Recombinant Human MADM protein is a Human Full Length protein, in the 1 to 535 aa range, expressed in Baculovirus infected Sf9, with >90% purity and suitable for SDS-PAGE.
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Key facts
- Purity
- >90% SDS-PAGE
- Expression system
- Baculovirus infected Sf9 cells
- Tags
- GST tag N-Terminus
- Applications
- SDS-PAGE
- Biologically active
- No
Amino acid sequence
M S E G E S Q T V L S S G S D P K V E S S S S A P G L T S V S P P V T S T T S A A S P E E E E E S E D E S E I L E E S P C G R W Q K R R E E V N Q R N V P G I D S A Y L A M D T E E G V E V V W N E V Q F S E R K N Y K L Q E E K V R A V F D N L I Q L E H L N I V K F H K Y W A D I K E N K A R V I F I T E Y M S S G S L K Q F L K K T K K N H K T M N E K A W K R W C T Q I L S A L S Y L H S C D P P I I H G N L T C D T I F I Q H N G L I K I G S V A P D T I N N H V K T C R E E Q K N L H F F A P E Y G E V T N V T T A V D I Y S F G M C A L E M A V L E I Q G N G E S S Y V P Q E A I S S A I Q L L E D P L Q R E F I Q K C L Q S E P A R R P T A R E L L F H P A L F E V P S L K L L A A H C I V G H Q H M I P E N A L E E I T K N M D T S A V L A E I P A G P G R E P V Q T L Y S Q S P A L E L D K F L E D V R N G I Y P L T A F G L P R P Q Q P Q Q E E V T S P V V P P S V K T P T P E P A E V E T R K V V L M Q C N I E S V E E G V K H H L T L L L K L E D K L N R H L S C D L M P N E N I P E L A A E L V Q L G F I S E A D Q S R L T S L L E E T L N K F N F A R N S T L N S A A V T V S S
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Required for embryonic development (By similarity). Plays a role in intestinal epithelial cell fate and proliferation, thereby involved in the architectural development of the intestine potentially via the regulation of Wnt-responsive genes (By similarity). May play a role in subcellular trafficking between the endoplasmic reticulum and Golgi apparatus through interactions with the Rho-type GTPases (PubMed:11956649). Binding to the NS3 protein of dengue virus type 2 appears to subvert this activity into the alteration of the intracellular membrane structure associated with flaviviral replication (PubMed:15084397).
Alternative names
BCON3, NRBP, NRBP1, Nuclear receptor-binding protein
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Recombinant Human MADM protein is a Human Full Length protein, in the 1 to 535 aa range, expressed in Baculovirus infected Sf9, with >90% purity and suitable for SDS-PAGE.
Key facts
- Purity
- >90% SDS-PAGE
- Expression system
- Baculovirus infected Sf9 cells
- Tags
- GST tag N-Terminus
- Applications
- SDS-PAGE
- Biologically active
- No
- Accession
- Q9UHY1-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 7.5
Constituents: 25% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, 0.79% Tris HCl, 0.31% Glutathione, 0.004% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.003% EDTA, 0.002% PMSF
Sequence info
Amino acid sequence
- M S E G E S Q T V L S S G S D P K V E S S S S A P G L T S V S P P V T S T T S A A S P E E E E E S E D E S E I L E E S P C G R W Q K R R E E V N Q R N V P G I D S A Y L A M D T E E G V E V V W N E V Q F S E R K N Y K L Q E E K V R A V F D N L I Q L E H L N I V K F H K Y W A D I K E N K A R V I F I T E Y M S S G S L K Q F L K K T K K N H K T M N E K A W K R W C T Q I L S A L S Y L H S C D P P I I H G N L T C D T I F I Q H N G L I K I G S V A P D T I N N H V K T C R E E Q K N L H F F A P E Y G E V T N V T T A V D I Y S F G M C A L E M A V L E I Q G N G E S S Y V P Q E A I S S A I Q L L E D P L Q R E F I Q K C L Q S E P A R R P T A R E L L F H P A L F E V P S L K L L A A H C I V G H Q H M I P E N A L E E I T K N M D T S A V L A E I P A G P G R E P V Q T L Y S Q S P A L E L D K F L E D V R N G I Y P L T A F G L P R P Q Q P Q Q E E V T S P V V P P S V K T P T P E P A E V E T R K V V L M Q C N I E S V E E G V K H H L T L L L K L E D K L N R H L S C D L M P N E N I P E L A A E L V Q L G F I S E A D Q S R L T S L L E E T L N K F N F A R N S T L N S A A V T V S S
- Accession
- Q9UHY1
- Protein length
- Full Length
- Predicted molecular weight
- 92 kDa
- Amino acids
- 1 to 535
- Nature
- Recombinant
- Tags
- GST tag N-Terminus
Specifications
- Form
- Liquid
- Additional notes
Assessed by densitometry. Affinity purified.
General info
- Function
Required for embryonic development (By similarity). Plays a role in intestinal epithelial cell fate and proliferation, thereby involved in the architectural development of the intestine potentially via the regulation of Wnt-responsive genes (By similarity). May play a role in subcellular trafficking between the endoplasmic reticulum and Golgi apparatus through interactions with the Rho-type GTPases (PubMed:11956649). Binding to the NS3 protein of dengue virus type 2 appears to subvert this activity into the alteration of the intracellular membrane structure associated with flaviviral replication (PubMed:15084397).
- Sequence similarities
Belongs to the protein kinase superfamily. Ser/Thr protein kinase family.
Storage
- Shipped at conditions
- Dry Ice
- Appropriate short-term storage conditions
- -80°C
- Appropriate long-term storage conditions
- -80°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The Misexpression Affecting Delta Microtubule (MADM) protein also known as 'mαδμ' is involved in the regulation of microtubule dynamics important for cellular structure and transport. This protein although its precise molecular weight is not well-documented generally localizes within cells that actively undergo division and differentiation across various tissues. The expression of MADM can vary significantly often reflecting the differentiation status of the cell and tissue involved.
- Biological function summary
MADM interacts with several cellular proteins helping to orchestrate the assembly and disassembly of microtubules. It does not function in isolation and often forms complexes with other proteins to effectively manage its roles. Through these interactions MADM ensures proper cell division and migration key processes in maintaining cellular integrity and tissue development.
- Pathways
MADM integrates into the signaling networks governing the cytoskeleton. It participates in the microtubule-organizing center (MTOC) pathways and is associated with the mitotic spindle organization. During these processes MADM interacts with proteins like α-tubulin and β-tubulin facilitating spindle functionality essential for accurate chromosome segregation during mitosis.
- Associated diseases and disorders
Abnormal expression or mutation in MADM has correlations with certain cancers such as breast and ovarian cancer. This association likely involves its link with tumor suppressor proteins like p53 and BRCA1. Dysregulation in MADM affects the maintenance of genomic stability contributing to oncogenesis and highlighting its therapeutic potential as a target in cancer treatment strategies.
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SDS-PAGE - Recombinant Human MADM protein (ab204143)
SDS-PAGE analysis of ab204143.
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