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AB115504

Recombinant Human Lipoprotein lipase

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Recombinant Human Lipoprotein lipase is a Human Full Length protein, in the 28 to 475 aa range, expressed in HEK 293 cells, with >80%, suitable for SDS-PAGE, WB.

View Alternative Names

LIPD, LPL, Lipoprotein lipase, Phospholipase A1

1 Images
SDS-PAGE - Recombinant Human Lipoprotein lipase (AB115504)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant Human Lipoprotein lipase (AB115504)

14% SDS-PAGE showing ab115504
Lane 1 : M.W. marker – 14, 21, 31, 45, 66, 97 kDa
Lane 2 : reduced and boiled sample, 5μg/lane.
Lane 3 : non-reduced and non-boiled sample, 5μg/lane.

Key facts

Purity

>80% Densitometry

Expression system

HEK 293 cells

Tags

DDDDK tag N-Terminus

Applications

WB, SDS-PAGE

applications

Biologically active

No

Accession

P06858

Animal free

No

Carrier free

No

Species

Human

Reconstitution

Reconstitute at 0.5 mg/mL in water

Storage buffer

pH: 7.5 Constituents: 0.29% Sodium chloride, 0.24% Tris buffer

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Sequence info

[{"sequence":"HVDYKDDDDKPAGADQRRDFIDIESKFALRTPEDTAEDTCHLIPGVAESVATCHFNHSSKTFMVIHGWTVTGMYESWVPKLVAALYKREPDSNVIVVDWLSRAQEHYPVSAGYTKLVGQDVARFINWMEEEFNYPLDNVHLLGYSLGAHAAGIAGSLTNKKVNRITGLDPAGPNFEYAEAPSRLSPDDADFVDVLHTFTRGSPGRSIGIQKPVGHVDIYPNGGTFQPGCNIGEAIRVIAERGLGDVDQLVKCSHERSIHLFIDSLLNEENPSKAYRCSSKEAFEKGLCLSCRKNRCNNLGYEISKVRAKRSSKMYLKTRSQMPYKVFHYQVKIHFSGTESETHTNQAFEISLYGTVAESENIPFTLPEVSTNKTYSFLIYTEVDIGELLMLKLKWKSDSYFSWSDWWSSPGFAIQKIRVKAGETQKKVIFCSREKVSHLQKGKAPAVFVKCHDKSLNKKSG","proteinLength":"Full Length","predictedMolecularWeight":"51.8 kDa","actualMolecularWeight":null,"aminoAcidEnd":475,"aminoAcidStart":28,"nature":"Recombinant","expressionSystem":"HEK 293 cells","accessionNumber":"P06858","tags":[{"tag":"DDDDK","terminus":"N-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-80°C
Appropriate long-term storage conditions
-80°C
False
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Specifications

Form

Lyophilized

Additional notes

ab115504 is filtered (0.4 µm).

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General info

Function

Key enzyme in triglyceride metabolism. Catalyzes the hydrolysis of triglycerides from circulating chylomicrons and very low density lipoproteins (VLDL), and thereby plays an important role in lipid clearance from the blood stream, lipid utilization and storage (PubMed : 11342582, PubMed : 27578112, PubMed : 8675619). Although it has both phospholipase and triglyceride lipase activities it is primarily a triglyceride lipase with low but detectable phospholipase activity (PubMed : 12032167, PubMed : 7592706). Mediates margination of triglyceride-rich lipoprotein particles in capillaries (PubMed : 24726386). Recruited to its site of action on the luminal surface of vascular endothelium by binding to GPIHBP1 and cell surface heparan sulfate proteoglycans (PubMed : 11342582, PubMed : 27811232).

Sequence similarities

Belongs to the AB hydrolase superfamily. Lipase family.

Post-translational modifications

Tyrosine nitration after lipopolysaccharide (LPS) challenge down-regulates the lipase activity.

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Product protocols

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Target data

Key enzyme in triglyceride metabolism. Catalyzes the hydrolysis of triglycerides from circulating chylomicrons and very low density lipoproteins (VLDL), and thereby plays an important role in lipid clearance from the blood stream, lipid utilization and storage (PubMed : 11342582, PubMed : 27578112, PubMed : 8675619). Although it has both phospholipase and triglyceride lipase activities it is primarily a triglyceride lipase with low but detectable phospholipase activity (PubMed : 12032167, PubMed : 7592706). Mediates margination of triglyceride-rich lipoprotein particles in capillaries (PubMed : 24726386). Recruited to its site of action on the luminal surface of vascular endothelium by binding to GPIHBP1 and cell surface heparan sulfate proteoglycans (PubMed : 11342582, PubMed : 27811232).
See full target information LPL
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