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AB188506

Recombinant Human LIPG protein

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Recombinant Human LIPG protein is a Human Full Length protein, in the 21 to 500 aa range, expressed in HEK 293 cells, with >85%, < 1 EU/µg endotoxin level, suitable for SDS-PAGE, ELISA, Mass Spec.

View Alternative Names

UNQ387/PRO719, LIPG, Endothelial lipase, Endothelial cell-derived lipase, Phospholipase A1, EDL, EL

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SDS-PAGE - Recombinant Human LIPG protein (AB188506)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant Human LIPG protein (AB188506)

SDS PAGE analysis of ab188506

Lane 1 : Reduced and boiled sample, 2.5 μg/lane
Lane 2 : Non-reduced and non-boiled sample, 2.5 μg/lane

Key facts

Purity

>85% Densitometry

Endotoxin level

< 1 EU/µg

Expression system

HEK 293 cells

Tags

His tag C-Terminus

Applications

SDS-PAGE, ELISA, Mass Spec

applications

Biologically active

No

Accession

Q9Y5X9

Animal free

No

Carrier free

No

Species

Human

Reconstitution

Reconstitute in 200 µL of water

Storage buffer

Constituents: PBS, 4% Mannitol, 1% Sucrose

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "ELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "Mass Spec": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Sequence info

[{"sequence":"ASSPVPFGPEGRLEDKLHKPKATQTEVKPSVRFNLRTSKDPEHEGCYLSVGHSQPLEDCSFNMTAKTFFIIHGWTMSGIFENWLHKLVSALHTREKDANVVVVDWLPLAHQLYTDAVNNTRVVGHSIARMLDWLQEKDDFSLGNVHLIGYSLGAHVAGYAGNFVKGTVGRITGLDPAGPMFEGADIHKRLSPDDADFVDVLHTYTRSFGLSIGIQMPVGHIDIYPNGGDFQPGCGLNDVLGSIAYGTITEVVKCEHERAVHLFVDSLVNQDKPSFAFQCTDSNRFKKGICLSCRKNRCNSIGYNAKKMRNKRNSKMYLKTRAGMPFRVYHYQMKIHVFSYKNMGEIEPTFYVTLYGTNADSQTLPLEIVERIEQNATNTFLVYTEEDLGDLLKIQLTWEGASQSWYNLWKEFRSYLSQPRNPGRELNIRRIRVKSGETQRKLTFCTEDPENTSISPGRELWFRKCRDGWRMKNETSPTVELPKLHHHHHH","proteinLength":"Full Length","predictedMolecularWeight":"55.8 kDa","actualMolecularWeight":null,"aminoAcidEnd":500,"aminoAcidStart":21,"nature":"Recombinant","expressionSystem":"HEK 293 cells","accessionNumber":"Q9Y5X9","tags":[{"tag":"His","terminus":"C-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-80°C
Appropriate long-term storage conditions
-80°C
Storage information
Avoid freeze / thaw cycle
False
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

LIPG also known as endothelial lipase or the LIPG protein is an important enzyme involved in lipid metabolism. It belongs to the triglyceride lipase gene family and is mainly responsible for hydrolyzing phospholipids. The protein has a mass of about 55 kDa and is expressed primarily in endothelial cells that line blood vessels but also found in other tissues like the liver and lungs. This enzyme plays a role in cholesterol metabolism by impacting high-density lipoprotein (HDL) cholesterol levels influencing cardiovascular health.
Biological function summary

LIPG protein serves a role in lipid and lipoprotein metabolism. It does not operate as part of a larger complex but influences the remodeling of high-density lipoproteins by hydrolyzing their phospholipid content. By doing so LIPG helps regulate the levels and distribution of lipoproteins in circulation. This can affect the levels of free fatty acids and consequently influence energy homeostasis.

Pathways

LIPG protein participates in the lipoprotein metabolism and cholesterol transport pathways. It acts alongside other lipases such as hepatic lipase and lipoprotein lipase to maintain lipid homeostasis. These pathways are critical for the mobilization and distribution of fatty acids and cholesterol in the body. LIPG's modulation of HDL cholesterol levels links it to pathways of both cardiovascular and metabolic health through interactions with cholesterol transport proteins.

LIPG protein is closely associated with atherosclerosis and cardiovascular diseases. Altered activity or expression of LIPG can contribute to the development of these conditions by affecting HDL cholesterol levels. Disease pathways involve its interaction with other proteins such as ApoA-I a major component of HDL which impacts lipid transport and cardiovascular risk. Understanding LIPG's role in these disorders can potentially lead to new therapeutic approaches targeting lipid metabolism.
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Specifications

Form

Lyophilized

Additional notes

ab188506 was purifed using Ni-NTA chromatography.

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General info

Function

Exerts both phospholipase and triglyceride lipase activities (PubMed : 10192396, PubMed : 10318835, PubMed : 12032167). More active as a phospholipase than a triglyceride lipase (PubMed : 12032167). Hydrolyzes triglycerides, both with short-chain fatty acyl groups (tributyrin) and long-chain fatty acyl groups (triolein) with similar levels of activity toward both types of substrates (PubMed : 12032167). Hydrolyzes high density lipoproteins (HDL) more efficiently than other lipoproteins (PubMed : 10192396, PubMed : 12032167).

Sequence similarities

Belongs to the AB hydrolase superfamily. Lipase family.

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Product protocols

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Target data

Exerts both phospholipase and triglyceride lipase activities (PubMed : 10192396, PubMed : 10318835, PubMed : 12032167). More active as a phospholipase than a triglyceride lipase (PubMed : 12032167). Hydrolyzes triglycerides, both with short-chain fatty acyl groups (tributyrin) and long-chain fatty acyl groups (triolein) with similar levels of activity toward both types of substrates (PubMed : 12032167). Hydrolyzes high density lipoproteins (HDL) more efficiently than other lipoproteins (PubMed : 10192396, PubMed : 12032167).
See full target information LIPG
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