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Recombinant human LTA protein is a Human Full Length protein, expressed in Escherichia coli, with >=98% purity and suitable for SDS-PAGE, FuncS.

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Key facts

Purity
>=98% SDS-PAGE
Expression system
Escherichia coli
Tags
Tag free
Applications
SDS-PAGE, FuncS
Biologically active
Yes

Amino acid sequence

M L P G V G L T P S A A Q T A R Q H P K M H L A H S T L K P A A H L I G D P S K Q N S L L W R A N T D R A F L Q D G F S L S N N S L L V P T S G I Y F V Y S Q V V F S G K A Y S P K A T S S P L Y L A H E V Q L F S S Q Y P F H V P L L S S Q K M V Y P G L Q E P W L H S M Y H G A A F Q L T Q G D Q L S T H T D G I P H L V L S P S T V F F G A F A L

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Reactivity data

Application
SDS-PAGE
Reactivity
Reacts
Dilution info
-
Notes

-

Application
FuncS
Reactivity
Reacts
Dilution info
-
Notes

-

Associated Products

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Target data

Function

Cytokine that in its homotrimeric form binds to TNFRSF1A/TNFR1, TNFRSF1B/TNFBR and TNFRSF14/HVEM (PubMed:9462508). In its heterotrimeric form with LTB binds to TNFRSF3/LTBR (PubMed:24248355). Lymphotoxin is produced by lymphocytes and is cytotoxic for a wide range of tumor cells in vitro and in vivo.

Alternative names

Recommended products

Recombinant human LTA protein is a Human Full Length protein, expressed in Escherichia coli, with >=98% purity and suitable for SDS-PAGE, FuncS.

Key facts

Purity
>=98% SDS-PAGE
Expression system
Escherichia coli
Applications
SDS-PAGE, FuncS
Biological activity
The ED50, determined by the cytolysis of murine L929 cells in the presence of actinomycin D, is < 0.05 ng/ml, corresponding to a specific activity of > 2 x 107 units/mg.
Accession
P01374-1
Animal free
No
Species
Human
Reconstitution
Reconstitute with 20µl pure water.
Concentration
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Sequence info

Amino acid sequence

M L P G V G L T P S A A Q T A R Q H P K M H L A H S T L K P A A H L I G D P S K Q N S L L W R A N T D R A F L Q D G F S L S N N S L L V P T S G I Y F V Y S Q V V F S G K A Y S P K A T S S P L Y L A H E V Q L F S S Q Y P F H V P L L S S Q K M V Y P G L Q E P W L H S M Y H G A A F Q L T Q G D Q L S T H T D G I P H L V L S P S T V F F G A F A L
Accession
P01374
Protein length
Full Length
Predicted molecular weight
18.6 kDa
Nature
Recombinant

Specifications

Form
Lyophilized
Additional notes

Sterile filtered Greater than 98% pure by HPLC analyses. Endotoxin level is less than 0.1 ng per µg (1EU/µg).

General info

Function

Cytokine that in its homotrimeric form binds to TNFRSF1A/TNFR1, TNFRSF1B/TNFBR and TNFRSF14/HVEM (PubMed:9462508). In its heterotrimeric form with LTB binds to TNFRSF3/LTBR (PubMed:24248355). Lymphotoxin is produced by lymphocytes and is cytotoxic for a wide range of tumor cells in vitro and in vivo.

Sequence similarities

Belongs to the tumor necrosis factor family.

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
Up to 6 months
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C

This product is an active protein and may elicit a biological response in vivo, handle with caution.

Notes

The ED50, determined by the cytolysis of murine L929 cells in the presence of actinomycin D, is < 0.05 ng/ml, corresponding to a specific activity of > 2 x 107 units/mg.
This product was previously labelled as TNF beta

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