Recombinant Human LKB1 + CAB39 + LYK5 protein (His tag N-Terminus, GST tag N-Terminus)
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Recombinant Human LKB1 + CAB39 + LYK5 protein (His tag N-Terminus, GST tag N-Terminus) is a Human Full Length protein, in the 2 to 433 aa range, expressed in Baculovirus infected insect cells, with >70%, suitable for SDS-PAGE.
View Alternative Names
LKB1, PJS, STK11, Serine/threonine-protein kinase STK11, Liver kinase B1, Renal carcinoma antigen NY-REN-19, hLKB1, MO25, CGI-66, CAB39, Calcium-binding protein 39, MO25alpha, Protein Mo25, LYK5, STRAD, STRADA, STE20-related kinase adapter protein alpha, STRAD alpha, STE20-related adapter protein, Serologically defined breast cancer antigen NY-BR-96
- SDS-PAGE
Unknown
SDS-PAGE - Recombinant Human LKB1 + CAB39 + LYK5 protein (His tag N-Terminus, GST tag N-Terminus) (AB95249)
SDS-PAGE showing ab95249 at approximately 49, 66 and 74 kDa (10μg). Smaller band represents LKB1, larger band represents CAB39 and LYK5. Lane 2 represent the MW marker ladder.
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Reactivity data
Sequence info
Properties and storage information
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Aliquoting information
Storage information
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
The LKB1-CAB39-LYK5 complex acts as an important regulator in cell polarity and metabolism. These proteins together activate AMP-activated protein kinase (AMPK) and several related kinases like NUAK1 and NUAK2 which mediate cellular responses to energy stress. LKB1’s role in this complex involves phosphorylating AMPK allowing cells to maintain balance under energetic stress and by stimulating energy production while inhibiting energy-consuming processes. The complex by influencing such key processes impacts cell growth and metabolism regulation.
Pathways
The LKB1-CAB39-LYK5 complex significantly contributes to the AMPK signaling and mTOR pathways. LKB1 activates AMPK which then inhibits the mammalian target of rapamycin (mTOR) pathway important for regulating cell growth and survival in nutrient-scarce conditions. LKB1 and the complex interact with various proteins including mTOR and RAPTOR in these pathways establishing a critical link between nutrient sensing and cellular metabolism control. The pathways play significant roles in cellular energy homeostasis and lead to changes in the transcription of metabolic genes.
Specifications
Form
Liquid
Additional notes
Affinity purified.
General info
Function
Tumor suppressor serine/threonine-protein kinase that controls the activity of AMP-activated protein kinase (AMPK) family members, thereby playing a role in various processes such as cell metabolism, cell polarity, apoptosis and DNA damage response. Acts by phosphorylating the T-loop of AMPK family proteins, thus promoting their activity : phosphorylates PRKAA1, PRKAA2, BRSK1, BRSK2, MARK1, MARK2, MARK3, MARK4, NUAK1, NUAK2, SIK1, SIK2, SIK3 and SNRK but not MELK. Also phosphorylates non-AMPK family proteins such as STRADA, PTEN and possibly p53/TP53. Acts as a key upstream regulator of AMPK by mediating phosphorylation and activation of AMPK catalytic subunits PRKAA1 and PRKAA2 and thereby regulates processes including : inhibition of signaling pathways that promote cell growth and proliferation when energy levels are low, glucose homeostasis in liver, activation of autophagy when cells undergo nutrient deprivation, and B-cell differentiation in the germinal center in response to DNA damage. Also acts as a regulator of cellular polarity by remodeling the actin cytoskeleton. Required for cortical neuron polarization by mediating phosphorylation and activation of BRSK1 and BRSK2, leading to axon initiation and specification. Involved in DNA damage response : interacts with p53/TP53 and recruited to the CDKN1A/WAF1 promoter to participate in transcription activation. Able to phosphorylate p53/TP53; the relevance of such result in vivo is however unclear and phosphorylation may be indirect and mediated by downstream STK11/LKB1 kinase NUAK1. Also acts as a mediator of p53/TP53-dependent apoptosis via interaction with p53/TP53 : translocates to the mitochondrion during apoptosis and regulates p53/TP53-dependent apoptosis pathways. Regulates UV radiation-induced DNA damage response mediated by CDKN1A. In association with NUAK1, phosphorylates CDKN1A in response to UV radiation and contributes to its degradation which is necessary for optimal DNA repair (PubMed : 25329316).. Isoform 2. Has a role in spermiogenesis.
Sequence similarities
Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. LKB1 subfamily.
Post-translational modifications
Phosphorylated by ATM at Thr-363 following ionizing radiation (IR). Phosphorylation at Ser-428 by RPS6KA1 and/or some PKA is required to inhibit cell growth. Phosphorylation at Ser-428 is also required during neuronal polarization to mediate phosphorylation of BRSK1 and BRSK2 (By similarity). Phosphorylation by PKC/PRKCZ at Ser-399 in isoform 2 promotes metformin (or peroxynitrite)-induced nuclear export of STK11 and activation of AMPK. UV radiation-induced phosphorylation at Thr-363 mediates CDKN1A degradation (By similarity).. Acetylated. Deacetylation at Lys-48 enhances cytoplasmic localization and kinase activity in vitro.
Subcellular localisation
Nucleus
Target data
Additional targets
Product promise
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