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AB93679

Recombinant Human Lipoamide Dehydrogenase protein

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Recombinant Human Lipoamide Dehydrogenase protein is a Human Full Length protein, expressed in Escherichia coli, with >95%, suitable for SDS-PAGE, WB.

View Alternative Names

GCSL, LAD, PHE3, DLD, Dihydrolipoamide dehydrogenase, Glycine cleavage system L protein

2 Images
Western blot - Recombinant Human Lipoamide Dehydrogenase protein (AB93679)
  • WB

Unknown

Western blot - Recombinant Human Lipoamide Dehydrogenase protein (AB93679)

All lanes:

Anti-Lipoamide Dehydrogenase antibody (<a href='/en-us/products/unavailable/lipoamide-dehydrogenase-antibody-ab85930'>ab85930</a>) at 1 µg/mL

All lanes:

Western blot - Recombinant Human Lipoamide Dehydrogenase protein (ab93679) at 0.01 µg

Secondary

All lanes:

Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) (ab65484) at 1/5000 dilution

true

Exposure time: 2min

SDS-PAGE - Recombinant Human Lipoamide Dehydrogenase protein (AB93679)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant Human Lipoamide Dehydrogenase protein (AB93679)

3ug by SDS-PAGE under reducing conditions and visualized by coomassie blue stain.

Key facts

Purity

>95% SDS-PAGE

Expression system

Escherichia coli

Tags

Tag free

Applications

WB, SDS-PAGE

applications

Biologically active

Yes

Biological activity

Specific activity is > 200 units/mg, one unit will reduce 1.0 umole of DL-lipoamide to DL-dihydrolipoamide per minute at pH 6.5 at 25C.

Accession

P09622

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 8 Constituents: 10% Glycerol (glycerin, glycerine), 0.58% Sodium chloride, 0.316% Tris HCl, 0.0154% (R*,R*)-1,4-Dimercaptobutan-2,3-diol

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p>ab93679 can be used as a WB positive control in conjunction with <a href='/en-us/products/unavailable/lipoamide-dehydrogenase-antibody-ab85930'>ab85930</a>.</p>" } } }
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Sequence info

[{"sequence":"","proteinLength":"Full Length","predictedMolecularWeight":null,"actualMolecularWeight":null,"aminoAcidEnd":0,"aminoAcidStart":0,"nature":"Recombinant","expressionSystem":null,"accessionNumber":"P09622","tags":[]}]
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Specifications

Form

Liquid

Additional notes

ab93679 is purified using conventional chromatography techniques.

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General info

Function

Lipoamide dehydrogenase is a component of the glycine cleavage system as well as an E3 component of three alpha-ketoacid dehydrogenase complexes (pyruvate-, alpha-ketoglutarate-, and branched-chain amino acid-dehydrogenase complex) (PubMed : 15712224, PubMed : 16442803, PubMed : 16770810, PubMed : 17404228, PubMed : 20160912, PubMed : 20385101). The 2-oxoglutarate dehydrogenase complex is mainly active in the mitochondrion (PubMed : 29211711). A fraction of the 2-oxoglutarate dehydrogenase complex also localizes in the nucleus and is required for lysine succinylation of histones : associates with KAT2A on chromatin and provides succinyl-CoA to histone succinyltransferase KAT2A (PubMed : 29211711). In monomeric form may have additional moonlighting function as serine protease (PubMed : 17404228). Involved in the hyperactivation of spermatazoa during capacitation and in the spermatazoal acrosome reaction (By similarity).

Sequence similarities

Belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family.

Post-translational modifications

Tyrosine phosphorylated.

Subcellular localisation

Mitochondrion matrix

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Product protocols

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Target data

Lipoamide dehydrogenase is a component of the glycine cleavage system as well as an E3 component of three alpha-ketoacid dehydrogenase complexes (pyruvate-, alpha-ketoglutarate-, and branched-chain amino acid-dehydrogenase complex) (PubMed : 15712224, PubMed : 16442803, PubMed : 16770810, PubMed : 17404228, PubMed : 20160912, PubMed : 20385101). The 2-oxoglutarate dehydrogenase complex is mainly active in the mitochondrion (PubMed : 29211711). A fraction of the 2-oxoglutarate dehydrogenase complex also localizes in the nucleus and is required for lysine succinylation of histones : associates with KAT2A on chromatin and provides succinyl-CoA to histone succinyltransferase KAT2A (PubMed : 29211711). In monomeric form may have additional moonlighting function as serine protease (PubMed : 17404228). Involved in the hyperactivation of spermatazoa during capacitation and in the spermatazoal acrosome reaction (By similarity).
See full target information DLD
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