Recombinant Human Leukotriene A4 hydrolase/LTA4H protein is a Human Full Length protein, in the 1 to 611 aa range, expressed in Escherichia coli, with >90% purity and suitable for SDS-PAGE.
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Key facts
- Purity
- >90% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- DDDDK tag C-Terminus His tag C-Terminus
- Applications
- SDS-PAGE
- Biologically active
- No
Amino acid sequence
M P E I V D T C S L A S P A S V C R T K H L H L R C S V D F T R R T L T G T A A L T V Q S Q E D N L R S L V L D T K D L T I E K V V I N G Q E V K Y A L G E R Q S Y K G S P M E I S L P I A L S K N Q E I V I E I S F E T S P K S S A L Q W L T P E Q T S G K E H P Y L F S Q C Q A I H C R A I L P C Q D T P S V K L T Y T A E V S V P K E L V A L M S A I R D G E T P D P E D P S R K I Y K F I Q K V P I P C Y L I A L V V G A L E S R Q I G P R T L V W S E K E Q V E K S A Y E F S E T E S M L K I A E D L G G P Y V W G Q Y D L L V L P P S F P Y G G M E N P C L T F V T P T L L A G D K S L S N V I A H E I S H S W T G N L V T N K T W D H F W L N E G H T V Y L E R H I C G R L F G E K F R H F N A L G G W G E L Q N S V K T F G E T H P F T K L V V D L T D I D P D V A Y S S V P Y E K G F A L L F Y L E Q L L G G P E I F L G F L K A Y V E K F S Y K S I T T D D W K D F L Y S Y F K D K V D V L N Q V D W N A W L Y S P G L P P I K P N Y D M T L T N A C I A L S Q R W I T A K E D D L N S F N A T D L K D L S S H Q L N E F L A Q T L Q R A P L P L G H I K R M Q E V Y N F N A I N N S E I R F R W L R L C I Q S K W E D A I P L A L K M A T E Q G R M K F T R P L F K D L A A F D K S H D Q A V R T Y Q E H K A S M H P V T A M L V G K D L K V D D Y K D D D D K H H H H H H
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Associated Products
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Target data
Function
Bifunctional zinc metalloenzyme that comprises both epoxide hydrolase (EH) and aminopeptidase activities. Acts as an epoxide hydrolase to catalyze the conversion of LTA4 to the pro-inflammatory mediator leukotriene B4 (LTB4) (PubMed:11917124, PubMed:12207002, PubMed:15078870, PubMed:18804029, PubMed:1897988, PubMed:1975494, PubMed:2244921). Has also aminopeptidase activity, with high affinity for N-terminal arginines of various synthetic tripeptides (PubMed:18804029, PubMed:20813919). In addition to its pro-inflammatory EH activity, may also counteract inflammation by its aminopeptidase activity, which inactivates by cleavage another neutrophil attractant, the tripeptide Pro-Gly-Pro (PGP), a bioactive fragment of collagen generated by the action of matrix metalloproteinase-9 (MMP9) and prolylendopeptidase (PREPL) (PubMed:20813919, PubMed:24591641). Involved also in the biosynthesis of resolvin E1 and 18S-resolvin E1 from eicosapentaenoic acid, two lipid mediators that show potent anti-inflammatory and pro-resolving actions (PubMed:21206090).
Alternative names
LTA4, LTA4H, Leukotriene A-4 hydrolase, LTA-4 hydrolase, Leukotriene A(4) hydrolase, Tripeptide aminopeptidase LTA4H
Recommended products
Recombinant Human Leukotriene A4 hydrolase/LTA4H protein is a Human Full Length protein, in the 1 to 611 aa range, expressed in Escherichia coli, with >90% purity and suitable for SDS-PAGE.
Key facts
- Purity
- >90% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- DDDDK tag C-Terminus His tag C-Terminus
- Applications
- SDS-PAGE
- Biologically active
- No
- Accession
- P09960-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 8
Preservative: 0.68% Imidazole
Constituents: 20% Glycerol (glycerin, glycerine), 0.64% Sodium chloride, 0.64% Tris HCl, 0.04% Sorbitan monolaurate, ethoxylated, 0.017% Potassium chloride
Sequence info
Amino acid sequence
- M P E I V D T C S L A S P A S V C R T K H L H L R C S V D F T R R T L T G T A A L T V Q S Q E D N L R S L V L D T K D L T I E K V V I N G Q E V K Y A L G E R Q S Y K G S P M E I S L P I A L S K N Q E I V I E I S F E T S P K S S A L Q W L T P E Q T S G K E H P Y L F S Q C Q A I H C R A I L P C Q D T P S V K L T Y T A E V S V P K E L V A L M S A I R D G E T P D P E D P S R K I Y K F I Q K V P I P C Y L I A L V V G A L E S R Q I G P R T L V W S E K E Q V E K S A Y E F S E T E S M L K I A E D L G G P Y V W G Q Y D L L V L P P S F P Y G G M E N P C L T F V T P T L L A G D K S L S N V I A H E I S H S W T G N L V T N K T W D H F W L N E G H T V Y L E R H I C G R L F G E K F R H F N A L G G W G E L Q N S V K T F G E T H P F T K L V V D L T D I D P D V A Y S S V P Y E K G F A L L F Y L E Q L L G G P E I F L G F L K A Y V E K F S Y K S I T T D D W K D F L Y S Y F K D K V D V L N Q V D W N A W L Y S P G L P P I K P N Y D M T L T N A C I A L S Q R W I T A K E D D L N S F N A T D L K D L S S H Q L N E F L A Q T L Q R A P L P L G H I K R M Q E V Y N F N A I N N S E I R F R W L R L C I Q S K W E D A I P L A L K M A T E Q G R M K F T R P L F K D L A A F D K S H D Q A V R T Y Q E H K A S M H P V T A M L V G K D L K V D D Y K D D D D K H H H H H H
- Accession
- P09960
- Protein length
- Full Length
- Predicted molecular weight
- 71 kDa
- Amino acids
- 1 to 611
- Nature
- Recombinant
- Tags
- DDDDK tag C-Terminus, His tag C-Terminus
Specifications
- Form
- Liquid
- Additional notes
ab198440 was purified by affinity chromatography.
