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AB268732

Recombinant human LRRK1 protein (Active)

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Recombinant human LRRK1 protein (Active) is a Human Fragment protein, in the 622 to 1610 aa range, expressed in Baculovirus infected Sf9 cells, with >70%, suitable for SDS-PAGE, FuncS.

View Alternative Names

KIAA1790, LRRK1, Leucine-rich repeat serine/threonine-protein kinase 1

2 Images
Functional Studies - Recombinant human LRRK1 protein (Active) (AB268732)
  • FuncS

Supplier Data

Functional Studies - Recombinant human LRRK1 protein (Active) (AB268732)

The specific activity of ab268732 was 0.21 nmol/min/mg in a kinase assay using LRRKtide (RLGRDKYKTLRQIRQ) as substrate.

SDS-PAGE - Recombinant human LRRK1 protein (Active) (AB268732)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant human LRRK1 protein (Active) (AB268732)

SDS-PAGE analysis of ab268732.

Key facts

Purity

>70% SDS-PAGE

Affinity purified.

Expression system

Baculovirus infected Sf9 cells

Tags

GST tag N-Terminus

Applications

FuncS, SDS-PAGE

applications

Biologically active

Yes

Biological activity

The specific activity of ab268732 was 0.21 nmol/min/mg in a kinase assay using LRRKtide (RLGRDKYKTLRQIRQ) as substrate.

Accession

Q38SD2

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 7.5 Constituents: 25% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, 0.79% Tris HCl, 0.31% Glutathione, 0.004% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.003% EDTA, 0.002% PMSF

storage-buffer

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Complementary Products

Primary Antibodies

AB228666

Anti-LRRK1 antibody

Western blot - Anti-LRRK1 antibody (AB228666)

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Cell Lines & Lysates

AB264726

Human LRRK1 knockout HeLa cell line

Sanger Sequencing - Human LRRK1 knockout HeLa cell line (AB264726)

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Cell Lines & Lysates

AB255402

Human LAMP2 knockout HeLa cell line

Advanced Validation

Immunocytochemistry/ Immunofluorescence - Human LAMP2 knockout HeLa cell line (AB255402)

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Cell Lines & Lysates

AB265976

Human HTT (Huntingtin) knockout HeLa cell line

Advanced Validation

Immunocytochemistry/ Immunofluorescence - Human HTT (Huntingtin) knockout HeLa cell line (AB265976)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "FuncS": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Sequence info

[{"linker":null,"sequence":"MLSYLRAQLRKAEKCKLMKMIIVGPPRQGKSTLLEILQTGRAPQVVHGEATIRTTKWELQRPAGSRAKVESVEFNVWDIGGPASMATVNQCFFTDKALYVVVWNLALGEEAVANLQFWLLNIEAKAPNAVVLVVGTHLDLIEAKFRVERIATLRAYVLALCRSPSGSRATGFPDITFKHLHEISCKSLEGQEGLRQLIFHVTCSMKDVGSTIGCQRLAGRLIPRSYLSLQEAVLAEQQRRSRDDDVQYLTDRQLEQLVEQTPDNDIKDYEDLQSAISFLIETGTLLHFPDTSHGLRNLYFLDPIWLSECLQRIFNIKGSRSVAKNGVIRAEDLRMLLVGTGFTQQTEEQYFQFLAKFEIALPVANDSYLLPHLLPSKPGLDTHGMRHPTANTIQRVFKMSFVPVGFWQRFIARMLISLAEMDLQLFENKKNTKSRNRKVTIYSFTGNQRNRCSTFRVKRNQTIYWQEGLLVTFDGGYLSVESSDVNWKKKKSGGMKIVCQSEVRDFSAMAFITDHVNSLIDQWFPALTATESDGTPLMEQYVPCPVCETAWAQHTDPSEKSEDVQYFDMEDCVLTAIERDFISCPRHPDLPVPLQELVPELFMTDFPARLFLENSKLEHSEDEGSVLGQGGSGTVIYRARYQGQPVAVKRFHIKKFKNFANVPADTMLRHLRATDAMKNFSEFRQEASMLHALQHPCIVALIGISIHPLCFALELAPLSSLNTVLSENARDSSFIPLGHMLTQKIAYQIASGLAYLHKKNIIFCDLKSDNILVWSLDVKEHINIKLSDYGISRQSFHEGALGVEGTPGYQAPEIRPRIVYDEKVDMFSYGMVLYELLSGQRPALGHHQLQIAKKLSKGIRPVLGQPEEVQFRRLQALMMECWDTKPEKRPLALSVVSQMKDPTFATFMYELCCGKQTAFFSSQGQEYTVVFWDGKEESRNYTVVNTEKGLMEVQRMCCPGMKVSCQLQVQRSLWTATEDQKIYIYTLKG","proteinLength":"Fragment","predictedMolecularWeight":null,"actualMolecularWeight":null,"aminoAcidEnd":1610,"aminoAcidStart":622,"nature":"Recombinant","expressionSystem":"Baculovirus infected Sf9 cells","accessionNumber":"Q38SD2","tags":[{"tag":"GST","terminus":"N-Terminus"}]}]
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification
Shipped at conditions
Dry Ice
Appropriate short-term storage conditions
-80°C
Appropriate long-term storage conditions
-80°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
True
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

