Recombinant human M-CSF protein (Active) (ab62015)

Recombinant human M-CSF protein (Active) is a Human Fragment protein, in the 33 to 190 aa range, expressed in Escherichia coli, with >95% purity, <= 1 EU/µg endotoxin level and suitable for FuncS, SDS-PAGE.

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Alternative names=Macrophage colony-stimulating factor 1, CSF-1, M-CSF, MCSF, Lanimostim, CSF1

Purity: >95% SDS-PAGE
Endotoxin level: <= 1 EU/µg
Expression system: Escherichia coli
Tags: Tag free
Applications: FuncS, SDS-PAGE
Biologically active: Yes
Sequence: M E E V S E Y C S H M I G S G H L Q S L Q R L I D S Q M E T S C Q I T F E F V D Q E Q L K D P V C Y L K K A F L L V Q D I M E D T M R F R D N T P N A I A I V Q L Q E L S L R L K S C F T K D Y E E H D K A C V R T F Y E T P L Q L L E K V K N V F N E T K N L L D K D W N I F S K N C N N S F A E C S S Q G H E R Q S E G S M E E V S E Y C S H M I G S G H L Q S L Q R L I D S Q M E T S C Q I T F E F V D Q E Q L K D P V C Y L K K A F L L V Q D I M E D T M R F R D N T P N A I A I V Q L Q E L S L R L K S C F T K D Y E E H D K A C V R T F Y E T P L Q L L E K V K N V F N E T K N L L D K D W N I F S K N C N N S F A E C S S Q G H E R Q S E G S

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Reactivity data

Application	Reactivity	Dilution info	Notes
Application FuncS	Reactivity Reacts	Dilution info -	Notes -
Application SDS-PAGE	Reactivity Reacts	Dilution info -	Notes Dimer: 37.1 kDa

Images

Target data

CSF1

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Alternative names=Macrophage colony-stimulating factor 1, CSF-1, M-CSF, MCSF, Lanimostim, CSF1

Key facts

Purity

>95% SDS-PAGE

Endotoxin level

<= 1 EU/µg

Expression system

Escherichia coli

Tags

Tag free

Applications

FuncS, SDS-PAGE

Biologically active

Yes

Biological activity

Method of Determination: NFS-60 cell proliferation

Acceptance Criteria: ED₅₀ ≤10 ng/mL(≥ 1.0 x 10^5 units/mg)

The specific activity of Human M-CSF is 1.0 x 10^5 IU/μg, which is calibrated against recombinant human M-CSF WHO International Standard.

Predicted molecular weight

37.1 kDa

Amino acids

33 to 190

Sequence

M E E V S E Y C S H M I G S G H L Q S L Q R L I D S Q M E T S C Q I T F E F V D Q E Q L K D P V C Y L K K A F L L V Q D I M E D T M R F R D N T P N A I A I V Q L Q E L S L R L K S C F T K D Y E E H D K A C V R T F Y E T P L Q L L E K V K N V F N E T K N L L D K D W N I F S K N C N N S F A E C S S Q G H E R Q S E G S M E E V S E Y C S H M I G S G H L Q S L Q R L I D S Q M E T S C Q I T F E F V D Q E Q L K D P V C Y L K K A F L L V Q D I M E D T M R F R D N T P N A I A I V Q L Q E L S L R L K S C F T K D Y E E H D K A C V R T F Y E T P L Q L L E K V K N V F N E T K N L L D K D W N I F S K N C N N S F A E C S S Q G H E R Q S E G S

Accession

P09603-1

Protein length

Fragment

Animal free

Carrier free

Yes

Nature

Recombinant

Species

Human

Reconstitution

Sterile water at 0.1 mg/mL Centrifuge vial before opening. Suspend the product by gently pipetting the above recommended solution down the sides of the vial. DO NOT VORTEX. Allow several minutes for complete reconstitution.

Concentration

Storage buffer

Constituents: 0.58% Sodium chloride, 0.16% Sodium phosphate

Specifications

Form: Lyophilized

General info

Function: Cytokine that plays an essential role in the regulation of survival, proliferation and differentiation of hematopoietic precursor cells, especially mononuclear phagocytes, such as macrophages and monocytes. Promotes the release of proinflammatory chemokines, and thereby plays an important role in innate immunity and in inflammatory processes. Plays an important role in the regulation of osteoclast proliferation and differentiation, the regulation of bone resorption, and is required for normal bone development. Required for normal male and female fertility. Promotes reorganization of the actin cytoskeleton, regulates formation of membrane ruffles, cell adhesion and cell migration. Plays a role in lipoprotein clearance.
Post-translational modifications: N- and O-glycosylated. Glycosylation and proteolytic cleavage yield different soluble forms. One high molecular weight soluble form is a proteoglycan containing chondroitin sulfate. O-glycosylated with core 1 or possibly core 8 glycans. Isoform 1 is N- and O-glycosylated. Isoform 3 is only N-glycosylated.

Storage

Shipped at conditions: Ambient - Can Ship with Ice
Appropriate long-term storage conditions: -80°C
Aliquoting information: Upon reconstitution add a carrier protein (0.1% BSA)
Storage information: Avoid freeze / thaw cycle

This product is an active protein and may elicit a biological response in vivo, handle with caution.

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Recombinant human M-CSF protein (Active)

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Reactivity data

Images

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