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AB271603

Recombinant human LSD2 / AOF1 protein (Active)

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Recombinant human LSD2 / AOF1 protein (Active) is a Human Full Length protein, in the 2 to 822 aa range, expressed in Baculovirus infected Sf9 cells, with >90%, suitable for SDS-PAGE, FuncS.

View Alternative Names

AOF1, C6orf193, LSD2, KDM1B, Lysine-specific histone demethylase 2, Flavin-containing amine oxidase domain-containing protein 1, Lysine-specific histone demethylase 1B

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SDS-PAGE - Recombinant human LSD2 / AOF1 protein (Active) (AB271603)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant human LSD2 / AOF1 protein (Active) (AB271603)

Specific activity of ab271603.

SDS-PAGE - Recombinant human LSD2 / AOF1 protein (Active) (AB271603)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant human LSD2 / AOF1 protein (Active) (AB271603)

SDS-PAGE analysis of ab271603.

Key facts

Purity

>90% SDS-PAGE

Expression system

Baculovirus infected Sf9 cells

Tags

Tag free

Applications

SDS-PAGE, FuncS

applications

Biologically active

Yes

Biological activity

Active

Accession

Q8NB78

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 8 Constituents: 20% Glycerol (glycerin, glycerine), 0.64% Sodium chloride, 0.63% Tris HCl, 0.05% Glutathione, 0.04% Sorbitan monolaurate, ethoxylated, 0.02% Potassium chloride

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "FuncS": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Sequence info

[{"sequence":"","proteinLength":"Full Length","predictedMolecularWeight":"121 kDa","actualMolecularWeight":null,"aminoAcidEnd":822,"aminoAcidStart":2,"nature":"Recombinant","expressionSystem":null,"accessionNumber":"Q8NB78","tags":[]}]
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Properties and storage information

Shipped at conditions
Dry Ice
Appropriate short-term storage conditions
-80°C
Appropriate long-term storage conditions
-80°C
Storage information
Avoid freeze / thaw cycle
True
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

LSD2 also known as AOF1 is a lysine-specific demethylase enzyme with a mass of about 105 kDa. This protein mainly functions by removing methyl groups from lysine residues on histone proteins thereby affecting chromatin structure and gene expression. LSD2 is expressed in various tissues with high levels in the liver and brain. The enzyme plays a significant role in regulating gene activity influencing many biological processes.
Biological function summary

LSD2 impacts gene expression by altering the epigenetic state of chromatin. It is not part of a well-defined large multiprotein complex allowing LSD2 to directly interact with histones. Through its demethylase activity it modulates the transcriptional regulation impacting cellular differentiation and proliferation. This makes it an important factor in maintaining cellular homeostasis.

Pathways

LSD2 is involved in histone modification processes particularly through the demethylation of H3K4me1 and H3K4me2 marks. These changes are important in the regulation of gene expression patterns. LSD2 often interacts with the REST corepressor complex affecting the expression of genes involved in neural development and function. It plays a role in pathways related to cellular stress responses and metabolic regulation highlighting its importance in cellular adaptation mechanisms.

LSD2 has been implicated in cancer and neurological disorders. Alterations in its expression and activity are associated with certain types of cancers such as breast cancer where it affects tumor growth and progression by influencing gene expression patterns. In neurological contexts LSD2's dysregulation impacts neural circuitry and cognitive functions which can be linked to disorders like Alzheimer's disease. In these conditions LSD2 interacts with proteins such as SIRT1 influencing cellular processes that contribute to disease pathology.
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Specifications

Form

Liquid

Additional notes

Affinity purified.

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General info

Function

Histone demethylase that demethylates 'Lys-4' of histone H3, a specific tag for epigenetic transcriptional activation, thereby acting as a corepressor. Required for de novo DNA methylation of a subset of imprinted genes during oogenesis. Acts by oxidizing the substrate by FAD to generate the corresponding imine that is subsequently hydrolyzed. Demethylates both mono- and di-methylated 'Lys-4' of histone H3. Has no effect on tri-methylated 'Lys-4', mono-, di- or tri-methylated 'Lys-9', mono-, di- or tri-methylated 'Lys-27', mono-, di- or tri-methylated 'Lys-36' of histone H3, or on mono-, di- or tri-methylated 'Lys-20' of histone H4. Alone, it is unable to demethylate H3K4me on nucleosomes and requires the presence of GLYR1 to achieve such activity, they form a multifunctional enzyme complex that modifies transcribed chromatin and facilitates Pol II transcription through nucleosomes (PubMed : 30970244).

Sequence similarities

Belongs to the flavin monoamine oxidase family.

Subcellular localisation

Nucleus

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Product protocols

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Target data

Histone demethylase that demethylates 'Lys-4' of histone H3, a specific tag for epigenetic transcriptional activation, thereby acting as a corepressor. Required for de novo DNA methylation of a subset of imprinted genes during oogenesis. Acts by oxidizing the substrate by FAD to generate the corresponding imine that is subsequently hydrolyzed. Demethylates both mono- and di-methylated 'Lys-4' of histone H3. Has no effect on tri-methylated 'Lys-4', mono-, di- or tri-methylated 'Lys-9', mono-, di- or tri-methylated 'Lys-27', mono-, di- or tri-methylated 'Lys-36' of histone H3, or on mono-, di- or tri-methylated 'Lys-20' of histone H4. Alone, it is unable to demethylate H3K4me on nucleosomes and requires the presence of GLYR1 to achieve such activity, they form a multifunctional enzyme complex that modifies transcribed chromatin and facilitates Pol II transcription through nucleosomes (PubMed : 30970244).
See full target information KDM1B
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Complementary Products

Primary Antibodies

AB193080

Anti-LSD2 / AOF1 antibody [EPR18508]

RabMAb|Recombinant|KO Validated

Immunocytochemistry/ Immunofluorescence - Anti-LSD2 / AOF1 antibody [EPR18508] (AB193080)

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Cell Lines & Lysates

AB258016

Human KDM1B (LSD2 / AOF1) knockout HeLa cell lysate

Western blot - Human KDM1B (LSD2 / AOF1) knockout HeLa cell lysate (AB258016)

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