LRRK1 also known as leucine-rich repeat kinase 1 is an important protein with a molecular mass of approximately 273 kDa. This protein belongs to the family of serine/threonine kinases and functions mechanically by phosphorylating other proteins a process critical for regulating various cellular processes. It is highly expressed in bone tissues with lower levels found in the liver and brain. LRRK1 also contains a GTPase domain which contributes to its kinase activity and is sometimes studied alongside its close relative LRRK2 due to structural similarities.
Biological function summary

LRRK1 plays a significant role in bone homeostasis and remodeling contributing to skeletal development and maintenance. LRRK1 does not form part of a larger complex but influences pathways involved in cytoskeletal dynamics and cellular motility. This protein is essential for osteoclast function where its kinase activity aids in the breakdown of bone matrix. Understanding its role in bone turnover helps researchers investigate potential therapeutic targets for bone-related conditions.

Pathways

LRRK1 is involved in signaling pathways related to bone resorption and remodeling. It participates in the RANK/RANKL/OPG pathway essential for osteoclast differentiation and activation. Additionally it interacts with proteins such as c-Src and Cbl which are critical in bone matrix dissolution and cellular adhesion processes. These interactions highlight LRRK1’s pivotal role in maintaining skeletal integrity through regulated bone degradation.

Research indicates that LRRK1 can impact conditions such as osteoporosis and Paget's disease of bone. Osteoporosis involves decreased bone mass and increased fracture risk which relates to dysregulated osteoclast activity in which LRRK1 may play a part. In Paget's disease abnormal bone remodeling processes occur possibly involving dysfunctions in LRRK1 signaling. The interaction of LRRK1 with LRRK2 is also relevant in this context as both enzymes share mechanistic features that might influence bone-related pathologies.
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General info

Function

Serine/threonine-protein kinase which phosphorylates RAB proteins involved in intracellular trafficking (PubMed : 36040231). Phosphorylates RAB7A; this activity is dependent on protein kinase C (PKC) activation (PubMed : 36040231, PubMed : 37558661, PubMed : 37857821). Plays a role in the negative regulation of bone mass, acting through the maturation of osteoclasts (By similarity).

Sequence similarities

Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family. ROCO subfamily.

Post-translational modifications

Autophosphorylated (PubMed:16243488). Autophosphorylation is inhibited in its dimeric state (PubMed:22952686, PubMed:37857821). Phosphorylated by protein kinase C isozymes PRKCA, PRKCB, PRKCG, PRKCE, PRKCZ and PRKCT at Thr-1061, Ser-1064, Ser-1074 and Thr-1075 (PubMed:36040231). Phosphorylation at these Ser-1064, Ser-1074 and Thr-1075 activates the kinase activity of LRRK1 to phosphorylate RAB7A (PubMed:36040231).

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Product protocols

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Target data

Serine/threonine-protein kinase which phosphorylates RAB proteins involved in intracellular trafficking (PubMed : 36040231). Phosphorylates RAB7A; this activity is dependent on protein kinase C (PKC) activation (PubMed : 36040231, PubMed : 37558661, PubMed : 37857821). Plays a role in the negative regulation of bone mass, acting through the maturation of osteoclasts (By similarity).
See full target information LRRK1
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