Recombinant Human LH1 protein is a Human Full Length protein, in the 1 to 727 aa range, expressed in Wheat germ and suitable for ELISA, WB.
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Key facts
- Expression system
- Wheat germ
- Tags
- GST tag N-Terminus
- Applications
- ELISA, WB
- Biologically active
- No
Amino acid sequence
M R P L L L L A L L G W L L L A E A K G D A K P E D N L L V L T V A T K E T E G F R R F K R S A Q F F N Y K I Q A L G L G E D W N V E K G T S A G G G Q K V R L L K K A L E K H A D K E D L V I L F A D S Y D V L F A S G P R E L L K K F R Q A R S Q V V F S A E E L I Y P D R R L E T K Y P V V S D G K R F L G S G G F I G Y A P N L S K L V A E W E G Q D S D S D Q L F Y T K I F L D P E K R E Q I N I T L D H R C R I F Q N L D G A L D E V V L K F E M G H V R A R N L A Y D T L P V L I H G N G P T K L Q L N Y L G N Y I P R F W T F E T G C T V C D E G L R S L K G I G D E A L P T V L V G V F I E Q P T P F V S L F F Q R L L R L H Y P Q K H M R L F I H N H E Q H H K A Q V E E F L A Q H G S E Y Q S V K L V G P E V R M A N A D A R N M G A D L C R Q D R S C T Y Y F S V D A D V A L T E P N S L R L L I Q Q N K N V I A P L M T R H G R L W S N F W G A L S A D G Y Y A R S E D Y V D I V Q G R R V G V W N V P Y I S N I Y L I K G S A L R G E L Q S S D L F H H S K L D P D M A F C A N I R Q Q D V F M F L T N R H T L G H L L S L D S Y R T T H L H N D L W E V F S N P E D W K E K Y I H Q N Y T K A L A G K L V E T P C P D V Y W F P I F T E V A C D E L V E E M E H F G Q W S L G N N K D N R I Q G G Y E N V P T I D I H M N Q I G F E R E W H K F L L E Y I A P M T E K L Y P G Y Y T R A Q F D L A F V V R Y K P D E Q P S L M P H H D A S T F T I N I A L N R V G V D Y E G G G C R F L R Y N C S I R A P R K G W T L M H P G R L T H Y H E G L P T T R G T R Y I A V S F V D P
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application ELISA | Reactivity Reacts | Dilution info - | Notes - |
Application WB | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Part of a complex composed of PLOD1, P3H3 and P3H4 that catalyzes hydroxylation of lysine residues in collagen alpha chains and is required for normal assembly and cross-linkling of collagen fibrils (By similarity). Forms hydroxylysine residues in -Xaa-Lys-Gly- sequences in collagens (PubMed:10686424, PubMed:15854030, PubMed:8621606). These hydroxylysines serve as sites of attachment for carbohydrate units and are essential for the stability of the intermolecular collagen cross-links (Probable).
Alternative names
LLH, PLOD, PLOD1, Lysyl hydroxylase 1, LH1
Recommended products
Recombinant Human LH1 protein is a Human Full Length protein, in the 1 to 727 aa range, expressed in Wheat germ and suitable for ELISA, WB.
