Recombinant Human LONP1/Lon protein is a Human Full Length protein, in the 1 to 959 aa range, expressed in Wheat germ and suitable for ELISA, WB.
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Key facts
- Expression system
- Wheat germ
- Tags
- GST tag N-Terminus
- Applications
- ELISA, WB
- Biologically active
- No
Amino acid sequence
M A A S T G Y V R L W G A A R C W V L R R P M L A A A G G R V P T A A G A W L L R G Q R T C D A S P P W A L W G R G P A I G G Q W R G F W E A S S R G G G A F S G G E D A S E G G A E E G A G G A G G S A G A G E G P V I T A L T P M T I P D V F P H L P L I A I T R N P V F P R F I K I I E V K N K K L V E L L R R K V R L A Q P Y V G V F L K R D D S N E S D V V E S L D E I Y H T G T F A Q I H E M Q D L G D K L R M I V M G H R R V H I S R Q L E V E P E E P E A E N K H K P R R K S K R G K K E A E D E L S A R H P A E L A M E P T P E L P A E V L M V E V E N V V H E D F Q V T E E V K A L T A E I V K T I R D I I A L N P L Y R E S V L Q M M Q A G Q R V V D N P I Y L S D M G A A L T G A E S H E L Q D V L E E T N I P K R L Y K A L S L L K K E F E L S K L Q Q R L G R E V E E K I K Q T H R K Y L L Q E Q L K I I K K E L G L E K D D K D A I E E K F R E R L K E L V V P K H V M D V V D E E L S K L G L L D N H S S E F N V T R N Y L D W L T S I P W G K Y S N E N L D L A R A Q A V L E E D H Y G M E D V K K R I L E F I A V S Q L R G S T Q G K I L C F Y G P P G V G K T S I A R S I A R A L N R E Y F R F S V G G M T D V A E I K G H R R T Y V G A M P G K I I Q C L K K T K T E N P L I L I D E V D K I G R G Y Q G D P S S A L L E L L D P E Q N A N F L D H Y L D V P V D L S K V L F I C T A N V T D T I P E P L R D R M E M I N V S G Y V A Q E K L A I A E R Y L V P Q A R A L C G L D E S K A K L S S D V L T L L I K Q Y C R E S G V R N L Q K Q V E K V L R K S A Y K I V S G E A E S V E V T P E N L Q D F V G K P V F T V E R M Y D V T P P G V V M G L A W T A M G G S T L F V E T S L R R P Q D K D A K G D K D G S L E V T G Q L G E V M K E S A R I A Y T F A R A F L M Q H A P A N D Y L V T S H I H L H V P E G A T P K D G P S A G C T I V T A L L S L A M G R P V R Q N L A M T G E V S L T G K I L P V G G I K E K T I A A K R A G V T C I V L P A E N K K D F Y D L A A F I T E G L E V H F V E H Y R E I F D I A F P D E Q A E A L A V E R
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application ELISA | Reactivity Reacts | Dilution info - | Notes - |
Application WB | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
ATP-dependent serine protease that mediates the selective degradation of misfolded, unassembled or oxidatively damaged polypeptides as well as certain short-lived regulatory proteins in the mitochondrial matrix (PubMed:12198491, PubMed:15870080, PubMed:17579211, PubMed:37327776, PubMed:8248235). Endogenous substrates include mitochondrial steroidogenic acute regulatory (StAR) protein, DELE1, helicase Twinkle (TWNK) and the large ribosomal subunit protein MRPL32/bL32m (PubMed:17579211, PubMed:28377575, PubMed:37327776). MRPL32/bL32m is protected from degradation by LONP1 when it is bound to a nucleic acid (RNA), but TWNK is not (PubMed:17579211, PubMed:28377575). May also have a chaperone function in the assembly of inner membrane protein complexes (By similarity). Participates in the regulation of mitochondrial gene expression and in the maintenance of the integrity of the mitochondrial genome (PubMed:17420247). Binds to mitochondrial promoters and RNA in a single-stranded, site-specific, and strand-specific manner (PubMed:17420247). May regulate mitochondrial DNA replication and/or gene expression using site-specific, single-stranded DNA binding to target the degradation of regulatory proteins binding to adjacent sites in mitochondrial promoters (PubMed:14739292, PubMed:17420247).
Alternative names
PRSS15, LONP1, LONHs, Lon protease-like protein, Mitochondrial ATP-dependent protease Lon, Serine protease 15, LONP
Recommended products
Recombinant Human LONP1/Lon protein is a Human Full Length protein, in the 1 to 959 aa range, expressed in Wheat germ and suitable for ELISA, WB.