General info
- Function
Bifunctional zinc metalloenzyme that comprises both epoxide hydrolase (EH) and aminopeptidase activities. Acts as an epoxide hydrolase to catalyze the conversion of LTA4 to the pro-inflammatory mediator leukotriene B4 (LTB4) (PubMed:11917124, PubMed:12207002, PubMed:15078870, PubMed:18804029, PubMed:1897988, PubMed:1975494, PubMed:2244921). Has also aminopeptidase activity, with high affinity for N-terminal arginines of various synthetic tripeptides (PubMed:18804029, PubMed:20813919). In addition to its pro-inflammatory EH activity, may also counteract inflammation by its aminopeptidase activity, which inactivates by cleavage another neutrophil attractant, the tripeptide Pro-Gly-Pro (PGP), a bioactive fragment of collagen generated by the action of matrix metalloproteinase-9 (MMP9) and prolylendopeptidase (PREPL) (PubMed:20813919, PubMed:24591641). Involved also in the biosynthesis of resolvin E1 and 18S-resolvin E1 from eicosapentaenoic acid, two lipid mediators that show potent anti-inflammatory and pro-resolving actions (PubMed:21206090).
- Sequence similarities
Belongs to the peptidase M1 family.
- Post-translational modifications
Phosphorylation at Ser-416 inhibits leukotriene-A4 hydrolase activity.
Storage
- Shipped at conditions
- Dry Ice
- Appropriate short-term storage conditions
- -80°C
- Appropriate long-term storage conditions
- -80°C
- Storage information
- Avoid freeze / thaw cycle
Notes
This product was previously labelled as Leukotriene A4 hydrolase
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Leukotriene A4 hydrolase often called LTA4H is an enzyme that converts leukotriene A4 into leukotriene B4 an important mediator in inflammation. It has a molecular mass of approximately 69 kDa and functions as a zinc metalloprotease. Expression of LTA4H is widespread detected in various tissues including the lung liver and kidney. This enzyme plays an essential role in modulating inflammatory responses due to its ability to catalyze the transformation of leukotriene A4.
- Biological function summary
Leukotriene A4 hydrolase (LTA4H) transforms leukotriene A4 an unstable epoxide into leukotriene B4 a potent chemoattractant. The enzyme does not function within a multi-protein complex but interacts with small molecules during leukotriene biosynthesis. Leukotriene B4 produced by LTA4H recruits neutrophils to sites of inflammation enhancing immune defense. While its primary function is promoting inflammation this activity also regulates the resolution phase by facilitating tissue repair and return to homeostasis.
- Pathways
Leukotriene A4 hydrolase plays a role in the arachidonic acid cascade an important biological pathway for synthesizing eicosanoids. This pathway involves several steps where arachidonic acid releases from cell membrane phospholipids and converts to intermediate leukotriene A4 by 5-lipoxygenase. LTA4H then converts leukotriene A4 into leukotriene B4 which orchestrates inflammatory responses. LTA4H connects to proteins like 5-lipoxygenase which also acts within this cascade.
- Associated diseases and disorders
Leukotriene A4 hydrolase associates with inflammatory diseases such as asthma and inflammatory bowel disease. In asthma the overproduction of leukotriene B4 can exacerbate airway inflammation and hyperresponsiveness. Here leukotrienes interact with receptors on effector cells which contributes to the pathology of asthma. Inflammatory bowel disease showcases a similar link where increased levels of leukotriene B4 potentiate intestinal inflammation. Effective management of these conditions may involve targeting leukotriene pathways specifically inhibiting the activity of LTA4H to ameliorate symptoms.
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SDS-PAGE - Recombinant Human Leukotriene A4 hydrolase/LTA4H protein (ab198440)
4-20% SDS-PAGE analysis of 2.8 μg ab198440 with Coomassie staining.
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