Recombinant Human Liver Arginase protein (Tagged) is a Human Full Length protein, in the 1 to 322 aa range, expressed in HEK 293, with >95% purity, < 1 EU/µg endotoxin level and suitable for SDS-PAGE.
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Key facts
- Purity
- >95% SDS-PAGE
- Endotoxin level
- < 1 EU/µg
- Expression system
- HEK 293 cells
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE
- Biologically active
- No
Amino acid sequence
M S A K S R T I G I I G A P F S K G Q P R G G V E E G P T V L R K A G L L E K L K E Q E C D V K D Y G D L P F A D I P N D S P F Q I V K N P R S V G K A S E Q L A G K V A E V K K N G R I S L V L G G D H S L A I G S I S G H A R V H P D L G V I W V D A H T D I N T P L T T T S G N L H G Q P V S F L L K E L K G K I P D V P G F S W V T P C I S A K D I V Y I G L R D V D P G E H Y I L K T L G I K Y F S M T E V D R L G I G K V M E E T L S Y L L G R K K R P I H L S F D V D G L D P S F T P A T G T P V V G G L T Y R E G L Y I T E E I Y K T G L L S G L D I M E V N P S L G K T P E E V T R T V N T A V A I T L A C F G L A R E G N H K P I D Y L N P P K
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Key element of the urea cycle converting L-arginine to urea and L-ornithine, which is further metabolized into metabolites proline and polyamides that drive collagen synthesis and bioenergetic pathways critical for cell proliferation, respectively; the urea cycle takes place primarily in the liver and, to a lesser extent, in the kidneys. Functions in L-arginine homeostasis in nonhepatic tissues characterized by the competition between nitric oxide synthase (NOS) and arginase for the available intracellular substrate arginine. Arginine metabolism is a critical regulator of innate and adaptive immune responses. Involved in an antimicrobial effector pathway in polymorphonuclear granulocytes (PMN). Upon PMN cell death is liberated from the phagolysosome and depletes arginine in the microenvironment leading to suppressed T cell and natural killer (NK) cell proliferation and cytokine secretion (PubMed:15546957, PubMed:16709924, PubMed:19380772). In group 2 innate lymphoid cells (ILC2s) promotes acute type 2 inflammation in the lung and is involved in optimal ILC2 proliferation but not survival (By similarity). In humans, the immunological role in the monocytic/macrophage/dendritic cell (DC) lineage is unsure.
Alternative names
Arginase-1, Liver-type arginase, Type I arginase, ARG1
Recommended products
Recombinant Human Liver Arginase protein (Tagged) is a Human Full Length protein, in the 1 to 322 aa range, expressed in HEK 293, with >95% purity, < 1 EU/µg endotoxin level and suitable for SDS-PAGE.
Key facts
- Purity
- >95% SDS-PAGE
- Endotoxin level
- < 1 EU/µg
- Expression system
- HEK 293 cells
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE
- Biologically active
- No
- Accession
- P05089-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 7.4
Constituents: 20% Glycerol (glycerin, glycerine), 2.9% Sodium chloride, 0.24% Tris
Sequence info
Amino acid sequence
- M S A K S R T I G I I G A P F S K G Q P R G G V E E G P T V L R K A G L L E K L K E Q E C D V K D Y G D L P F A D I P N D S P F Q I V K N P R S V G K A S E Q L A G K V A E V K K N G R I S L V L G G D H S L A I G S I S G H A R V H P D L G V I W V D A H T D I N T P L T T T S G N L H G Q P V S F L L K E L K G K I P D V P G F S W V T P C I S A K D I V Y I G L R D V D P G E H Y I L K T L G I K Y F S M T E V D R L G I G K V M E E T L S Y L L G R K K R P I H L S F D V D G L D P S F T P A T G T P V V G G L T Y R E G L Y I T E E I Y K T G L L S G L D I M E V N P S L G K T P E E V T R T V N T A V A I T L A C F G L A R E G N H K P I D Y L N P P K
- Accession
- P05089
- Protein length
- Full Length
- Predicted molecular weight
- 36.7 kDa
- Amino acids
- 1 to 322
- Nature
- Recombinant
- Tags
- His tag N-Terminus
Specifications
- Form
- Lyophilized
General info
- Function
Key element of the urea cycle converting L-arginine to urea and L-ornithine, which is further metabolized into metabolites proline and polyamides that drive collagen synthesis and bioenergetic pathways critical for cell proliferation, respectively; the urea cycle takes place primarily in the liver and, to a lesser extent, in the kidneys.
- Sequence similarities
Belongs to the arginase family.
Storage
- Shipped at conditions
- Ambient - Can Ship with Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
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1 product image
SDS-PAGE - Recombinant Human Liver Arginase protein (Tagged) (ab276404)
SDS-PAGE analysis of ab276404
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