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AB268367

Recombinant human Livin protein (Active)

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Recombinant human Livin protein (Active) is a Human Full Length protein, expressed in Baculovirus infected Sf9 cells, with >95%, suitable for SDS-PAGE, FuncS.

View Alternative Names

KIAP, LIVIN, MLIAP, RNF50, UNQ5800/PRO19607/PRO21344, BIRC7, Baculoviral IAP repeat-containing protein 7, Kidney inhibitor of apoptosis protein, Livin, Melanoma inhibitor of apoptosis protein, RING finger protein 50, RING-type E3 ubiquitin transferase BIRC7, ML-IAP

2 Images
Functional Studies - Recombinant human Livin protein (Active) (AB268367)
  • FuncS

Unknown

Functional Studies - Recombinant human Livin protein (Active) (AB268367)

The specific activity of ab268367 was 30 nmol/min/mg in a ubiquitination assay using wild-type ubiquitin protein as substrate.

SDS-PAGE - Recombinant human Livin protein (Active) (AB268367)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant human Livin protein (Active) (AB268367)

SDS-PAGE analysis of ab268367.

Key facts

Purity

>95% SDS-PAGE

Expression system

Baculovirus infected Sf9 cells

Tags

GST tag N-Terminus

Applications

FuncS, SDS-PAGE

applications

Biologically active

Yes

Biological activity

The specific activity of ab268367 was 30 nmol/min/mg in a ubiquitination assay using wild-type ubiquitin protein as substrate.

Accession

Q96CA5

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 7.5 Constituents: 25% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, 0.79% Tris HCl, 0.31% Glutathione, 0.004% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.003% EDTA, 0.002% PMSF

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "FuncS": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Sequence info

[{"sequence":"","proteinLength":"Full Length","predictedMolecularWeight":null,"actualMolecularWeight":null,"aminoAcidEnd":0,"aminoAcidStart":0,"nature":"Recombinant","expressionSystem":null,"accessionNumber":"Q96CA5","tags":[{"tag":"GST","terminus":"N-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Dry Ice
Appropriate short-term storage conditions
-80°C
Appropriate long-term storage conditions
-80°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
True
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Specifications

Form

Liquid

Additional notes

Affinity purified.

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General info

Function

Apoptotic regulator capable of exerting proapoptotic and anti-apoptotic activities and plays crucial roles in apoptosis, cell proliferation, and cell cycle control (PubMed : 11024045, PubMed : 11084335, PubMed : 11162435, PubMed : 16729033, PubMed : 17294084). Its anti-apoptotic activity is mediated through the inhibition of CASP3, CASP7 and CASP9, as well as by its E3 ubiquitin-protein ligase activity (PubMed : 11024045, PubMed : 16729033). As it is a weak caspase inhibitor, its anti-apoptotic activity is thought to be due to its ability to ubiquitinate DIABLO/SMAC targeting it for degradation thereby promoting cell survival (PubMed : 16729033). May contribute to caspase inhibition, by blocking the ability of DIABLO/SMAC to disrupt XIAP/BIRC4-caspase interactions (PubMed : 16729033). Protects against apoptosis induced by TNF or by chemical agents such as adriamycin, etoposide or staurosporine (PubMed : 11084335, PubMed : 11162435, PubMed : 11865055). Suppression of apoptosis is mediated by activation of MAPK8/JNK1, and possibly also of MAPK9/JNK2 (PubMed : 11865055). This activation depends on TAB1 and MAP3K7/TAK1 (PubMed : 11865055). In vitro, inhibits CASP3 and proteolytic activation of pro-CASP9 (PubMed : 11024045).. Isoform 1. Blocks staurosporine-induced apoptosis (PubMed : 11322947). Promotes natural killer (NK) cell-mediated killing (PubMed : 18034418).. Isoform 2. Blocks etoposide-induced apoptosis (PubMed : 11162435, PubMed : 11322947). Protects against natural killer (NK) cell-mediated killing (PubMed : 18034418).

Sequence similarities

Belongs to the IAP family.

Post-translational modifications

Autoubiquitinated and undergoes proteasome-mediated degradation.. The truncated protein (tLivin) not only loses its anti-apoptotic effect but also acquires a pro-apoptotic effect.

Subcellular localisation

Nucleus

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Product protocols

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Target data

Apoptotic regulator capable of exerting proapoptotic and anti-apoptotic activities and plays crucial roles in apoptosis, cell proliferation, and cell cycle control (PubMed : 11024045, PubMed : 11084335, PubMed : 11162435, PubMed : 16729033, PubMed : 17294084). Its anti-apoptotic activity is mediated through the inhibition of CASP3, CASP7 and CASP9, as well as by its E3 ubiquitin-protein ligase activity (PubMed : 11024045, PubMed : 16729033). As it is a weak caspase inhibitor, its anti-apoptotic activity is thought to be due to its ability to ubiquitinate DIABLO/SMAC targeting it for degradation thereby promoting cell survival (PubMed : 16729033). May contribute to caspase inhibition, by blocking the ability of DIABLO/SMAC to disrupt XIAP/BIRC4-caspase interactions (PubMed : 16729033). Protects against apoptosis induced by TNF or by chemical agents such as adriamycin, etoposide or staurosporine (PubMed : 11084335, PubMed : 11162435, PubMed : 11865055). Suppression of apoptosis is mediated by activation of MAPK8/JNK1, and possibly also of MAPK9/JNK2 (PubMed : 11865055). This activation depends on TAB1 and MAP3K7/TAK1 (PubMed : 11865055). In vitro, inhibits CASP3 and proteolytic activation of pro-CASP9 (PubMed : 11024045).. Isoform 1. Blocks staurosporine-induced apoptosis (PubMed : 11322947). Promotes natural killer (NK) cell-mediated killing (PubMed : 18034418).. Isoform 2. Blocks etoposide-induced apoptosis (PubMed : 11162435, PubMed : 11322947). Protects against natural killer (NK) cell-mediated killing (PubMed : 18034418).
See full target information BIRC7
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