Key facts
- Expression system
- Wheat germ
- Tags
- GST tag N-Terminus
- Applications
- ELISA, WB
- Biologically active
- No
- Accession
- P36776-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 8
Constituents: 0.79% Tris HCl, 0.31% Glutathione
Sequence info
Amino acid sequence
- M A A S T G Y V R L W G A A R C W V L R R P M L A A A G G R V P T A A G A W L L R G Q R T C D A S P P W A L W G R G P A I G G Q W R G F W E A S S R G G G A F S G G E D A S E G G A E E G A G G A G G S A G A G E G P V I T A L T P M T I P D V F P H L P L I A I T R N P V F P R F I K I I E V K N K K L V E L L R R K V R L A Q P Y V G V F L K R D D S N E S D V V E S L D E I Y H T G T F A Q I H E M Q D L G D K L R M I V M G H R R V H I S R Q L E V E P E E P E A E N K H K P R R K S K R G K K E A E D E L S A R H P A E L A M E P T P E L P A E V L M V E V E N V V H E D F Q V T E E V K A L T A E I V K T I R D I I A L N P L Y R E S V L Q M M Q A G Q R V V D N P I Y L S D M G A A L T G A E S H E L Q D V L E E T N I P K R L Y K A L S L L K K E F E L S K L Q Q R L G R E V E E K I K Q T H R K Y L L Q E Q L K I I K K E L G L E K D D K D A I E E K F R E R L K E L V V P K H V M D V V D E E L S K L G L L D N H S S E F N V T R N Y L D W L T S I P W G K Y S N E N L D L A R A Q A V L E E D H Y G M E D V K K R I L E F I A V S Q L R G S T Q G K I L C F Y G P P G V G K T S I A R S I A R A L N R E Y F R F S V G G M T D V A E I K G H R R T Y V G A M P G K I I Q C L K K T K T E N P L I L I D E V D K I G R G Y Q G D P S S A L L E L L D P E Q N A N F L D H Y L D V P V D L S K V L F I C T A N V T D T I P E P L R D R M E M I N V S G Y V A Q E K L A I A E R Y L V P Q A R A L C G L D E S K A K L S S D V L T L L I K Q Y C R E S G V R N L Q K Q V E K V L R K S A Y K I V S G E A E S V E V T P E N L Q D F V G K P V F T V E R M Y D V T P P G V V M G L A W T A M G G S T L F V E T S L R R P Q D K D A K G D K D G S L E V T G Q L G E V M K E S A R I A Y T F A R A F L M Q H A P A N D Y L V T S H I H L H V P E G A T P K D G P S A G C T I V T A L L S L A M G R P V R Q N L A M T G E V S L T G K I L P V G G I K E K T I A A K R A G V T C I V L P A E N K K D F Y D L A A F I T E G L E V H F V E H Y R E I F D I A F P D E Q A E A L A V E R
- Accession
- P36776
- Protein length
- Full Length
- Amino acids
- 1 to 959
- Nature
- Recombinant
- Tags
- GST tag N-Terminus
Specifications
- Form
- Liquid
General info
- Function
ATP-dependent serine protease that mediates the selective degradation of misfolded, unassembled or oxidatively damaged polypeptides as well as certain short-lived regulatory proteins in the mitochondrial matrix (PubMed:12198491, PubMed:15870080, PubMed:17579211, PubMed:37327776, PubMed:8248235). Endogenous substrates include mitochondrial steroidogenic acute regulatory (StAR) protein, DELE1, helicase Twinkle (TWNK) and the large ribosomal subunit protein MRPL32/bL32m (PubMed:17579211, PubMed:28377575, PubMed:37327776). MRPL32/bL32m is protected from degradation by LONP1 when it is bound to a nucleic acid (RNA), but TWNK is not (PubMed:17579211, PubMed:28377575). May also have a chaperone function in the assembly of inner membrane protein complexes (By similarity). Participates in the regulation of mitochondrial gene expression and in the maintenance of the integrity of the mitochondrial genome (PubMed:17420247). Binds to mitochondrial promoters and RNA in a single-stranded, site-specific, and strand-specific manner (PubMed:17420247). May regulate mitochondrial DNA replication and/or gene expression using site-specific, single-stranded DNA binding to target the degradation of regulatory proteins binding to adjacent sites in mitochondrial promoters (PubMed:14739292, PubMed:17420247).
- Sequence similarities
Belongs to the peptidase S16 family.
- Subcellular localisation
- Mitochondrion matrix
Storage
- Shipped at conditions
- Dry Ice
- Appropriate short-term storage conditions
- -80°C
- Appropriate long-term storage conditions
- -80°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
This product was previously labelled as LONP1.
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1 product image
SDS-PAGE - Recombinant Human LONP1/Lon protein (ab160451)
ab160451 on a 12.5% SDS-PAGE stained with Coomassie Blue.
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