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AB139794

Recombinant Human MAFK protein (denatured) (His tag N-Terminus)

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Recombinant Human MAFK protein (denatured) (His tag N-Terminus) is a Human Full Length protein, in the 1 to 156 aa range, expressed in Escherichia coli, with >85%, suitable for SDS-PAGE.

View Alternative Names

Transcription factor MafK, Erythroid transcription factor NF-E2 p18 subunit, MAFK

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SDS-PAGE - Recombinant Human MAFK protein (denatured) (His tag N-Terminus) (AB139794)
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Supplier Data

SDS-PAGE - Recombinant Human MAFK protein (denatured) (His tag N-Terminus) (AB139794)

15% SDS-PAGE analysis of ab139794 (3μg).

Key facts

Purity

>85% SDS-PAGE

Expression system

Escherichia coli

Tags

His tag N-Terminus

Applications

SDS-PAGE

applications

Biologically active

No

Accession

O60675

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 8 Constituents: 10% Glycerol (glycerin, glycerine), 2.4% Urea, 0.32% Tris HCl

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Sequence info

[{"sequence":"MGSSHHHHHHSSGLVPRGSHMTTNPKPNKALKVKKEAGENAPVLSDDELVSMSVRELNQHLRGLTKEEVTRLKQRRRTLKNRGYAASCRIKRVTQKEELERQRVELQQEVEKLARENSSMRLELDALRSKYEALQTFARTVARGPVAPSKVATTSVITIVKSTELSSTSVPFSAAS","proteinLength":"Full Length","predictedMolecularWeight":"19.7 kDa","actualMolecularWeight":null,"aminoAcidEnd":156,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"O60675","tags":[{"tag":"His","terminus":"N-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

MAFK also known as Small Maf protein functions as a transcription factor involved in gene regulation. It has a molecular weight of approximately 18 kDa. This protein mainly acts as a repressor that binds to Maf recognition elements (MAREs) in DNA. Expression of MAFK occurs in various tissues including liver brain and lung. Its ability to form dimers further influences its regulatory activity enabling it to participate in diverse cellular processes.
Biological function summary

MAFK regulates transcription by forming heterodimers with other proteins such as the CNC-bZIP family members including NFE2L2 (also called NRF2). In these complexes MAFK modulates antioxidant response elements (ARE) gene expression. By controlling genes involved in oxidative stress responses MAFK plays an important role in maintaining cellular homeostasis. This functionality allows MAFK to impact a broad range of cellular activities reinforcing its importance in cellular responses to stress.

Pathways

MAFK is heavily involved in the oxidative stress response and the xenobiotic metabolism pathway. Through its interaction with NRF2 MAFK influences the cellular defense mechanism against oxidative damage highlighting its role in the NRF2-ARE pathway. Moreover MAFK also intersects with the heme degradation pathway where it collaborates with heme oxygenase 1 (HO-1) regulation. By participating in these pathways MAFK supports cellular adaptation to environmental changes.

MAFK has connections to cancer and neurodegenerative diseases. In various cancers altered MAFK expression impacts tumor growth and progression partly through NRF2-related pathways. Abnormal regulation of MAFK can also relate to disorders like Parkinson's disease where oxidative stress mechanisms are dysregulated. In these contexts the protein interacts with others like KEAP1 a regulator of NRF2 activity to influence disease development and progression.
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Specifications

Form

Liquid

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General info

Function

Since they lack a putative transactivation domain, the small Mafs behave as transcriptional repressors when they dimerize among themselves (PubMed : 9150357). However, they act as transcriptional activators by dimerizing with other (usually larger) basic-zipper proteins, such as NFE2, NFE2L1/NRF1, NFE2L2/NRF2 and NFE2L3/NRF3, and recruiting them to specific DNA-binding sites (PubMed : 8932385, PubMed : 9150357). Small Maf proteins heterodimerize with Fos and may act as competitive repressors of the NF-E2 transcription factor (PubMed : 9150357).

Sequence similarities

Belongs to the bZIP family. Maf subfamily.

Subcellular localisation

Nucleus

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Product protocols

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Target data

Since they lack a putative transactivation domain, the small Mafs behave as transcriptional repressors when they dimerize among themselves (PubMed : 9150357). However, they act as transcriptional activators by dimerizing with other (usually larger) basic-zipper proteins, such as NFE2, NFE2L1/NRF1, NFE2L2/NRF2 and NFE2L3/NRF3, and recruiting them to specific DNA-binding sites (PubMed : 8932385, PubMed : 9150357). Small Maf proteins heterodimerize with Fos and may act as competitive repressors of the NF-E2 transcription factor (PubMed : 9150357).
See full target information MAFK
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