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AB207944

Recombinant human LIMK2 protein

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Recombinant human LIMK2 protein is a Human Full Length protein, in the 1 to 638 aa range, expressed in Baculovirus infected Sf9 cells, with >85%, suitable for SDS-PAGE, FuncS.

View Alternative Names

LIM domain kinase 2, LIMK-2, LIMK2

2 Images
Functional Studies - Recombinant human LIMK2 protein (AB207944)
  • FuncS

Supplier Data

Functional Studies - Recombinant human LIMK2 protein (AB207944)

Sample Kinase Activity assay plot using ab207944, showing the specific activity to be 3 nmol/min/mg.

SDS-PAGE - Recombinant human LIMK2 protein (AB207944)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant human LIMK2 protein (AB207944)

SDS-PAGE analysis of ab207944.

Key facts

Purity

>85% Densitometry

Expression system

Baculovirus infected Sf9 cells

Tags

GST tag N-Terminus

Applications

SDS-PAGE, FuncS

applications

Biologically active

Yes

Biological activity

The specific activity of ab207944 was found to be 3 nmol/min/mg.

Accession

P53671

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 7.5 Constituents: 25% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, 0.79% Tris HCl, 0.31% Glutathione, 0.004% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.003% EDTA, 0.002% PMSF

storage-buffer

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Recombinant human LIM Kinase 1 protein

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Primary Antibodies

AB45165

Anti-LIMK2 antibody [EP969Y] - C-terminal

RabMAb|Recombinant|KO Validated|Trial Size

Immunocytochemistry/ Immunofluorescence - Anti-LIMK2 antibody [EP969Y] - C-terminal (AB45165)

  • 3
  • 2
Primary Antibodies

AB75748

Anti-EEF2/Elongation factor 2 antibody [EP880Y]

RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EEF2/Elongation factor 2 antibody [EP880Y] (AB75748)

  • 0
  • 0
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "FuncS": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Previously labelled as LIM kinase 2.
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Sequence info

[{"sequence":"MSALAGEDVWRCPGCGDHIAPSQIWYRTVNETWHGSCFRCSECQDSLTNWYYEKDGKLYCPKDYWGKFGEFCHGCSLLMTGPFMVAGEFKYHPECFACMSCKVIIEDGDAYALVQHATLYCGKCHNEVVLAPMFERLSTESVQEQLPYSVTLISMPATTEGRRGFSVSVESACSNYATTVQVKEVNRMHISPNNRNAIHPGDRILEINGTPVRTLRVEEVEDAISQTSQTLQLLIEHDPVSQRLDQLRLEARLAPHMQNAGHPHALSTLDTKENLEGTLRRRSLRRSNSISKSPGPSSPKEPLLFSRDISRSESLRCSSSYSQQIFRPCDLIHGEVLGKGFFGQAIKVTHKATGKVMVMKELIRCDEETQKTFLTEVKVMRSLDHPNVLKFIGVLYKDKKLNLLTEYIEGGTLKDFLRSMDPFPWQQKVRFAKGIASGMAYLHSMCIIHRDLNSHNCLIKLDKTVVVADFGLSRLIVEERKRAPMEKATTKKRTLRKNDRKKRYTVVGNPYWMAPEMLNGKSYDETVDIFSFGIVLCEIIGQVYADPDCLPRTLDFGLNVKLFWEKFVPTDCPPAFFPLAAICCRLEPESRPAFSKLEDSFEALSLYLGELGIPLPAELEELDHTVSMQYGLTRDSPP","proteinLength":"Full Length","predictedMolecularWeight":"96 kDa","actualMolecularWeight":null,"aminoAcidEnd":638,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Baculovirus infected Sf9 cells","accessionNumber":"P53671","tags":[{"tag":"GST","terminus":"N-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Dry Ice
Appropriate short-term storage conditions
-80°C
Appropriate long-term storage conditions
-80°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
True
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

LIMK2 also known as LIM domain kinase 2 is a protein that functions primarily as a serine/threonine kinase. This protein with a molecular weight of approximately 72 kDa plays an important role in regulating the cytoskeleton dynamics by phosphorylating and inactivating cofilin thereby affecting actin filament stability. LIMK2 is ubiquitously expressed with higher expression levels observed in the brain and certain types of cancer tissues indicating its importance in both normal and pathological cell processes.
Biological function summary

LIMK2 influences cell motility differentiation and division by modulating the actin cytoskeleton. It is a central component in various signaling complexes where it interacts with proteins such as ROCK and PAK which are upstream activators. By controlling actin filament assembly and disassembly LIMK2 impacts essential cellular functions including migration and invasion highlighting its potential role in cancer metastasis.

Pathways

LIMK2 integrates into signaling cascades linked to the Rho family of GTPases and the TGF-beta pathways. In these pathways it collaborates with proteins like ROCK to influence cytoskeletal reorganization. LIMK2's activity in these pathways emphasizes its role in maintaining cellular integrity and responding to extracellular stimuli which are critical components of cell cycle regulation and morphological changes.

Aberrant LIMK2 activity has connections with cancer progression and neurological disorders. In cancer upregulation can lead to increased tumor cell invasion and metastasis through its interaction with cofilin and the subsequent increase in actin filament assembly. In neurological contexts altered LIMK2 function associates with neurodegenerative diseases possibly due to its role in neuronal signaling networks and cytoskeletal dynamics. Understanding LIMK2’s interactions with proteins like cofilin offers insight into potential therapeutic targets for managing these diseases.
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Specifications

Form

Liquid

Additional notes

Affinity purified.

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General info

Function

Serine/threonine-protein kinase that plays an essential role in the regulation of actin filament dynamics (PubMed : 10436159, PubMed : 11018042). Acts downstream of several Rho family GTPase signal transduction pathways (PubMed : 10436159, PubMed : 11018042). Involved in astral microtubule organization and mitotic spindle orientation during early stages of mitosis by mediating phosphorylation of TPPP (PubMed : 22328514). Displays serine/threonine-specific phosphorylation of myelin basic protein and histone (MBP) in vitro (PubMed : 8537403). Suppresses ciliogenesis via multiple pathways; phosphorylation of CFL1, suppression of directional trafficking of ciliary vesicles to the ciliary base, and by facilitating YAP1 nuclear localization where it acts as a transcriptional corepressor of the TEAD4 target genes AURKA and PLK1 (PubMed : 25849865).

Sequence similarities

Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family.

Post-translational modifications

Phosphorylated on serine and/or threonine residues by ROCK1.

Subcellular localisation

Cytoskeleton

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Product protocols

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Target data

Serine/threonine-protein kinase that plays an essential role in the regulation of actin filament dynamics (PubMed : 10436159, PubMed : 11018042). Acts downstream of several Rho family GTPase signal transduction pathways (PubMed : 10436159, PubMed : 11018042). Involved in astral microtubule organization and mitotic spindle orientation during early stages of mitosis by mediating phosphorylation of TPPP (PubMed : 22328514). Displays serine/threonine-specific phosphorylation of myelin basic protein and histone (MBP) in vitro (PubMed : 8537403). Suppresses ciliogenesis via multiple pathways; phosphorylation of CFL1, suppression of directional trafficking of ciliary vesicles to the ciliary base, and by facilitating YAP1 nuclear localization where it acts as a transcriptional corepressor of the TEAD4 target genes AURKA and PLK1 (PubMed : 25849865).
See full target information LIMK2
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