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AB101871

Recombinant Human LSM4 protein

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Recombinant Human LSM4 protein is a Human Full Length protein, in the 1 to 139 aa range, expressed in Escherichia coli, with >90%, suitable for SDS-PAGE, Mass Spec.

View Alternative Names

U6 snRNA-associated Sm-like protein LSm4, Glycine-rich protein, GRP, LSM4

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SDS-PAGE - Recombinant Human LSM4 protein (AB101871)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant Human LSM4 protein (AB101871)

15% SDS PAGE analysis of 3μg ab101871.

Key facts

Purity

>90% SDS-PAGE

Expression system

Escherichia coli

Tags

His tag N-Terminus

Applications

SDS-PAGE, Mass Spec

applications

Biologically active

No

Accession

Q9Y4Z0

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 8 Constituents: 20% Glycerol (glycerin, glycerine), 0.58% Sodium chloride, 0.316% Tris HCl, 0.0154% (R*,R*)-1,4-Dimercaptobutan-2,3-diol

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "Mass Spec": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Sequence info

[{"sequence":"MGSSHHHHHHSSGLVPRGSHMLPLSLLKTAQNHPMLVELKNGETYNGHLVSCDNWMNINLREVICTSRDGDKFWRMPECYIRGSTIKYLRIPDEIIDMVKEEVVAKGRGRGGLQQQKQQKGRGMGGAGRGVFGGRGRGGIPGTGRGQPEKKPGRQAGKQ","proteinLength":"Full Length","predictedMolecularWeight":"17.5 kDa","actualMolecularWeight":null,"aminoAcidEnd":139,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"Q9Y4Z0","tags":[{"tag":"His","terminus":"N-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

LSM4 also known as Like-Sm protein 4 is a component of the LSM protein family with a molecular mass of approximately 12 kDa. It plays a mechanical role in the formation of the LSM complex which is similar to the Sm proteins involved in RNA processing. Expression of LSM4 occurs in various tissues including the brain and immune cells indicating its diverse functional significance across different biological systems.
Biological function summary

Like-Sm protein 4 plays an important role in RNA processing particularly in mRNA splicing and degradation. It forms part of the LSm1-7 complex which associates with the U6 snRNA to regulate the splicing mechanisms necessary for proper gene expression. By ensuring accurate mRNA splicing LSM4 contributes to the maintenance of cellular function and homeostasis.

Pathways

LSM4's involvement in RNA splicing positions it within the spliceosome pathway essential for processing pre-mRNA into mature mRNA. Through this pathway it collaborates with other LSm proteins and the DEAD-box helicase DDX41. LSM4 also has a role in the RNA decay pathway where it interacts with the XRN1 protein facilitating the turnover of mRNA to control gene expression levels in cells.

Errors in LSM4 function may lead to neurological disorders such as spinal muscular atrophy. This association occurs through its interaction with the SMN protein where disruptions in RNA splicing affect motor neuron survival. LSM4 also associates with cancerous conditions as improper mRNA processing can lead to oncogene activation influencing processes like cell proliferation and apoptosis.
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Specifications

Form

Liquid

Additional notes

ab101871 was purified using conventional chromatography.

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General info

Function

Plays a role in pre-mRNA splicing as component of the U4/U6-U5 tri-snRNP complex that is involved in spliceosome assembly, and as component of the precatalytic spliceosome (spliceosome B complex) (PubMed : 28781166). The heptameric LSM2-8 complex binds specifically to the 3'-terminal U-tract of U6 snRNA (PubMed : 10523320).

Sequence similarities

Belongs to the snRNP Sm proteins family.

Subcellular localisation

Nucleus

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Plays a role in pre-mRNA splicing as component of the U4/U6-U5 tri-snRNP complex that is involved in spliceosome assembly, and as component of the precatalytic spliceosome (spliceosome B complex) (PubMed : 28781166). The heptameric LSM2-8 complex binds specifically to the 3'-terminal U-tract of U6 snRNA (PubMed : 10523320).
See full target information LSM4
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions
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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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