Recombinant Human LYAR protein is a Human Full Length protein, in the 1 to 379 aa range, expressed in Wheat germ and suitable for ELISA, WB.
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- Scientific reports 12:105682022SILAC kinase screen identifies potential MASTL substrates.Applications:Unspecified applicationReactive species:Unspecified reactive speciesKamila A Marzec,Samuel Rogers,Rachael McCloy,Benjamin L Parker,David E James,D Neil Watkins,Andrew BurgessPubMed 35732702
Key facts
- Expression system
- Wheat germ
- Tags
- GST tag N-Terminus
- Applications
- ELISA, WB
- Biologically active
- No
Amino acid sequence
M V F F T C N A C G E S V K K I Q V E K H V S V C R N C E C L S C I D C G K D F W G D D Y K N H V K C I S E D Q K Y G G K G Y E G K T H K G D I K Q Q A W I Q K I S E L I K R P N V S P K V R E L L E Q I S A F D N V P R K K A K F Q N W M K N S L K V H N E S I L D Q V W N I F S E A S N S E P V N K E Q D Q R P L H P V A N P H A E I S T K V P A S K V K D A V E Q Q G E V K K N K R E R K E E R Q K K R K R E K K E L K L E N H Q E N S R N Q K P K K R K K G Q E A D L E A G G E E V P E A N G S A G K R S K K K K Q R K D S A S E E E A R V G A G K R K R R H S E V E T D S K K K K M K L P E H P E G G E P E D D E A P A K G K F N W K G T I K A I L K Q A P D N E I T I K K L R K K V L A Q Y Y T V T D E H H R S E E E L L V I F N K K I S K N P T F K L L K D K V K L V K
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application ELISA | Reactivity Reacts | Dilution info - | Notes - |
Application WB | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Plays a role in the maintenance of the appropriate processing of 47S/45S pre-rRNA to 32S/30S pre-rRNAs and their subsequent processing to produce 18S and 28S rRNAs (PubMed:24495227). Also acts at the level of transcription regulation. Along with PRMT5, binds the gamma-globin (HBG1/HBG2) promoter and represses its expression (PubMed:25092918). In neuroblastoma cells, may also repress the expression of oxidative stress genes, including CHAC1, HMOX1, SLC7A11, ULBP1 and SNORD41 that encodes a small nucleolar RNA (PubMed:28686580). Preferentially binds to a DNA motif containing 5'-GGTTAT-3' (PubMed:25092918). Negatively regulates the antiviral innate immune response by targeting IRF3 and impairing its DNA-binding activity (PubMed:31413131). In addition, inhibits NF-kappa-B-mediated expression of pro-inflammatory cytokines (PubMed:31413131). Stimulates phagocytosis of photoreceptor outer segments by retinal pigment epithelial cells (By similarity). Prevents nucleolin/NCL self-cleavage, maintaining a normal steady-state level of NCL protein in undifferentiated embryonic stem cells (ESCs), which in turn is essential for ESC self-renewal (By similarity).
Alternative names
PNAS-5, LYAR, Cell growth-regulating nucleolar protein
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Recombinant Human LYAR protein is a Human Full Length protein, in the 1 to 379 aa range, expressed in Wheat germ and suitable for ELISA, WB.
