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AB167895

Recombinant Human LIMD2 protein (His tag N-Terminus)

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(1 Publication)

Recombinant Human LIMD2 protein (His tag N-Terminus) is a Human Full Length protein, in the 1 to 127 aa range, expressed in Escherichia coli, with >90%, suitable for SDS-PAGE, Mass Spec.

View Alternative Names

SB143, LIMD2, LIM domain-containing protein 2

1 Images
SDS-PAGE - Recombinant Human LIMD2 protein (His tag N-Terminus) (AB167895)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant Human LIMD2 protein (His tag N-Terminus) (AB167895)

SDS-PAGE analysis of ab167895 (3 μg) under reducing conditions and visualized by coomassie blue stain.

Key facts

Purity

>90% SDS-PAGE

ab167895 is purified using conventional chromatography techniques.

Expression system

Escherichia coli

Tags

His tag N-Terminus

Applications

Mass Spec, SDS-PAGE

applications

Biologically active

No

Accession

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 7.4 Constituents: PBS, 20% Glycerol (glycerin, glycerine)

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "Mass Spec": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Sequence info

[{"linker":null,"sequence":"MGSSHHHHHHSSGLVPRGSHMGSMFQAAGAAQATPSHDAKGGGSSTVQRSKSFSLRAQVKETCAACQKTVYPMERLVADKLIFHNSCFCCKHCHTKLSLGSYAALHGEFYCKPHFQQLFKSKGNYDEGFGRKQHKELWAHKEVDPGTKTA","proteinLength":"Full Length","predictedMolecularWeight":"16.5 kDa","actualMolecularWeight":null,"aminoAcidEnd":127,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"Q9BT23","tags":[{"tag":"His","terminus":"N-Terminus"}]}]
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Properties and storage information

Form
Liquid
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

LIMD2 also known as LIM domain-containing protein 2 acts as a molecular adapter involved in cellular processes. It has a mass of approximately 14 kDa and exhibits widespread expression in various tissues including tumor tissues. This protein contains a single LIM domain which functions in protein-protein interactions allowing it to interact with multiple binding partners.
Biological function summary

The protein plays a significant role in cellular adhesion and migration. LIMD2 directly influences the structure and dynamics of the cytoskeleton which are important for cellular movement. It can form part of multiprotein complexes where it interacts with cytoskeletal proteins and signaling molecules. LIMD2 modulates actin cytoskeleton and focal adhesion which are critical for cell motility and invasiveness.

Pathways

The protein is significantly involved in metastasis and integrin signaling pathways. LIMD2 interacts with integrin-linked kinase (ILK) impacting cell adhesion and spreading processes. It is also associated with the MAPK pathway where it may link to ERK influencing cell proliferation and differentiation. Through these interactions LIMD2 participates in complex signaling networks that regulate critical cellular functions.

LIMD2 has a strong connection with cancer metastasis and progression. Its expression correlates with tumor aggressiveness and poor prognosis in certain cancers including melanoma and bladder cancer. The protein connects with other cancer-related proteins such as integrin and ILK contributing to enhanced migratory and invasive characteristics of cancer cells. Targeting LIMD2 offers potential therapeutic strategies for limiting cancer metastasis.
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General info

Function

Acts as an activator of the protein-kinase ILK, thereby regulating cell motility (PubMed : 24590809).

Subcellular localisation

Nucleus

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Product protocols

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Target data

Acts as an activator of the protein-kinase ILK, thereby regulating cell motility (PubMed : 24590809).
See full target information LIMD2
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Bioengineered 12:10089-10100 PubMed34724866

2021

LIM domain-containing 2 (LIMD2) promotes the progress of ovarian cancer via the focal adhesion signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Lixin Chen,Ji Qian,Qinghua You,Jie Ma
View all publications
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Product promise

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