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AB114358

Recombinant Human MADH7/SMAD7 protein (GST tag N-Terminus)

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Recombinant Human MADH7/SMAD7 protein (GST tag N-Terminus) is a Human Fragment protein, in the 160 to 260 aa range, expressed in Wheat germ, suitable for SDS-PAGE, ELISA, WB.

View Alternative Names

MADH7, MADH8, SMAD7, SMAD family member 7, SMAD 7, hSMAD7, Mothers against decapentaplegic homolog 7, Mothers against decapentaplegic homolog 8, MAD homolog 7, Mothers against DPP homolog 7, MAD homolog 8, Mothers against DPP homolog 8

1 Images
SDS-PAGE - Recombinant Human MADH7/SMAD7 protein (GST tag N-Terminus) (AB114358)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant Human MADH7/SMAD7 protein (GST tag N-Terminus) (AB114358)

12.5% SDS-PAGE showing ab114358 at approximately 36.74kDa stained with Coomassie Blue.

Key facts

Expression system

Wheat germ

Tags

GST tag N-Terminus

Applications

WB, SDS-PAGE, ELISA

applications

Biologically active

No

Accession

O15105

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 8 Constituents: 0.79% Tris HCl, 0.3% Glutathione

storage-buffer

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Complementary Products

Primary Antibodies

AB216428

Anti-MADH7/SMAD7 antibody

Western blot - Anti-MADH7/SMAD7 antibody (AB216428)

  • 4
  • 1
Primary Antibodies

AB202445

Anti-Smad2 + Smad3 antibody [EPR19557-4] - ChIP Grade

20ul selling size|RabMAb|Advanced Validation|Recombinant

Flow Cytometry (Intracellular) - Anti-Smad2 + Smad3 antibody [EPR19557-4] - ChIP Grade (AB202445)

  • 3
  • 1
Primary Antibodies

AB259360

Anti-TGF beta Receptor II antibody [EPR24349-124]

20ul selling size|KO Validated|RabMAb|Recombinant

Immunoprecipitation - Anti-TGF beta Receptor II antibody [EPR24349-124] (AB259360)

  • 0
  • 0
Proteins & Peptides

AB81764

Recombinant Human Smad4 protein (His tag N-Terminus)

Western blot - Recombinant Human Smad4 protein (His tag N-Terminus) (AB81764)

  • 0
  • 0
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "ELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p>(Recombinant protein).</p>" } } }
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Sequence info

[{"sequence":"CKVFRWPDLRHSSEVKRLCCCESYGKINPELVCCNPHHLSRLCELESPPPPYSRYPMDFLKPTADCPDAVPSSAETGGTNYLAPGGLSDSQLLLEPGDRSH","proteinLength":"Fragment","predictedMolecularWeight":"36.74 kDa","actualMolecularWeight":null,"aminoAcidEnd":260,"aminoAcidStart":160,"nature":"Recombinant","expressionSystem":"Wheat germ","accessionNumber":"O15105","tags":[{"tag":"GST","terminus":"N-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Dry Ice
Appropriate short-term storage conditions
-80°C
Appropriate long-term storage conditions
-80°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

MADH7 also known as SMAD7 is an intracellular protein that acts as an inhibitor in the signaling pathway of the TGF-beta (transforming growth factor-beta) family. This protein has a molecular mass of approximately 46 kDa and is widely expressed with a significant presence in tissues involved in immune response and development. SMAD7 directly interacts with receptor-regulated SMADs (R-SMADs) preventing their phosphorylation and consequent nuclear translocation effectively halting further signal transduction.
Biological function summary

SMAD7 functions to regulate the TGF-beta signaling pathway by operating as a negative feedback mediator. It binds to TGF-beta receptors recruiting E3 ubiquitin ligases which promote receptor degradation. SMAD7 is not part of complex large-scale multi-protein assemblies but is essential in modulating the pathway's activity. Its balance controls important processes like cell proliferation differentiation and apoptosis across varied biological contexts.

Pathways

SMAD7 strongly influences the TGF-beta and BMP (bone morphogenetic protein) signaling pathways. SMAD7 blocks TGF-beta signaling by interfering with the activity of R-SMADs such as SMAD2 and SMAD3 and it modulates the BMP pathway by interacting with SMAD1 and SMAD5. Its regulatory roles are tightly integrated within these pathways highlighting its importance in cellular homeostasis and response to extracellular signals.

SMAD7 has links to inflammatory conditions and cancer. Its dysregulation can lead to heightened TGF-beta signaling which may contribute to conditions like fibrosis and can enhance tumor progression by affecting cancer cell dynamics and the tumor microenvironment. In these scenarios abnormal SMAD7 function can associate with proteins such as SMAD3 in fibrosis while in cancer its association with proteins like SMAD4 disrupts normal growth control and cellular response.
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Specifications

Form

Liquid

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General info

Function

Antagonist of signaling by TGF-beta (transforming growth factor) type 1 receptor superfamily members; has been shown to inhibit TGF-beta (Transforming growth factor) and activin signaling by associating with their receptors thus preventing SMAD2 access (PubMed : 21791611). Functions as an adapter to recruit SMURF2 to the TGF-beta receptor complex. Also acts by recruiting the PPP1R15A-PP1 complex to TGFBR1, which promotes its dephosphorylation. Positively regulates PDPK1 kinase activity by stimulating its dissociation from the 14-3-3 protein YWHAQ which acts as a negative regulator.

Sequence similarities

Belongs to the dwarfin/SMAD family.

Post-translational modifications

Phosphorylation on Ser-249 does not affect its stability, nuclear localization or inhibitory function in TGFB signaling; however it affects its ability to regulate transcription (By similarity). Phosphorylated by PDPK1.. Ubiquitinated by WWP1 (By similarity). Polyubiquitinated by RNF111, which is enhanced by AXIN1 and promotes proteasomal degradation (PubMed:14657019, PubMed:16601693). In response to TGF-beta, ubiquitinated by SMURF1; which promotes its degradation (PubMed:11278251).. Acetylation prevents ubiquitination and degradation mediated by SMURF1.

Subcellular localisation

Nucleus

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Product protocols

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Target data

Antagonist of signaling by TGF-beta (transforming growth factor) type 1 receptor superfamily members; has been shown to inhibit TGF-beta (Transforming growth factor) and activin signaling by associating with their receptors thus preventing SMAD2 access (PubMed : 21791611). Functions as an adapter to recruit SMURF2 to the TGF-beta receptor complex. Also acts by recruiting the PPP1R15A-PP1 complex to TGFBR1, which promotes its dephosphorylation. Positively regulates PDPK1 kinase activity by stimulating its dissociation from the 14-3-3 protein YWHAQ which acts as a negative regulator.
See full target information SMAD7
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Product promise

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