Recombinant Human Lin28A protein (Tagged) is a Human Full Length protein, in the 2 to 209 aa range, expressed in HEK 293, with >=70% purity and suitable for SDS-PAGE.
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Publications
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- International journal of molecular sciences 23:2022Ontogenetic Changes in the Expression of the Lin28 Protein in the Rat Hypothalamic Tuberal Nuclei.Applications:Unspecified applicationReactive species:Unspecified reactive speciesPolina A Anfimova,Lydia G Pankrasheva,Konstantin Yu Moiseev,Elizaveta S Shirina,Valentina V Porseva,Petr M MasliukovPubMed 36362250
Key facts
- Purity
- >=70% SDS-PAGE
- Expression system
- HEK 293 cells
- Tags
- Avi tag N-Terminus DDDDK tag N-Terminus
- Applications
- SDS-PAGE
- Biologically active
- No
Amino acid sequence
G S V S N Q Q F A G G C A K A A E E A P E E A P E D A A R A A D E P Q L L H G A G I C K W F N V R M G F G F L S M T A R A G V A L D P P V D V F V H Q S K L H M E G F R S L K E G E A V E F T F K K S A K G L E S I R V T G P G G V F C I G S E R R P K G K S M Q K R R S K G D R C Y N C G G L D H H A K E C K L P P Q P K K C H F C Q S I S H M V A S C P L K A Q Q G P S A Q G K P T Y F R E E E E E I H S P T L L P E A Q N
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
RNA-binding protein that inhibits processing of pre-let-7 miRNAs and regulates translation of mRNAs that control developmental timing, pluripotency and metabolism (PubMed:21247876). Seems to recognize a common structural G-quartet (G4) feature in its miRNA and mRNA targets (Probable). 'Translational enhancer' that drives specific mRNAs to polysomes and increases the efficiency of protein synthesis. Its association with the translational machinery and target mRNAs results in an increased number of initiation events per molecule of mRNA and, indirectly, in mRNA stabilization. Binds IGF2 mRNA, MYOD1 mRNA, ARBP/36B4 ribosomal protein mRNA and its own mRNA. Essential for skeletal muscle differentiation program through the translational up-regulation of IGF2 expression. Suppressor of microRNA (miRNA) biogenesis, including that of let-7, miR107, miR-143 and miR-200c. Specifically binds the miRNA precursors (pre-miRNAs), recognizing an 5'-GGAG-3' motif found in pre-miRNA terminal loop, and recruits TUT4 and TUT7 uridylyltransferases (PubMed:18951094, PubMed:19703396, PubMed:22118463, PubMed:22898984). This results in the terminal uridylation of target pre-miRNAs (PubMed:18951094, PubMed:19703396, PubMed:22118463, PubMed:22898984). Uridylated pre-miRNAs fail to be processed by Dicer and undergo degradation. The repression of let-7 expression is required for normal development and contributes to maintain the pluripotent state by preventing let-7-mediated differentiation of embryonic stem cells (PubMed:18951094, PubMed:19703396, PubMed:22118463, PubMed:22898984). Localized to the periendoplasmic reticulum area, binds to a large number of spliced mRNAs and inhibits the translation of mRNAs destined for the ER, reducing the synthesis of transmembrane proteins, ER or Golgi lumen proteins, and secretory proteins. Binds to and enhances the translation of mRNAs for several metabolic enzymes, such as PFKP, PDHA1 or SDHA, increasing glycolysis and oxidative phosphorylation. Which, with the let-7 repression may enhance tissue repair in adult tissue (By similarity).
Alternative names
CSDD1, LIN28, ZCCHC1, LIN28A, Protein lin-28 homolog A, Lin-28A, Zinc finger CCHC domain-containing protein 1
Recommended products
Recombinant Human Lin28A protein (Tagged) is a Human Full Length protein, in the 2 to 209 aa range, expressed in HEK 293, with >=70% purity and suitable for SDS-PAGE.
