JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB124544

Recombinant Human LSM2 protein (His tag N-Terminus)

Be the first to review this product! Submit a review

|

(0 Publication)

Recombinant Human LSM2 protein (His tag N-Terminus) is a Human Full Length protein, in the 1 to 95 aa range, expressed in Escherichia coli, with >90%, suitable for SDS-PAGE, Mass Spec.

View Alternative Names

C6orf28, G7B, LSM2, U6 snRNA-associated Sm-like protein LSm2, Protein G7b, Small nuclear ribonuclear protein D homolog, snRNP core Sm-like protein Sm-x5

1 Images
SDS-PAGE - Recombinant Human LSM2 protein (His tag N-Terminus) (AB124544)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant Human LSM2 protein (His tag N-Terminus) (AB124544)

15% SDS-PAGE analysis of 3 μg ab124544.

Key facts

Purity

>90% SDS-PAGE

Expression system

Escherichia coli

Tags

His tag N-Terminus

Applications

Mass Spec, SDS-PAGE

applications

Biologically active

No

Accession

Q9Y333

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 8 Constituents: 40% Glycerol (glycerin, glycerine), 1.17% Sodium chloride, 0.32% Tris HCl, 0.08% (R*,R*)-1,4-Dimercaptobutan-2,3-diol

storage-buffer

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "Mass Spec": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
style
left-column
style
left-column

Sequence info

[{"sequence":"MGSSHHHHHHSSGLVPRGSHMGSHMLFYSFFKSLVGKDVVVELKNDLSICGTLHSVDQYLNIKLTDISVTDPEKYPHMLSVKNCFIRGSVVRYVQLPADEVDTQLLQDAARKEALQQKQ","proteinLength":"Full Length","predictedMolecularWeight":"13.4 kDa","actualMolecularWeight":null,"aminoAcidEnd":95,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"Q9Y333","tags":[{"tag":"His","terminus":"N-Terminus"}]}]
style
left-column

Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

LSM2 also known as Like-Smith 2 is a protein involved in RNA processing and stabilization. It has a mass of approximately 14 kDa. This protein is mainly expressed in the cytoplasm and nucleus and its presence is critical in cells with active RNA metabolism. The structure of LSM2 enables it to participate in RNA decay and splicing pathways making it an important player in RNA regulation.
Biological function summary

Proteins coordinate complex molecular processes within cells. LSM2 is part of the Lsm1-7 complex which binds to the 3' ends of mRNA and facilitates mRNA decapping and decay. This complex supports RNA metabolism by modulating mRNA stability and is essential for proper cellular function and response to environmental changes.

Pathways

The functions of LSM2 integrate into RNA processing pathways like mRNA decay and splicing. In the mRNA decay pathway LSM2 through the Lsm1-7 complex works alongside decapping proteins to regulate mRNA turnover influencing gene expression levels. Additionally in splicing LSM2 influences the splicing machinery interacting with proteins like U6 snRNA ensuring accurate splicing of pre-mRNA.

Researchers have linked abnormalities in LSM2 function to neurodegenerative diseases such as spinal muscular atrophy. Mutations or malfunctions in LSM2 disrupt RNA processing especially mRNA decay leading to improper gene expression. These disruptions can involve proteins like SMN (Survival Motor Neuron) highlighting LSM2's role in maintaining neuron integrity and function.
style
left-column

Specifications

Form

Liquid

Additional notes

ab124544 is purified using conventional chromatography techniques.

style
left-column

General info

Function

Plays a role in pre-mRNA splicing as component of the U4/U6-U5 tri-snRNP complex that is involved in spliceosome assembly, and as component of the precatalytic spliceosome (spliceosome B complex) (PubMed : 28781166). The heptameric LSM2-8 complex binds specifically to the 3'-terminal U-tract of U6 snRNA (PubMed : 10523320).

Sequence similarities

Belongs to the snRNP Sm proteins family.

Subcellular localisation

Nucleus

style
left-column

Product protocols

style
left-column

Target data

Plays a role in pre-mRNA splicing as component of the U4/U6-U5 tri-snRNP complex that is involved in spliceosome assembly, and as component of the precatalytic spliceosome (spliceosome B complex) (PubMed : 28781166). The heptameric LSM2-8 complex binds specifically to the 3'-terminal U-tract of U6 snRNA (PubMed : 10523320).
See full target information LSM2
style
left-column
style
left-column
style
right-column

Complementary Products

Primary Antibodies

AB229316

Anti-LSM1 antibody

Immunoprecipitation - Anti-LSM1 antibody (AB229316)

  • 0
  • 0
Primary Antibodies

AB129164

Anti-LSM2 antibody [EPR7216]

RabMAb|Recombinant

Western blot - Anti-LSM2 antibody [EPR7216] (AB129164)

  • 0
  • 0
Proteins & Peptides

AB114393

Recombinant Human TRA2B/SFRS10 protein

SDS-PAGE - Recombinant Human TRA2B/SFRS10 protein (AB114393)

  • 0
  • 0
Proteins & Peptides

AB114626

Recombinant Human KPNA4 protein

SDS-PAGE - Recombinant Human KPNA4 protein (AB114626)

  • 0
  • 0

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com