Recombinant Human LSECtin protein (Fc fragment) is a Human Fragment protein, in the 55 to 293 aa range, expressed in HEK 293, with >85% purity, < 1 EU/µg endotoxin level and suitable for SDS-PAGE.
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Key facts
- Purity
>85% SDS-PAGE
- Endotoxin level
< 1 EU/µg
- Expression system
HEK 293 cells
- Tags
Tag free
- Applications
SDS-PAGE
- Biologically active
No
Amino acid sequence
K A S T E R A A L L D G H D L L R T N A S K Q T A A L G A L K E E V G D C H S C C S G T Q A Q L Q T T R A E L G E A Q A K L M E Q E S A L R E L R E R V T Q G L A E A G R G R E D V R T E L F R A L E A V R L Q N N S C E P C P T S W L S F E G S C Y F F S V P K T T W A A A Q D H C A D A S A H L V I V G G L D E Q G F L T R N T R G R G Y W L G L R A V R H L G K V Q G Y Q W V D G V S L S F S H W N Q G E P N D A W G R E N C V M M L H T G L W N D A P C D S E K D G W I C E K R H N C
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Binds mannose, N-acetylglucosamine (GlcNAc) and fucose, but not galactose, in a Ca(2+)-dependent manner, in vitro.(Microbial infection) Acts as a receptor for Japanese encephalitis virus.(Microbial infection) Acts as a receptor for Ebolavirus.(Microbial infection) Acts as a receptor for SARS-CoV.(Microbial infection) Acts as a receptor for Lassa virus and Lymphocytic choriomeningitis virus glycoprotein (PubMed:22156524, PubMed:22673088).
Alternative names
C-type lectin domain family 4 member G, Liver and lymph node sinusoidal endothelial cell C-type lectin, LSECtin, CLEC4G, UNQ431/PRO792
Recommended products
Recombinant Human LSECtin protein (Fc fragment) is a Human Fragment protein, in the 55 to 293 aa range, expressed in HEK 293, with >85% purity, < 1 EU/µg endotoxin level and suitable for SDS-PAGE.
Key facts
- Purity
>85% SDS-PAGE
- Endotoxin level
< 1 EU/µg
- Expression system
HEK 293 cells
- Tags
Tag free
- Applications
SDS-PAGE
- Biologically active
No
- Accession
- Q6UXB4-1
- Animal free
No
- Species
Human
- Reconstitution
Reconstitute with sterile deionized water to a concentration of 200 µg/ml.
- Concentration
- Storage buffer
pH: 7.4
Constituents: PBS, 5% Trehalose
Sequence info
Amino acid sequence
K A S T E R A A L L D G H D L L R T N A S K Q T A A L G A L K E E V G D C H S C C S G T Q A Q L Q T T R A E L G E A Q A K L M E Q E S A L R E L R E R V T Q G L A E A G R G R E D V R T E L F R A L E A V R L Q N N S C E P C P T S W L S F E G S C Y F F S V P K T T W A A A Q D H C A D A S A H L V I V G G L D E Q G F L T R N T R G R G Y W L G L R A V R H L G K V Q G Y Q W V D G V S L S F S H W N Q G E P N D A W G R E N C V M M L H T G L W N D A P C D S E K D G W I C E K R H N C
- Accession
- Protein length
Fragment
- Predicted molecular weight
52.1 kDa
- Amino acids
55 to 293
- Nature
Recombinant
Specifications
- Form
Lyophilized
General info
- Function
Binds mannose, N-acetylglucosamine (GlcNAc) and fucose, but not galactose, in a Ca(2+)-dependent manner, in vitro.
Storage
- Shipped at conditions
Blue Ice
- Appropriate long-term storage conditions
-20°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Avoid freeze / thaw cycle
Notes
Previously labelled as CLEC4G.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
Supplementary info
This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
LSECtin also known as CLEC4G is a C-type lectin-like receptor weighing around 40 kDa. It is expressed mainly on liver sinusoidal endothelial cells (LSECs) but also noted in lymph node and placenta tissues. As a receptor LSECtin plays an important role in recognizing and binding to specific glycans on pathogens facilitating their capture and subsequent immune response.
- Biological function summary
LSECtin functions as an important player in immune recognition and clearance of pathogens. It is involved in pathogen binding through interaction with glycans aiding in the modulation of immune responses. LSECtin along with other related receptors like DC-SIGN and CD23 forms part of a complex that influences pathogen recognition. It particularly interacts with viruses like HIV and Ebola impacting how these pathogens are detected and processed by the immune system.
- Pathways
The function of LSECtin integrates into immune system pathways where it influences pathogen clearance. One notable pathway is the pathogen recognition pathway. LSECtin interacts with the liver's immune regulatory network linking with proteins like DC-SIGN that play a role in modulating the immune response. Another pathway it affects is the cell adhesion pathway which involves inflammation regulation.
- Associated diseases and disorders
LSECtin is linked to infections and immune-related liver conditions. In viral infections such as Ebola and HIV LSECtin mediates pathogen capture affecting the body's ability to handle these infections. The receptor's interaction with liver-specific proteins is noted in liver fibrosis where LSECtin and related proteins like CD23 may alter immune function and disease progression. This close relationship highlights the importance of LSECtin in both immune defense and disease pathology.
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1 product image
SDS-PAGE - Recombinant Human LSECtin protein (Fc fragment) (ab204209)
SDS-PAGE of reduced ab204209 stained overnight with Coomassie Blue. The protein migrates as 58-66 kDa in SDS-PAGE due to glycosylation.
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