Recombinant Human Liver Arginase Protein (His tag) is a Human Full Length protein, in the 1 to 322 aa range, expressed in HEK 293, with >=95% purity, <= 0.005 EU/µg endotoxin level and suitable for HPLC, MS, SDS-PAGE.
>=95% HPLC
<= 0.005 EU/µg
HEK 293 cells
His tag C-Terminus
HPLC, MS, SDS-PAGE
No
M S A K S R T I G I I G A P F S K G Q P R G G V E E G P T V L R K A G L L E K L K E Q E C D V K D Y G D L P F A D I P N D S P F Q I V K N P R S V G K A S E Q L A G K V A E V K K N G R I S L V L G G D H S L A I G S I S G H A R V H P D L G V I W V D A H T D I N T P L T T T S G N L H G Q P V S F L L K E L K G K I P D V P G F S W V T P C I S A K D I V Y I G L R D V D P G E H Y I L K T L G I K Y F S M T E V D R L G I G K V M E E T L S Y L L G R K K R P I H L S F D V D G L D P S F T P A T G T P V V G G L T Y R E G L Y I T E E I Y K T G L L S G L D I M E V N P S L G K T P E E V T R T V N T A V A I T L A C F G L A R E G N H K P I D Y L N P P K
Application | Reactivity | Dilution info | Notes |
---|---|---|---|
Application HPLC | Reactivity Reacts | Dilution info - | Notes - |
Application MS | Reactivity Reacts | Dilution info - | Notes - |
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Key element of the urea cycle converting L-arginine to urea and L-ornithine, which is further metabolized into metabolites proline and polyamides that drive collagen synthesis and bioenergetic pathways critical for cell proliferation, respectively; the urea cycle takes place primarily in the liver and, to a lesser extent, in the kidneys.Functions in L-arginine homeostasis in nonhepatic tissues characterized by the competition between nitric oxide synthase (NOS) and arginase for the available intracellular substrate arginine. Arginine metabolism is a critical regulator of innate and adaptive immune responses. Involved in an antimicrobial effector pathway in polymorphonuclear granulocytes (PMN). Upon PMN cell death is liberated from the phagolysosome and depletes arginine in the microenvironment leading to suppressed T cell and natural killer (NK) cell proliferation and cytokine secretion (PubMed:15546957, PubMed:16709924, PubMed:19380772). In group 2 innate lymphoid cells (ILC2s) promotes acute type 2 inflammation in the lung and is involved in optimal ILC2 proliferation but not survival (By similarity). In humans, the immunological role in the monocytic/macrophage/dendritic cell (DC) lineage is unsure.
Arginase-1, Liver-type arginase, Type I arginase, ARG1
Recombinant Human Liver Arginase Protein (His tag) is a Human Full Length protein, in the 1 to 322 aa range, expressed in HEK 293, with >=95% purity, <= 0.005 EU/µg endotoxin level and suitable for HPLC, MS, SDS-PAGE.
>=95% HPLC
<= 0.005 EU/µg
HEK 293 cells
His tag C-Terminus
HPLC, MS, SDS-PAGE
No
Yes
Human
Reconstitute in PBS
pH: 7.4
Constituents: 10.26% Trehalose, 0.727% Dibasic monohydrogen potassium phosphate, 0.248% Potassium phosphate monobasic
M S A K S R T I G I I G A P F S K G Q P R G G V E E G P T V L R K A G L L E K L K E Q E C D V K D Y G D L P F A D I P N D S P F Q I V K N P R S V G K A S E Q L A G K V A E V K K N G R I S L V L G G D H S L A I G S I S G H A R V H P D L G V I W V D A H T D I N T P L T T T S G N L H G Q P V S F L L K E L K G K I P D V P G F S W V T P C I S A K D I V Y I G L R D V D P G E H Y I L K T L G I K Y F S M T E V D R L G I G K V M E E T L S Y L L G R K K R P I H L S F D V D G L D P S F T P A T G T P V V G G L T Y R E G L Y I T E E I Y K T G L L S G L D I M E V N P S L G K T P E E V T R T V N T A V A I T L A C F G L A R E G N H K P I D Y L N P P K
Full Length
36.24 kDa
36.24 kDa
1 to 322
Recombinant
His tag C-Terminus
Lyophilized
SDS-PAGE >= 95%
Key element of the urea cycle converting L-arginine to urea and L-ornithine, which is further metabolized into metabolites proline and polyamides that drive collagen synthesis and bioenergetic pathways critical for cell proliferation, respectively; the urea cycle takes place primarily in the liver and, to a lesser extent, in the kidneys.
Belongs to the arginase family.
Blue Ice
Ambient
Ambient
This supplementary information is collated from multiple sources and compiled automatically.
Arginase also known as arginase-1 (ARG1) and arginase-2 (ARG2) depending on the isoform is an important enzyme in the urea cycle. Its main function is to catalyze the hydrolysis of arginine to ornithine and urea. The molecular mass of arginase varies between the isoforms with ARG1 typically around 35 kDa. Arginase is expressed widely including in the liver kidneys and red blood cells for ARG1 whereas ARG2 is more tissue-specific found particularly in the kidneys brain and small intestine.
Arginase plays a significant role in nitrogen metabolism and has a direct impact on ammonia detoxification. Arginase functions as a homotrimer facilitating the conversion of toxic ammonia into urea which the body excretes safely. The liver is the main site of the urea cycle and where ARG1 predominantly operates while ARG2 functions in mitochondrial roles associated with regulating arginine levels in other tissues. Additionally arginase impacts nitric oxide (NO) production as it competes with nitric oxide synthase (NOS) for their common substrate L-arginine.
Arginase is an important component of the urea cycle and plays a part in arginine and proline metabolism. Through its function in these pathways arginase affects several physiological processes. It competes with nitric oxide synthase for L-arginine influencing NO production which is involved in vasodilation and immune response regulation. Arginase's function in the urea cycle connects it to other urea cycle enzymes like ornithine transcarbamylase (OTC) and carbamoyl phosphate synthetase I (CPS1).
Elevated arginase activity has links to certain vascular disorders such as hypertension due to reduced nitric oxide production. Arginase also plays a role in cancer as tumor cells often display increased arginase activity which depletes local arginine affecting T-cell function and aiding tumor immune evasion. These conditions often involve interactions with proteins like nitric oxide synthase which shares L-arginine as a substrate with arginase leading to potential therapeutic targets in managing these diseases.
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Mass determination by ESI-TOF. Predicted MW is 36243.50 Da (+/-10 Da by ESI-TOF). Observed MW is 36244.37 Da.
SDS-PAGE analysis of ab307477
HPLC analysis of ab307477
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