLR while statins aim to increase LDLR expression to lower LDL cholesterol levels.
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Specifications

Form

Liquid

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General info

Function

Binds low density lipoprotein /LDL, the major cholesterol-carrying lipoprotein of plasma, and transports it into cells by endocytosis. In order to be internalized, the receptor-ligand complexes must first cluster into clathrin-coated pits. Forms a ternary complex with PGRMC1 and TMEM97 receptors which increases LDLR-mediated LDL internalization (PubMed : 30443021).. (Microbial infection) Acts as a receptor for hepatitis C virus in hepatocytes, but not through a direct interaction with viral proteins.. (Microbial infection) Acts as a receptor for Vesicular stomatitis virus.. (Microbial infection) In case of HIV-1 infection, may function as a receptor for extracellular Tat in neurons, mediating its internalization in uninfected cells.. (Microbial infection) Acts as a receptor for Crimean-Congo hemorrhagic fever virus (CCHFV).. (Microbial infection) Acts as a receptor for many Alphavirus, including Getah virus (GETV), Ross river virus (RRV) and Semliki Forest virus.

Sequence similarities

Belongs to the LDLR family.

Post-translational modifications

N- and O-glycosylated.. Ubiquitinated by MYLIP leading to degradation.

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Product protocols

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Target data

Binds low density lipoprotein /LDL, the major cholesterol-carrying lipoprotein of plasma, and transports it into cells by endocytosis. In order to be internalized, the receptor-ligand complexes must first cluster into clathrin-coated pits. Forms a ternary complex with PGRMC1 and TMEM97 receptors which increases LDLR-mediated LDL internalization (PubMed : 30443021).. (Microbial infection) Acts as a receptor for hepatitis C virus in hepatocytes, but not through a direct interaction with viral proteins.. (Microbial infection) Acts as a receptor for Vesicular stomatitis virus.. (Microbial infection) In case of HIV-1 infection, may function as a receptor for extracellular Tat in neurons, mediating its internalization in uninfected cells.. (Microbial infection) Acts as a receptor for Crimean-Congo hemorrhagic fever virus (CCHFV).. (Microbial infection) Acts as a receptor for many Alphavirus, including Getah virus (GETV), Ross river virus (RRV) and Semliki Forest virus.
See full target information Low-density lipoprotein receptor
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

CNS neuroscience & therapeutics 31:e70413 PubMed40384564

2025

Kisspeptin-10 Prevents the Development of Cerebral Aneurysms by Reducing the Expression of Egr-1.

Applications

Unspecified application

Species

Unspecified reactive species

Huimin Yu,Minghong Xie,Xuancong Liufu,Yezi Xu,Lei Chen
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