Recombinant Human LPLA2 protein (GST tag N-Terminus)
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Recombinant Human LPLA2 protein (GST tag N-Terminus) is a Human Fragment protein, in the 314 to 412 aa range, expressed in Wheat germ, suitable for ELISA, WB.
View Alternative Names
LYPLA3, UNQ341/PRO540, PLA2G15, Lysosomal phospholipase A and acyltransferase, 1-O-acylceramide synthase, LCAT-like lysophospholipase, Lysophospholipase 3, Lysosomal phospholipase A2, Phospholipase A2 group XV, ACS, LLPL, LPLA2
- SDS-PAGE
Unknown
SDS-PAGE - Recombinant Human LPLA2 protein (GST tag N-Terminus) (AB161739)
ab161739 on a 12.5% SDS-PAGE stained with Coomassie Blue.
Complementary Products
Reactivity data
Sequence info
Properties and storage information
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Aliquoting information
Storage information
Specifications
Form
Liquid
General info
Function
Has dual calcium-independent phospholipase and O-acyltransferase activities with a potential role in glycerophospholipid homeostasis and remodeling of acyl groups of lipophilic alcohols present in acidic cellular compartments (PubMed : 10092508, PubMed : 11790796, PubMed : 20410020, PubMed : 23958596, PubMed : 25727495, PubMed : 29724779). Catalyzes hydrolysis of the ester bond of the fatty acyl group attached at sn-1 or sn-2 position of phospholipids (phospholipase A1 or A2 activity) and transfer it to the hydroxyl group at the first carbon of lipophilic alcohols (O-acyltransferase activity) (PubMed : 10092508, PubMed : 11790796, PubMed : 20410020, PubMed : 23958596, PubMed : 25727495, PubMed : 29724779, PubMed : 36823305). Among preferred fatty acyl donors are phosphatidylcholines, phosphatidylethanolamines, phosphatidylglycerols and phosphatidylserines (PubMed : 29724779, PubMed : 36823305). Favors sn-2 over sn-1 deacylation of unsaturated fatty acyl groups of phosphatidylcholines, phosphatidylethanolamines, and phosphatidylglycerols (PubMed : 36823305). Among preferred fatty acyl acceptors are natural lipophilic alcohols including short-chain ceramide N-acetyl-sphingosine (C2 ceramide), alkylacylglycerols, monoacylglycerols, and acylethanolamides such as anandamide and oleoylethanolamide (PubMed : 29724779). Selectively hydrolyzes the sn-1 fatty acyl group of truncated oxidized phospholipids and may play a role in detoxification of reactive oxidized phospholipids during oxidative stress (PubMed : 30830753). Required for normal phospholipid degradation in alveolar macrophages with potential implications in the clearance of pulmonary surfactant, which is mainly composed of dipalmitoylphosphatidylcholine (1,2-dihexadecanoyl-sn-glycero-3-phosphocholine) (By similarity). Involved in the first step of bis(monoacylglycero)phosphate (BMP) de novo synthesis from phosphatidylglycerol (1,2-diacyl-sn-glycero-3-phospho-(1'-sn-glycerol), PG) (PubMed : 36823305). BMP is an important player in cargo sorting and degradation, regulation of cellular cholesterol levels and intercellular communication (PubMed : 36823305). At neutral pH, hydrolyzes the sn-1 fatty acyl group of the lysophosphatidylcholines (PubMed : 10092508).
Sequence similarities
Belongs to the AB hydrolase superfamily. Lipase family.
Post-translational modifications
N-glycosylated (PubMed:11790796, PubMed:23958596). N-glycosylation is important for maturation of the enzyme and normal subcellular location (PubMed:23958596).
Subcellular localisation
Lysosome
Target data
Product promise
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