JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB180286

Recombinant Human LIPG protein (denatured) (His tag N-Terminus)

Be the first to review this product! Submit a review

|

(0 Publication)

Recombinant Human LIPG protein (denatured) (His tag N-Terminus) is a Human Fragment protein, in the 21 to 340 aa range, expressed in Escherichia coli, with >85%, suitable for SDS-PAGE.

View Alternative Names

UNQ387/PRO719, LIPG, Endothelial lipase, Endothelial cell-derived lipase, Phospholipase A1, EDL, EL

1 Images
SDS-PAGE - Recombinant Human LIPG protein (denatured) (His tag N-Terminus) (AB180286)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant Human LIPG protein (denatured) (His tag N-Terminus) (AB180286)

15% SDS-PAGE (3ug)

Key facts

Purity

>85% SDS-PAGE

Expression system

Escherichia coli

Tags

His tag N-Terminus

Applications

SDS-PAGE

applications

Biologically active

No

Accession

Q9Y5X9

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 8 Constituents: 10% Glycerol (glycerin, glycerine), 2.4% Urea, 0.32% Tris HCl

storage-buffer

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
style
left-column
style
left-column

Sequence info

[{"sequence":"MGSSHHHHHHSSGLVPRGSHMGSSPVPFGPEGRLEDKLHKPKATQTEVKPSVRFNLRTSKDPEHEGCYLSVGHSQPLEDCSFNMTAKTFFIIHGWTMSGIFENWLHKLVSALHTREKDANVVVVDWLPLAHQLYTDAVNNTRVVGHSIARMLDWLQEKDDFSLGNVHLIGYSLGAHVAGYAGNFVKGTVGRITGLDPAGPMFEGADIHKRLSPDDADFVDVLHTYTRSFGLSIGIQMPVGHIDIYPNGGDFQPGCGLNDVLGSIAYGTITEVVKCEHERAVHLFVDSLVNQDKPSFAFQCTDSNRFKKGICLSCRKNRCNSIGYNAKKMRNKRNSKMYLKTRA","proteinLength":"Fragment","predictedMolecularWeight":"38 kDa","actualMolecularWeight":null,"aminoAcidEnd":340,"aminoAcidStart":21,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"Q9Y5X9","tags":[{"tag":"His","terminus":"N-Terminus"}]}]
style
left-column

Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

LIPG also known as endothelial lipase or the LIPG protein is an important enzyme involved in lipid metabolism. It belongs to the triglyceride lipase gene family and is mainly responsible for hydrolyzing phospholipids. The protein has a mass of about 55 kDa and is expressed primarily in endothelial cells that line blood vessels but also found in other tissues like the liver and lungs. This enzyme plays a role in cholesterol metabolism by impacting high-density lipoprotein (HDL) cholesterol levels influencing cardiovascular health.
Biological function summary

LIPG protein serves a role in lipid and lipoprotein metabolism. It does not operate as part of a larger complex but influences the remodeling of high-density lipoproteins by hydrolyzing their phospholipid content. By doing so LIPG helps regulate the levels and distribution of lipoproteins in circulation. This can affect the levels of free fatty acids and consequently influence energy homeostasis.

Pathways

LIPG protein participates in the lipoprotein metabolism and cholesterol transport pathways. It acts alongside other lipases such as hepatic lipase and lipoprotein lipase to maintain lipid homeostasis. These pathways are critical for the mobilization and distribution of fatty acids and cholesterol in the body. LIPG's modulation of HDL cholesterol levels links it to pathways of both cardiovascular and metabolic health through interactions with cholesterol transport proteins.

LIPG protein is closely associated with atherosclerosis and cardiovascular diseases. Altered activity or expression of LIPG can contribute to the development of these conditions by affecting HDL cholesterol levels. Disease pathways involve its interaction with other proteins such as ApoA-I a major component of HDL which impacts lipid transport and cardiovascular risk. Understanding LIPG's role in these disorders can potentially lead to new therapeutic approaches targeting lipid metabolism.
style
left-column

Specifications

Form

Liquid

style
left-column

General info

Function

Exerts both phospholipase and triglyceride lipase activities (PubMed : 10192396, PubMed : 10318835, PubMed : 12032167). More active as a phospholipase than a triglyceride lipase (PubMed : 12032167). Hydrolyzes triglycerides, both with short-chain fatty acyl groups (tributyrin) and long-chain fatty acyl groups (triolein) with similar levels of activity toward both types of substrates (PubMed : 12032167). Hydrolyzes high density lipoproteins (HDL) more efficiently than other lipoproteins (PubMed : 10192396, PubMed : 12032167).

Sequence similarities

Belongs to the AB hydrolase superfamily. Lipase family.

style
left-column

Product protocols

style
left-column

Target data

Exerts both phospholipase and triglyceride lipase activities (PubMed : 10192396, PubMed : 10318835, PubMed : 12032167). More active as a phospholipase than a triglyceride lipase (PubMed : 12032167). Hydrolyzes triglycerides, both with short-chain fatty acyl groups (tributyrin) and long-chain fatty acyl groups (triolein) with similar levels of activity toward both types of substrates (PubMed : 12032167). Hydrolyzes high density lipoproteins (HDL) more efficiently than other lipoproteins (PubMed : 10192396, PubMed : 12032167).
See full target information LIPG
style
left-column
style
left-column
style
right-column

Complementary Products

Proteins & Peptides

AB78874

Recombinant human PPIH protein (Tag Free)

Bioactive

SDS-PAGE - Recombinant human PPIH protein (AB78874)

  • 0
  • 0
Proteins & Peptides

AB313361

Recombinant Human WFDC2 Protein (His tag)

Mass Spectrometry - Recombinant Human WFDC2 Protein (His tag) (AB313361)

  • 0
  • 0
Proteins & Peptides

AB217407

Recombinant human Vitronectin/S-Protein (Animal Free)

  • 0
  • 0
Proteins & Peptides

AB317877

Recombinant Human UCH-L1/PGP9.5 Protein (His-tag)

Mass Spectrometry - Recombinant Human UCH-L1/PGP9.5 Protein (His-tag) (AB317877)

  • 0
  • 0

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com