Recombinant Human LSM2 protein is a Human Full Length protein, in the 1 to 95 aa range, expressed in Escherichia coli, with >90% purity and suitable for SDS-PAGE, MS.
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Key facts
- Purity
- >90% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE, MS
- Biologically active
- No
Amino acid sequence
M G S S H H H H H H S S G L V P R G S H M G S H M L F Y S F F K S L V G K D V V V E L K N D L S I C G T L H S V D Q Y L N I K L T D I S V T D P E K Y P H M L S V K N C F I R G S V V R Y V Q L P A D E V D T Q L L Q D A A R K E A L Q Q K Q
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application MS | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Plays a role in pre-mRNA splicing as component of the U4/U6-U5 tri-snRNP complex that is involved in spliceosome assembly, and as component of the precatalytic spliceosome (spliceosome B complex) (PubMed:28781166). The heptameric LSM2-8 complex binds specifically to the 3'-terminal U-tract of U6 snRNA (PubMed:10523320).
Alternative names
C6orf28, G7B, LSM2, U6 snRNA-associated Sm-like protein LSm2, Protein G7b, Small nuclear ribonuclear protein D homolog, snRNP core Sm-like protein Sm-x5
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Recombinant Human LSM2 protein is a Human Full Length protein, in the 1 to 95 aa range, expressed in Escherichia coli, with >90% purity and suitable for SDS-PAGE, MS.
Key facts
- Purity
- >90% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE, MS
- Biologically active
- No
- Accession
- Q9Y333-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 8
Constituents: 40% Glycerol (glycerin, glycerine), 1.17% Sodium chloride, 0.32% Tris HCl, 0.08% (R*,R*)-1,4-Dimercaptobutan-2,3-diol
Sequence info
Amino acid sequence
- M G S S H H H H H H S S G L V P R G S H M G S H M L F Y S F F K S L V G K D V V V E L K N D L S I C G T L H S V D Q Y L N I K L T D I S V T D P E K Y P H M L S V K N C F I R G S V V R Y V Q L P A D E V D T Q L L Q D A A R K E A L Q Q K Q
- Accession
- Q9Y333
- Protein length
- Full Length
- Predicted molecular weight
- 13.4 kDa
- Amino acids
- 1 to 95
- Nature
- Recombinant
- Tags
- His tag N-Terminus
Specifications
- Form
- Liquid
- Additional notes
ab124544 is purified using conventional chromatography techniques.
General info
- Function
Plays a role in pre-mRNA splicing as component of the U4/U6-U5 tri-snRNP complex that is involved in spliceosome assembly, and as component of the precatalytic spliceosome (spliceosome B complex) (PubMed:28781166). The heptameric LSM2-8 complex binds specifically to the 3'-terminal U-tract of U6 snRNA (PubMed:10523320).
- Sequence similarities
Belongs to the snRNP Sm proteins family.
- Subcellular localisation
- Nucleus
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
LSM2 also known as Like-Smith 2 is a protein involved in RNA processing and stabilization. It has a mass of approximately 14 kDa. This protein is mainly expressed in the cytoplasm and nucleus and its presence is critical in cells with active RNA metabolism. The structure of LSM2 enables it to participate in RNA decay and splicing pathways making it an important player in RNA regulation.
- Biological function summary
Proteins coordinate complex molecular processes within cells. LSM2 is part of the Lsm1-7 complex which binds to the 3' ends of mRNA and facilitates mRNA decapping and decay. This complex supports RNA metabolism by modulating mRNA stability and is essential for proper cellular function and response to environmental changes.
- Pathways
The functions of LSM2 integrate into RNA processing pathways like mRNA decay and splicing. In the mRNA decay pathway LSM2 through the Lsm1-7 complex works alongside decapping proteins to regulate mRNA turnover influencing gene expression levels. Additionally in splicing LSM2 influences the splicing machinery interacting with proteins like U6 snRNA ensuring accurate splicing of pre-mRNA.
- Associated diseases and disorders
Researchers have linked abnormalities in LSM2 function to neurodegenerative diseases such as spinal muscular atrophy. Mutations or malfunctions in LSM2 disrupt RNA processing especially mRNA decay leading to improper gene expression. These disruptions can involve proteins like SMN (Survival Motor Neuron) highlighting LSM2's role in maintaining neuron integrity and function.
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1 product image
SDS-PAGE - Recombinant Human LSM2 protein (ab124544)
15% SDS-PAGE analysis of 3 μg ab124544.
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