Key facts
- Expression system
- Wheat germ
- Tags
- GST tag N-Terminus
- Applications
- ELISA, WB
- Biologically active
- No
- Accession
- Q9NX58-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 8
Constituents: 0.79% Tris HCl, 0.31% Glutathione
Sequence info
Amino acid sequence
- M V F F T C N A C G E S V K K I Q V E K H V S V C R N C E C L S C I D C G K D F W G D D Y K N H V K C I S E D Q K Y G G K G Y E G K T H K G D I K Q Q A W I Q K I S E L I K R P N V S P K V R E L L E Q I S A F D N V P R K K A K F Q N W M K N S L K V H N E S I L D Q V W N I F S E A S N S E P V N K E Q D Q R P L H P V A N P H A E I S T K V P A S K V K D A V E Q Q G E V K K N K R E R K E E R Q K K R K R E K K E L K L E N H Q E N S R N Q K P K K R K K G Q E A D L E A G G E E V P E A N G S A G K R S K K K K Q R K D S A S E E E A R V G A G K R K R R H S E V E T D S K K K K M K L P E H P E G G E P E D D E A P A K G K F N W K G T I K A I L K Q A P D N E I T I K K L R K K V L A Q Y Y T V T D E H H R S E E E L L V I F N K K I S K N P T F K L L K D K V K L V K
- Accession
- Q9NX58
- Protein length
- Full Length
- Amino acids
- 1 to 379
- Nature
- Recombinant
- Tags
- GST tag N-Terminus
Specifications
- Form
- Liquid
General info
- Function
Plays a role in the maintenance of the appropriate processing of 47S/45S pre-rRNA to 32S/30S pre-rRNAs and their subsequent processing to produce 18S and 28S rRNAs (PubMed:24495227). Also acts at the level of transcription regulation. Along with PRMT5, binds the gamma-globin (HBG1/HBG2) promoter and represses its expression (PubMed:25092918). In neuroblastoma cells, may also repress the expression of oxidative stress genes, including CHAC1, HMOX1, SLC7A11, ULBP1 and SNORD41 that encodes a small nucleolar RNA (PubMed:28686580). Preferentially binds to a DNA motif containing 5'-GGTTAT-3' (PubMed:25092918). Negatively regulates the antiviral innate immune response by targeting IRF3 and impairing its DNA-binding activity (PubMed:31413131). In addition, inhibits NF-kappa-B-mediated expression of pro-inflammatory cytokines (PubMed:31413131). Stimulates phagocytosis of photoreceptor outer segments by retinal pigment epithelial cells (By similarity). Prevents nucleolin/NCL self-cleavage, maintaining a normal steady-state level of NCL protein in undifferentiated embryonic stem cells (ESCs), which in turn is essential for ESC self-renewal (By similarity).
- Subcellular localisation
- Nucleus, Nucleolus
Storage
- Shipped at conditions
- Dry Ice
- Appropriate short-term storage conditions
- -80°C
- Appropriate long-term storage conditions
- -80°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Lyar also known as the cell growth-regulating nucleolar protein is a zinc finger protein with a molecular mass of approximately 36 kDa. This protein is expressed primarily in the nucleolus although it can also be found in other sub-nuclear structures. Lyar binds to nucleic acids and proteins playing roles in nucleolus-related processes. Its expression is seen in various tissues but it is most pronounced in rapidly dividing cells such as those in fetal tissues and certain cancers.
- Biological function summary
The Lyar protein plays a role in regulating cell growth and proliferation. It participates in ribosome biogenesis by interacting with pre-ribosomal RNA possibly aiding in the stabilization and processing of rRNA precursors. Lyar is not usually considered as part of a protein complex but its interactions with several other nucleolar proteins imply its involvement in complex regulatory networks. It influences the transcription of ribosomal RNA genes impacting protein synthesis rates and cell cycle progression.
- Pathways
Lyar is involved in the modulation of pathways linked to cell proliferation and survival. Notably it affects the PI3K/AKT signaling pathway by interacting with nucleophosmin (NPM1) impacting cell growth and survival mechanisms. This collaboration can alter the response of cells to external growth signals influencing cellular responses to metabolic changes and stress.
- Associated diseases and disorders
Aberrant Lyar levels have been implicated in oncogenesis. Its overexpression has been noted in various cancers including leukemia and neuroblastoma linking it to uncontrolled cell division and tumor progression. In cancer Lyar interacts with tumorigenesis-associated proteins such as c-Myc enhancing their transcriptional activity and contributing to cancerous transformation. Understanding the role of Lyar in these disease processes remains essential for identifying potential therapeutic strategies and developing targeted interventions.
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1 product image
SDS-PAGE - Recombinant Human LYAR protein (ab163160)
ab163160 on a 12.5% SDS-PAGE stained with Coomassie Blue.
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