Recombinant Human LEO1/RDL protein is a Human Full Length protein, in the 1 to 666 aa range, expressed in Wheat germ and suitable for SDS-PAGE, ELISA, WB.
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Key facts
- Expression system
- Wheat germ
- Tags
- Tag free
- Applications
- SDS-PAGE, ELISA, WB
- Biologically active
- No
Amino acid sequence
M A D M E D L F G S D A D S E A E R K D S D S G S D S D S D Q E N A A S G S N A S G S E S D Q D E R G D S G Q P S N K E L F G D D S E D E G A S H H S G S D N H S E R S D N R S E A S E R S D H E D N D P S D V D Q H S G S E A P N D D E D E G H R S D G G S H H S E A E G S E K A H S D D E K W G R E D K S D Q S D D E K I Q N S D D E E R A Q G S D E D K L Q N S D D D E K M Q N T D D E E R P Q L S D D E R Q Q L S E E E K A N S D D E R P V A S D N D D E K Q N S D D E E Q P Q L S D E E K M Q N S D D E R P Q A S D E E H R H S D D E E E Q D H K S E S A R G S D S E D E V L R M K R K N A I A S D S E A D S D T E V P K D N S G T M D L F G G A D D I S S G S D G E D K P P T P G Q P V D E N G L P Q D Q Q E E E P I P E T R I E V E I P K V N T D L G N D L Y F V K L P N F L S V E P R P F D P Q Y Y E D E F E D E E M L D E E G R T R L K L K V E N T I R W R I R R D E E G N E I K E S N A R I V K W S D G S M S L H L G N E V F D V Y K A P L Q G D H N H L F I R Q G T G L Q G Q A V F K T K L T F R P H S T D S A T H R K M T L S L A D R C S K T Q K I R I L P M A G R D P E C Q R T E M I K K E E E R L R A S I R R E S Q Q R R M R E K Q H Q R G L S A S Y L E P D R Y D E E E E G E E S I S L A A I K N R Y K G G I R E E R A R I Y S S D S D E G S E E D K A Q R L L K A K K L T S D E E G E P S G K R K A E D D D K A N K K H K K Y V I S D E E E E D D D
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application ELISA | Reactivity Reacts | Dilution info - | Notes - |
Application WB | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Component of the PAF1 complex (PAF1C) which has multiple functions during transcription by RNA polymerase II and is implicated in regulation of development and maintenance of embryonic stem cell pluripotency. PAF1C associates with RNA polymerase II through interaction with POLR2A CTD non-phosphorylated and 'Ser-2'- and 'Ser-5'-phosphorylated forms and is involved in transcriptional elongation, acting both independently and synergistically with TCEA1 and in cooperation with the DSIF complex and HTATSF1. PAF1C is required for transcription of Hox and Wnt target genes. PAF1C is involved in hematopoiesis and stimulates transcriptional activity of KMT2A/MLL1; it promotes leukemogenesis through association with KMT2A/MLL1-rearranged oncoproteins, such as KMT2A/MLL1-MLLT3/AF9 and KMT2A/MLL1-MLLT1/ENL. PAF1C is involved in histone modifications such as ubiquitination of histone H2B and methylation on histone H3 'Lys-4' (H3K4me3). PAF1C recruits the RNF20/40 E3 ubiquitin-protein ligase complex and the E2 enzyme UBE2A or UBE2B to chromatin which mediate monoubiquitination of 'Lys-120' of histone H2B (H2BK120ub1); UB2A/B-mediated H2B ubiquitination is proposed to be coupled to transcription. PAF1C is involved in mRNA 3' end formation probably through association with cleavage and poly(A) factors. In case of infection by influenza A strain H3N2, PAF1C associates with viral NS1 protein, thereby regulating gene transcription. Involved in polyadenylation of mRNA precursors. Connects PAF1C to Wnt signaling.
Alternative names
RDL, LEO1, RNA polymerase-associated protein LEO1, Replicative senescence down-regulated leo1-like protein
Recommended products
Recombinant Human LEO1/RDL protein is a Human Full Length protein, in the 1 to 666 aa range, expressed in Wheat germ and suitable for SDS-PAGE, ELISA, WB.