Key facts
- Purity
- >=70% SDS-PAGE
- Expression system
- HEK 293 cells
- Tags
- Avi tag N-Terminus DDDDK tag N-Terminus
- Applications
- SDS-PAGE
- Biologically active
- No
- Accession
- Q9H9Z2-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 8
Constituents: 20% Glycerol (glycerin, glycerine), 0.64% Sodium chloride, 0.63% Tris HCl, 0.02% Potassium chloride
Sequence info
Amino acid sequence
- G S V S N Q Q F A G G C A K A A E E A P E E A P E D A A R A A D E P Q L L H G A G I C K W F N V R M G F G F L S M T A R A G V A L D P P V D V F V H Q S K L H M E G F R S L K E G E A V E F T F K K S A K G L E S I R V T G P G G V F C I G S E R R P K G K S M Q K R R S K G D R C Y N C G G L D H H A K E C K L P P Q P K K C H F C Q S I S H M V A S C P L K A Q Q G P S A Q G K P T Y F R E E E E E I H S P T L L P E A Q N
- Accession
- Q9H9Z2
- Protein length
- Full Length
- Predicted molecular weight
- 26 kDa
- Amino acids
- 2 to 209
- Nature
- Recombinant
- Tags
- Avi tag N-Terminus, DDDDK tag N-Terminus
Specifications
- Form
- Liquid
General info
- Function
RNA-binding protein that inhibits processing of pre-let-7 miRNAs and regulates translation of mRNAs that control developmental timing, pluripotency and metabolism (PubMed:21247876). Seems to recognize a common structural G-quartet (G4) feature in its miRNA and mRNA targets (Probable). 'Translational enhancer' that drives specific mRNAs to polysomes and increases the efficiency of protein synthesis. Its association with the translational machinery and target mRNAs results in an increased number of initiation events per molecule of mRNA and, indirectly, in mRNA stabilization. Binds IGF2 mRNA, MYOD1 mRNA, ARBP/36B4 ribosomal protein mRNA and its own mRNA. Essential for skeletal muscle differentiation program through the translational up-regulation of IGF2 expression. Suppressor of microRNA (miRNA) biogenesis, including that of let-7, miR107, miR-143 and miR-200c. Specifically binds the miRNA precursors (pre-miRNAs), recognizing an 5'-GGAG-3' motif found in pre-miRNA terminal loop, and recruits TUT4 and TUT7 uridylyltransferases (PubMed:18951094, PubMed:19703396, PubMed:22118463, PubMed:22898984). This results in the terminal uridylation of target pre-miRNAs (PubMed:18951094, PubMed:19703396, PubMed:22118463, PubMed:22898984). Uridylated pre-miRNAs fail to be processed by Dicer and undergo degradation. The repression of let-7 expression is required for normal development and contributes to maintain the pluripotent state by preventing let-7-mediated differentiation of embryonic stem cells (PubMed:18951094, PubMed:19703396, PubMed:22118463, PubMed:22898984). Localized to the periendoplasmic reticulum area, binds to a large number of spliced mRNAs and inhibits the translation of mRNAs destined for the ER, reducing the synthesis of transmembrane proteins, ER or Golgi lumen proteins, and secretory proteins. Binds to and enhances the translation of mRNAs for several metabolic enzymes, such as PFKP, PDHA1 or SDHA, increasing glycolysis and oxidative phosphorylation. Which, with the let-7 repression may enhance tissue repair in adult tissue (By similarity).
- Sequence similarities
Belongs to the lin-28 family.
- Subcellular localisation
- Nucleus, Nucleolus
Storage
- Shipped at conditions
- Dry Ice
- Appropriate short-term storage conditions
- -80°C
- Appropriate long-term storage conditions
- -80°C
- Storage information
- Avoid freeze / thaw cycle
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1 product image
SDS-PAGE - Recombinant Human Lin28A protein (Tagged) (ab271598)
SDS-PAGE analysis of 2 μg ab271598.
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