Recombinant Human LIF protein (Active)
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Recombinant Human LIF protein (Active) is a Human Full Length protein, in the 23 to 202 aa range, expressed in HEK 293 cells, with >95%, <0.005 EU/µg endotoxin level, suitable for SDS-PAGE, FuncS, Mass Spec, HPLC.
View Alternative Names
HILDA, LIF, Leukemia inhibitory factor, Differentiation-stimulating factor, Melanoma-derived LPL inhibitor, D factor, MLPLI
- Mass Spec
Supplier Data
Mass Spectrometry - Recombinant Human LIF protein (Active) (AB287941)
ESI-TOF analysis of ab287941.
Predicted MW is 19772.92 Da (+/- 10 Da by ESI-TOF). Observed MW is 19777.88 Da.
- FuncS
Supplier Data
Functional Studies - Recombinant Human LIF protein (Active) (AB287941)
Functional analysis of ab287941.
Fully biologically active determined by the dose dependent proliferation of TF-1 cells. ED50 is ≤0.16 ng/mL, corresponding to a specific activity of of 6.25 x 106 units/mg.
Cell based assay testing is performed on the first lot of protein only and is provided as a reference for protein activity; subsequent lots of protein must pass all biophysical quality control parameters that meet the same parameters as the first lot.
Lot GR3424610-1
- FuncS
Lab
Functional Studies - Recombinant Human LIF protein (Active) (AB287941)
Fully biologically active determined by dose dependent proliferation of TF-1 cells. ED50 is ≤ 0.16 ng/ml, corresponding to a specific activity of 6.25x106 units/mg. Cell based assay testing is performed on the first lot of protein only and is provided as a reference for protein activity; subsequent lots of protein must pass all biophysical quality control parameters that meet the same parameters as the first lot. Lot GR3424610-1
- HPLC
Supplier Data
HPLC - Recombinant Human LIF protein (Active) (AB287941)
HPLC analysis of ab287941.
- SDS-PAGE
Supplier Data
SDS-PAGE - Recombinant Human LIF protein (Active) (AB287941)
SDS-PAGE analysis of ab287941.
Reactivity data
Sequence info
Properties and storage information
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
LIF influences cell differentiation growth and inflammatory response. As part of the interleukin-6 cytokine family LIF exerts its effects through a receptor complex involving LIFR (LIF receptor) and GP130. The LIF protein's signaling controls processes like neuronal cell differentiation and bone formation. It impacts hematopoietic cells and supports embryonic implantation and placental development by preparing the uterine environment.
Pathways
The LIF protein is essential in the JAK/STAT signaling pathway which regulates gene expression vital for survival and proliferation. It also interacts with the MAPK/ERK pathway ensuring diverse cellular responses. These pathways link LIF to related proteins like STAT3 and ERK1/2 which mediate further downstream effects. The interplay between these signaling cascades highlights LIF's role in managing cell survival and proliferation in various contexts.
Specifications
Form
Lyophilized
Additional notes
Purity by HPLC =95%
General info
Function
LIF has the capacity to induce terminal differentiation in leukemic cells. Its activities include the induction of hematopoietic differentiation in normal and myeloid leukemia cells, the induction of neuronal cell differentiation, and the stimulation of acute-phase protein synthesis in hepatocytes.
Sequence similarities
Belongs to the LIF/OSM family.
Target data
Complementary Products
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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