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AB113589

Recombinant Human MAFG protein (His tag N-Terminus)

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Recombinant Human MAFG protein (His tag N-Terminus) is a Human Full Length protein, in the 1 to 162 aa range, expressed in Escherichia coli, with >90%, suitable for SDS-PAGE, Mass Spec.

View Alternative Names

Transcription factor MafG, V-maf musculoaponeurotic fibrosarcoma oncogene homolog G, hMAF, MAFG

1 Images
SDS-PAGE - Recombinant Human MAFG protein (His tag N-Terminus) (AB113589)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant Human MAFG protein (His tag N-Terminus) (AB113589)

3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain.

false

Key facts

Purity

>90% SDS-PAGE

Expression system

Escherichia coli

Tags

His tag N-Terminus

Applications

SDS-PAGE, Mass Spec

applications

Biologically active

No

Accession

O15525

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 8 Constituents: 40% Glycerol (glycerin, glycerine), 0.88% Sodium chloride, 0.32% Tris HCl, 0.02% (R*,R*)-1,4-Dimercaptobutan-2,3-diol

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "Mass Spec": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Sequence info

[{"sequence":"","proteinLength":"Full Length","predictedMolecularWeight":"20 kDa","actualMolecularWeight":null,"aminoAcidEnd":162,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":null,"accessionNumber":"O15525","tags":[{"tag":"His","terminus":"N-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

MAFG also known as MAFG transcription factor is a small basic leucine zipper (bZIP) protein that weighs approximately 18 kDa. It belongs to the small MAF protein family and has a role in gene regulation. MAFG is widely expressed in various tissues but shows higher expression in the liver and lungs. Functionally MAFG acts as a transcriptional repressor by binding to Maf recognition elements (MAREs) in the DNA regulating genes involved in cellular stress responses and homeostasis.
Biological function summary

The MAFG transcription factor forms complexes with other small MAF proteins like MAFF and MAFK or larger bZIP proteins. These complexes modulate transcription of antioxidant response genes such as those encoding phase II detoxifying enzymes. MAFG is important for the regulation of oxidative stress genes. The interaction of MAFG with other proteins determines whether it functions as a repressor or an activator influencing cellular responses to various stimuli.

Pathways

MAFG plays an important role in the oxidative stress response and the Nrf2 signaling pathway. It interacts closely with Nrf2 an important transcription factor activating the expression of antioxidant proteins to protect against oxidative damage. MAFG and Nrf2 along with other proteins facilitate cellular defense mechanisms by maintaining redox balance and regulating detoxification processes.

MAFG links to conditions such as cancer and neurodegenerative diseases. Aberrant expression of MAFG may contribute to tumor development and progression by disrupting cellular homeostasis and detoxification pathways. Additionally MAFG dysregulation can affect the Nrf2 pathway impacting neurodegenerative disorders like Alzheimer's disease. Therefore understanding MAFG's interaction with Nrf2 and other proteins can provide insights into therapeutic strategies targeting these diseases.
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Specifications

Form

Liquid

Additional notes

ab113589 was purified by using conventional chromatography.

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General info

Function

Since they lack a putative transactivation domain, the small Mafs behave as transcriptional repressors when they dimerize among themselves (PubMed : 11154691). However, they seem to serve as transcriptional activators by dimerizing with other (usually larger) basic-zipper proteins, such as NFE2, NFE2L1 and NFE2L2, and recruiting them to specific DNA-binding sites (PubMed : 11154691, PubMed : 8932385, PubMed : 9421508). Small Maf proteins heterodimerize with Fos and may act as competitive repressors of the NFE2L2 transcription factor (PubMed : 11154691). Transcription factor, component of erythroid-specific transcription factor NFE2L2 (PubMed : 11154691). Activates globin gene expression when associated with NFE2L2 (PubMed : 11154691). May be involved in signal transduction of extracellular H(+) (By similarity).

Sequence similarities

Belongs to the bZIP family. Maf subfamily.

Post-translational modifications

Acetylated in erythroid cells by CREB-binding protein (CBP). Acetylation augments the DNA-binding activity of NFE2, but has no effect on binding NFE2.. Sumoylation at Lys-14 is required for active transcriptional repression.

Subcellular localisation

Nucleus

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Product protocols

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Target data

Since they lack a putative transactivation domain, the small Mafs behave as transcriptional repressors when they dimerize among themselves (PubMed : 11154691). However, they seem to serve as transcriptional activators by dimerizing with other (usually larger) basic-zipper proteins, such as NFE2, NFE2L1 and NFE2L2, and recruiting them to specific DNA-binding sites (PubMed : 11154691, PubMed : 8932385, PubMed : 9421508). Small Maf proteins heterodimerize with Fos and may act as competitive repressors of the NFE2L2 transcription factor (PubMed : 11154691). Transcription factor, component of erythroid-specific transcription factor NFE2L2 (PubMed : 11154691). Activates globin gene expression when associated with NFE2L2 (PubMed : 11154691). May be involved in signal transduction of extracellular H(+) (By similarity).
See full target information MAFG
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