Key facts
- Expression system
- Wheat germ
- Tags
- Tag free
- Applications
- SDS-PAGE, ELISA, WB
- Biologically active
- No
- Accession
- Q8WVC0-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 8
Constituents: 0.79% Tris HCl, 0.31% Glutathione
Sequence info
Amino acid sequence
- M A D M E D L F G S D A D S E A E R K D S D S G S D S D S D Q E N A A S G S N A S G S E S D Q D E R G D S G Q P S N K E L F G D D S E D E G A S H H S G S D N H S E R S D N R S E A S E R S D H E D N D P S D V D Q H S G S E A P N D D E D E G H R S D G G S H H S E A E G S E K A H S D D E K W G R E D K S D Q S D D E K I Q N S D D E E R A Q G S D E D K L Q N S D D D E K M Q N T D D E E R P Q L S D D E R Q Q L S E E E K A N S D D E R P V A S D N D D E K Q N S D D E E Q P Q L S D E E K M Q N S D D E R P Q A S D E E H R H S D D E E E Q D H K S E S A R G S D S E D E V L R M K R K N A I A S D S E A D S D T E V P K D N S G T M D L F G G A D D I S S G S D G E D K P P T P G Q P V D E N G L P Q D Q Q E E E P I P E T R I E V E I P K V N T D L G N D L Y F V K L P N F L S V E P R P F D P Q Y Y E D E F E D E E M L D E E G R T R L K L K V E N T I R W R I R R D E E G N E I K E S N A R I V K W S D G S M S L H L G N E V F D V Y K A P L Q G D H N H L F I R Q G T G L Q G Q A V F K T K L T F R P H S T D S A T H R K M T L S L A D R C S K T Q K I R I L P M A G R D P E C Q R T E M I K K E E E R L R A S I R R E S Q Q R R M R E K Q H Q R G L S A S Y L E P D R Y D E E E E G E E S I S L A A I K N R Y K G G I R E E R A R I Y S S D S D E G S E E D K A Q R L L K A K K L T S D E E G E P S G K R K A E D D D K A N K K H K K Y V I S D E E E E D D D
- Accession
- Q8WVC0
- Protein length
- Full Length
- Predicted molecular weight
- 101.8 kDa
- Amino acids
- 1 to 666
- Nature
- Recombinant
Specifications
- Form
- Liquid
General info
- Function
Component of the PAF1 complex (PAF1C) which has multiple functions during transcription by RNA polymerase II and is implicated in regulation of development and maintenance of embryonic stem cell pluripotency. PAF1C associates with RNA polymerase II through interaction with POLR2A CTD non-phosphorylated and 'Ser-2'- and 'Ser-5'-phosphorylated forms and is involved in transcriptional elongation, acting both independently and synergistically with TCEA1 and in cooperation with the DSIF complex and HTATSF1. PAF1C is required for transcription of Hox and Wnt target genes. PAF1C is involved in hematopoiesis and stimulates transcriptional activity of KMT2A/MLL1; it promotes leukemogenesis through association with KMT2A/MLL1-rearranged oncoproteins, such as KMT2A/MLL1-MLLT3/AF9 and KMT2A/MLL1-MLLT1/ENL. PAF1C is involved in histone modifications such as ubiquitination of histone H2B and methylation on histone H3 'Lys-4' (H3K4me3). PAF1C recruits the RNF20/40 E3 ubiquitin-protein ligase complex and the E2 enzyme UBE2A or UBE2B to chromatin which mediate monoubiquitination of 'Lys-120' of histone H2B (H2BK120ub1); UB2A/B-mediated H2B ubiquitination is proposed to be coupled to transcription. PAF1C is involved in mRNA 3' end formation probably through association with cleavage and poly(A) factors. In case of infection by influenza A strain H3N2, PAF1C associates with viral NS1 protein, thereby regulating gene transcription. Involved in polyadenylation of mRNA precursors. Connects PAF1C to Wnt signaling.
- Sequence similarities
Belongs to the LEO1 family.
- Subcellular localisation
- Nucleus
Storage
- Shipped at conditions
- Dry Ice
- Appropriate short-term storage conditions
- -80°C
- Appropriate long-term storage conditions
- -80°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
This product was previously labelled as LEO1.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
LEO1 also known as RDL is a subunit of the polymerase-associated factor 1 complex (Paf1C). It has a mass of approximately 64 kDa. LEO1 is expressed in many tissues with higher expression levels observed in the brain heart and skeletal muscle. This protein plays a role in the regulation of gene transcription by associating with RNA polymerase II. LEO1 is conserved across different species highlighting its importance in various biological processes.
- Biological function summary
LEO1/RDL contributes significantly to the transcription elongation process. As part of the larger Paf1 complex it interacts with other subunits to mediate histone modification influencing chromatin structure and gene expression. LEO1 regulates the transcription of various genes including those involved in cell growth and differentiation. Its presence is essential for the proper assembly and function of the Paf1 complex affecting the stability and activity of its associated proteins.
- Pathways
LEO1/RDL plays a pivotal role in the regulation of the Wnt signaling pathway and RNA polymerase II transcription pathway. In the Wnt pathway LEO1 interacts with key proteins to influence gene transcription patterns necessary for cell fate determination and embryonic development. It also closely associates with Cdc73 and Paf1 proteins within its complex coordinating transcriptional regulation and chromatin remodeling steps ensuring accuracy and efficiency in gene expression processes.
- Associated diseases and disorders
Alterations in LEO1/RDL expression or function have been linked to cancer and developmental disorders. The aberrant regulation of LEO1 can disrupt normal transcriptional processes leading to unchecked cell proliferation and tumorigenesis. LEO1's interaction with Cdc73 a protein associated with the Hyperparathyroidism-jaw tumor syndrome can affect the manifestation of this disorder and related pathologies. Understanding the role of LEO1 in these contexts aids in developing targeted therapeutic approaches.
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1 product image
Western blot - Recombinant Human LEO1/RDL protein (ab132311)
ab132311 on 12.5% SDS-PAGE stained with Coomassie blue.
All lanes: Western blot - Recombinant Human LEO1/RDL protein (ab132311)
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