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Recombinant Human LOXL3 protein (Tagged) is a Human Fragment protein, in the 526 to 753 aa range, expressed in Baculovirus infected Sf9, with >90% purity and suitable for WB.

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Images

SDS-PAGE - Recombinant Human LOXL3 protein (Tagged) (AB174063), expandable thumbnail

Key facts

Purity

>90% SDS-PAGE

Expression system

Baculovirus infected Sf9 cells

Tags

GST tag N-Terminus

Applications

WB

Biologically active

No

Amino acid sequence

E T A S D L L L H S A L V Q E T A Y I E D R P L H M L Y C A A E E N C L A S S A R S A N W P Y G H R R L L R F S S Q I H N L G R A D F R P K A G R H S W V W H E C H G H Y H S M D I F T H Y D I L T P N G T K V A E G H K A S F C L E D T E C Q E D V S K R Y E C A N F G E Q G I T V G C W D L Y R H D I D C Q W I D I T D V K P G N Y I L Q V V I N P N F E V A E S D F T N N A M K C N C K Y D G H R I W V H N C H I G D A F S E E A N R R F E R Y P G Q T S N Q I I

Reactivity data

Application

WB

Reactivity

Reacts

Dilution info

-

Notes

-

Target data

Function

Protein-lysine 6-oxidase that mediates the oxidation of peptidyl lysine residues to allysine in target proteins (PubMed:17018530, PubMed:28065600). Catalyzes the post-translational oxidative deamination of peptidyl lysine residues in precursors of elastin and different types of collagens, a prerequisite in the formation of cross-links between collagens and elastin (PubMed:17018530). Required for somite boundary formation by catalyzing oxidation of fibronectin (FN1), enhancing integrin signaling in myofibers and their adhesion to the myotendinous junction (MTJ) (By similarity). Acts as a regulator of inflammatory response by inhibiting differentiation of naive CD4(+) T-cells into T-helper Th17 or regulatory T-cells (Treg): acts by interacting with STAT3 in the nucleus and catalyzing both deacetylation and oxidation of lysine residues on STAT3, leading to disrupt STAT3 dimerization and inhibit STAT3 transcription activity (PubMed:28065600). Oxidation of lysine residues to allysine on STAT3 preferentially takes place on lysine residues that are acetylated (PubMed:28065600). Also able to catalyze deacetylation of lysine residues on STAT3 (PubMed:28065600).Isoform 1Shows protein-lysine 6-oxidase activity toward elastin and different types of collagens, with the highest activity toward collagen type VIII (PubMed:17018530).Isoform 2Shows protein-lysine 6-oxidase activity toward elastin and different types of collagens, with the highest activity toward collagen type IV (PubMed:17018530).

Alternative names

Recommended products

Recombinant Human LOXL3 protein (Tagged) is a Human Fragment protein, in the 526 to 753 aa range, expressed in Baculovirus infected Sf9, with >90% purity and suitable for WB.

Key facts

Purity

>90% SDS-PAGE

Expression system

Baculovirus infected Sf9 cells

Applications

WB

Accession
P58215-1
Animal free

No

Species

Human

Concentration
Loading...
Storage buffer

pH: 7.5
Constituents: 25% Glycerol (glycerin, glycerine), 0.79% Tris HCl, 0.31% Glutathione, 0.29% Sodium chloride, 0.004% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.003% EDTA, 0.002% PMSF

Sequence info

Amino acid sequence

E T A S D L L L H S A L V Q E T A Y I E D R P L H M L Y C A A E E N C L A S S A R S A N W P Y G H R R L L R F S S Q I H N L G R A D F R P K A G R H S W V W H E C H G H Y H S M D I F T H Y D I L T P N G T K V A E G H K A S F C L E D T E C Q E D V S K R Y E C A N F G E Q G I T V G C W D L Y R H D I D C Q W I D I T D V K P G N Y I L Q V V I N P N F E V A E S D F T N N A M K C N C K Y D G H R I W V H N C H I G D A F S E E A N R R F E R Y P G Q T S N Q I I

Accession

P58215

Protein length

Fragment

Predicted molecular weight

54 kDa

Amino acids

526 to 753

Nature

Recombinant

Tags

GST tag N-Terminus

Specifications

Form

Liquid

Additional notes

Affinity purified.

General info

Function

Protein-lysine 6-oxidase that mediates the oxidation of peptidyl lysine residues to allysine in target proteins (PubMed:17018530, PubMed:28065600). Catalyzes the post-translational oxidative deamination of peptidyl lysine residues in precursors of elastin and different types of collagens, a prerequisite in the formation of cross-links between collagens and elastin (PubMed:17018530). Required for somite boundary formation by catalyzing oxidation of fibronectin (FN1), enhancing integrin signaling in myofibers and their adhesion to the myotendinous junction (MTJ) (By similarity). Acts as a regulator of inflammatory response by inhibiting differentiation of naive CD4(+) T-cells into T-helper Th17 or regulatory T-cells (Treg): acts by interacting with STAT3 in the nucleus and catalyzing both deacetylation and oxidation of lysine residues on STAT3, leading to disrupt STAT3 dimerization and inhibit STAT3 transcription activity (PubMed:28065600). Oxidation of lysine residues to allysine on STAT3 preferentially takes place on lysine residues that are acetylated (PubMed:28065600). Also able to catalyze deacetylation of lysine residues on STAT3 (PubMed:28065600).

Sequence similarities

Belongs to the lysyl oxidase family.

Post-translational modifications

The lysine tyrosylquinone cross-link (LTQ) is generated by condensation of the epsilon-amino group of a lysine with a topaquinone produced by oxidation of tyrosine.

Subcellular localisation

Nucleus

Storage

Shipped at conditions

Dry Ice

Appropriate short-term storage conditions

-80°C

Appropriate long-term storage conditions

-80°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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1 product image

  • SDS-PAGE - Recombinant Human LOXL3 protein (Tagged) (ab174063), expandable thumbnail

    SDS-PAGE - Recombinant Human LOXL3 protein (Tagged) (ab174063)

    SDS-PAGE showing ab174063

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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