Recombinant Human MAFK protein (denatured) is a Human Full Length protein, in the 1 to 156 aa range, expressed in Escherichia coli, with >85% purity and suitable for SDS-PAGE.
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Key facts
- Purity
- >85% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE
- Biologically active
- No
Amino acid sequence
M G S S H H H H H H S S G L V P R G S H M T T N P K P N K A L K V K K E A G E N A P V L S D D E L V S M S V R E L N Q H L R G L T K E E V T R L K Q R R R T L K N R G Y A A S C R I K R V T Q K E E L E R Q R V E L Q Q E V E K L A R E N S S M R L E L D A L R S K Y E A L Q T F A R T V A R G P V A P S K V A T T S V I T I V K S T E L S S T S V P F S A A S
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Since they lack a putative transactivation domain, the small Mafs behave as transcriptional repressors when they dimerize among themselves (PubMed:9150357). However, they act as transcriptional activators by dimerizing with other (usually larger) basic-zipper proteins, such as NFE2, NFE2L1/NRF1, NFE2L2/NRF2 and NFE2L3/NRF3, and recruiting them to specific DNA-binding sites (PubMed:8932385, PubMed:9150357). Small Maf proteins heterodimerize with Fos and may act as competitive repressors of the NF-E2 transcription factor (PubMed:9150357).
Alternative names
Transcription factor MafK, Erythroid transcription factor NF-E2 p18 subunit, MAFK
Recommended products
Recombinant Human MAFK protein (denatured) is a Human Full Length protein, in the 1 to 156 aa range, expressed in Escherichia coli, with >85% purity and suitable for SDS-PAGE.
Key facts
- Purity
- >85% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE
- Biologically active
- No
- Accession
- O60675-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 8
Constituents: 10% Glycerol (glycerin, glycerine), 2.4% Urea, 0.32% Tris HCl
Sequence info
Amino acid sequence
- M G S S H H H H H H S S G L V P R G S H M T T N P K P N K A L K V K K E A G E N A P V L S D D E L V S M S V R E L N Q H L R G L T K E E V T R L K Q R R R T L K N R G Y A A S C R I K R V T Q K E E L E R Q R V E L Q Q E V E K L A R E N S S M R L E L D A L R S K Y E A L Q T F A R T V A R G P V A P S K V A T T S V I T I V K S T E L S S T S V P F S A A S
- Accession
- O60675
- Protein length
- Full Length
- Predicted molecular weight
- 19.7 kDa
- Amino acids
- 1 to 156
- Nature
- Recombinant
- Tags
- His tag N-Terminus
Specifications
- Form
- Liquid
General info
- Function
Since they lack a putative transactivation domain, the small Mafs behave as transcriptional repressors when they dimerize among themselves (PubMed:9150357). However, they act as transcriptional activators by dimerizing with other (usually larger) basic-zipper proteins, such as NFE2, NFE2L1/NRF1, NFE2L2/NRF2 and NFE2L3/NRF3, and recruiting them to specific DNA-binding sites (PubMed:8932385, PubMed:9150357). Small Maf proteins heterodimerize with Fos and may act as competitive repressors of the NF-E2 transcription factor (PubMed:9150357).
- Sequence similarities
Belongs to the bZIP family. Maf subfamily.
- Subcellular localisation
- Nucleus
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
MAFK also known as Small Maf protein functions as a transcription factor involved in gene regulation. It has a molecular weight of approximately 18 kDa. This protein mainly acts as a repressor that binds to Maf recognition elements (MAREs) in DNA. Expression of MAFK occurs in various tissues including liver brain and lung. Its ability to form dimers further influences its regulatory activity enabling it to participate in diverse cellular processes.
- Biological function summary
MAFK regulates transcription by forming heterodimers with other proteins such as the CNC-bZIP family members including NFE2L2 (also called NRF2). In these complexes MAFK modulates antioxidant response elements (ARE) gene expression. By controlling genes involved in oxidative stress responses MAFK plays an important role in maintaining cellular homeostasis. This functionality allows MAFK to impact a broad range of cellular activities reinforcing its importance in cellular responses to stress.
- Pathways
MAFK is heavily involved in the oxidative stress response and the xenobiotic metabolism pathway. Through its interaction with NRF2 MAFK influences the cellular defense mechanism against oxidative damage highlighting its role in the NRF2-ARE pathway. Moreover MAFK also intersects with the heme degradation pathway where it collaborates with heme oxygenase 1 (HO-1) regulation. By participating in these pathways MAFK supports cellular adaptation to environmental changes.
- Associated diseases and disorders
MAFK has connections to cancer and neurodegenerative diseases. In various cancers altered MAFK expression impacts tumor growth and progression partly through NRF2-related pathways. Abnormal regulation of MAFK can also relate to disorders like Parkinson's disease where oxidative stress mechanisms are dysregulated. In these contexts the protein interacts with others like KEAP1 a regulator of NRF2 activity to influence disease development and progression.
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1 product image
SDS-PAGE - Recombinant Human MAFK protein (denatured) (ab139794)
15% SDS-PAGE analysis of ab139794 (3μg).
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