Key facts
- Expression system
- Wheat germ
- Tags
- GST tag N-Terminus
- Applications
- ELISA, WB
- Biologically active
- No
- Accession
- Q02809-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 8
Constituents: 0.79% Tris HCl, 0.31% Glutathione
Sequence info
Amino acid sequence
- M R P L L L L A L L G W L L L A E A K G D A K P E D N L L V L T V A T K E T E G F R R F K R S A Q F F N Y K I Q A L G L G E D W N V E K G T S A G G G Q K V R L L K K A L E K H A D K E D L V I L F A D S Y D V L F A S G P R E L L K K F R Q A R S Q V V F S A E E L I Y P D R R L E T K Y P V V S D G K R F L G S G G F I G Y A P N L S K L V A E W E G Q D S D S D Q L F Y T K I F L D P E K R E Q I N I T L D H R C R I F Q N L D G A L D E V V L K F E M G H V R A R N L A Y D T L P V L I H G N G P T K L Q L N Y L G N Y I P R F W T F E T G C T V C D E G L R S L K G I G D E A L P T V L V G V F I E Q P T P F V S L F F Q R L L R L H Y P Q K H M R L F I H N H E Q H H K A Q V E E F L A Q H G S E Y Q S V K L V G P E V R M A N A D A R N M G A D L C R Q D R S C T Y Y F S V D A D V A L T E P N S L R L L I Q Q N K N V I A P L M T R H G R L W S N F W G A L S A D G Y Y A R S E D Y V D I V Q G R R V G V W N V P Y I S N I Y L I K G S A L R G E L Q S S D L F H H S K L D P D M A F C A N I R Q Q D V F M F L T N R H T L G H L L S L D S Y R T T H L H N D L W E V F S N P E D W K E K Y I H Q N Y T K A L A G K L V E T P C P D V Y W F P I F T E V A C D E L V E E M E H F G Q W S L G N N K D N R I Q G G Y E N V P T I D I H M N Q I G F E R E W H K F L L E Y I A P M T E K L Y P G Y Y T R A Q F D L A F V V R Y K P D E Q P S L M P H H D A S T F T I N I A L N R V G V D Y E G G G C R F L R Y N C S I R A P R K G W T L M H P G R L T H Y H E G L P T T R G T R Y I A V S F V D P
- Accession
- Q02809
- Protein length
- Full Length
- Amino acids
- 1 to 727
- Nature
- Recombinant
- Tags
- GST tag N-Terminus
Specifications
- Form
- Liquid
General info
- Function
Part of a complex composed of PLOD1, P3H3 and P3H4 that catalyzes hydroxylation of lysine residues in collagen alpha chains and is required for normal assembly and cross-linkling of collagen fibrils (By similarity). Forms hydroxylysine residues in -Xaa-Lys-Gly- sequences in collagens (PubMed:10686424, PubMed:15854030, PubMed:8621606). These hydroxylysines serve as sites of attachment for carbohydrate units and are essential for the stability of the intermolecular collagen cross-links (Probable).
Storage
- Shipped at conditions
- Dry Ice
- Appropriate short-term storage conditions
- -80°C
- Appropriate long-term storage conditions
- -80°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
This product was previously labelled as PLOD1.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
LH1 also known as Luteinizing Hormone subunit beta plays an important mechanical role in reproductive biology. The molecular mass of LH1 is approximately 15 kDa. It is chiefly expressed in the anterior pituitary gland. This expression is essential for mediating reproductive processes by interacting with other hormones and receptors located in various tissues including the gonads. The activity of LH1 involves binding and signaling through specific hormone receptors therefore instigating a cascade of biological responses.
- Biological function summary
LH1 engages in regulatory functions within the reproductive system facilitating ovulation and stimulating the production of sex steroids such as testosterone and estradiol. LH1 operates as part of a dimeric glycoprotein hormone complex which includes the alpha and beta subunits that also construct other glycoprotein hormones like TSH and FSH. This complex plays a fundamental role in orchestrating the hormonal milieu necessary for reproductive organ function and development.
- Pathways
LH1 operates within the hypothalamic-pituitary-gonadal (HPG) axis. This pathway involves a series of hormonal signals starting from GnRH release from the hypothalamus prompting the synthesis and release of LH1 from the pituitary. Within this axis LH1 works in conjunction with related proteins like FSH to regulate reproductive functions. These pathways ensure proper feedback regulation of hormone levels important for maintaining reproductive health and function.
- Associated diseases and disorders
LH1 abnormalities have associations with reproductive conditions such as polycystic ovary syndrome (PCOS) and hypogonadism. In PCOS an imbalance in LH1 and FSH levels can disrupt normal ovarian function leading to symptoms like irregular menstruation and infertility. In hypogonadism LH1 levels may be insufficient to stimulate normal gonadal activity. Prolactin another hormone interacting in these disorders can also influence the functioning of LH1 adding complexity to the underlying pathophysiology.
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1 product image
SDS-PAGE - Recombinant Human LH1 protein (ab159142)
ab159142 on a 12.5% SDS-PAGE stained with Coomassie Blue.
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