Recombinant Human MAF1 protein
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Recombinant Human MAF1 protein is a Human Full Length protein, in the 1 to 256 aa range, expressed in Escherichia coli, with >90%, suitable for SDS-PAGE, Mass Spec.
View Alternative Names
Repressor of RNA polymerase III transcription MAF1 homolog, MAF1
- SDS-PAGE
Unknown
SDS-PAGE - Recombinant Human MAF1 protein (AB111643)
15% SDS-PAGE analysis of ab111643 (3 μg).
Reactivity data
Sequence info
Properties and storage information
Shipped at conditions
Appropriate short-term storage duration
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Aliquoting information
Storage information
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
MAF1 is significant in cellular growth and metabolism regulation. It is not part of a larger protein complex but interacts with several proteins to exert its regulatory functions. MAF1 acts as a brake on RNA polymerase III activity responding to changes in cellular environmental conditions such as nutrient availability and stress. By doing so MAF1 helps coordinate cellular responses to shifts in energy levels and stress modulating the balance between cell growth and maintenance.
Pathways
MAF1 plays a role in the mTOR signaling pathway which is important for cell growth proliferation and survival. Within this pathway MAF1 functions as a downstream effector that inhibits RNA transcription when mTORC1 activity decreases. It relates closely to other proteins such as mTOR and AKT which are also involved in cellular responses to nutrient and energy fluctuations. By influencing RNA polymerase III transcription MAF1 contributes to the regulation of protein synthesis according to metabolic needs.
Specifications
Form
Liquid
Additional notes
ab111643 is purified using conventional chromatography.
General info
Function
Plays a role in the repression of RNA polymerase III-mediated transcription in response to changing nutritional, environmental and cellular stress conditions to balance the production of highly abundant tRNAs, 5S rRNA, and other small non-coding RNAs with cell growth and maintenance (PubMed : 18377933, PubMed : 20233713, PubMed : 20516213, PubMed : 20543138). Also plays a key role in cell fate determination by promoting mesorderm induction and adipocyte differentiation (By similarity). Mechanistically, associates with the RNA polymerase III clamp and thereby impairs its recruitment to the complex made of the promoter DNA, TBP and the initiation factor TFIIIB (PubMed : 17505538, PubMed : 20887893). When nutrients are available and mTOR kinase is active, MAF1 is hyperphosphorylated and RNA polymerase III is engaged in transcription. Stress-induced MAF1 dephosphorylation results in nuclear localization, increased targeting of gene-bound RNA polymerase III and a decrease in the transcriptional readout (PubMed : 26941251). Additionally, may also regulate RNA polymerase I and RNA polymerase II-dependent transcription through its ability to regulate expression of the central initiation factor TBP (PubMed : 17499043).
Sequence similarities
Belongs to the MAF1 family.
Post-translational modifications
Phosphorylated at Ser-60, Ser-68 and Ser-75; the major sites of phosphorylation. Nuclear accumulation correlates with a concomitant dephosphorylation. Phosphorylation may attenuate its RNA polymerase III-repressive function.. Sumoylated with SUMO1 and SUMO2, mainly on Lys-35. Desumoylated by SENP1. SUMOylation promotes the ability of MAF1 to repress transcription and suppress colony formation.
Subcellular localisation
Nucleus
Target data
Complementary Products
Product promise
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