Recombinant Human MAG / GMA Protein is a Human Fragment protein, in the 20 to 516 aa range, expressed in HEK 293, with >=95% purity and suitable for SDS-PAGE.
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Key facts
- Purity
- >=95% HPLC
- Expression system
- HEK 293 cells
- Tags
- Tag free
- Applications
- SDS-PAGE
- Biologically active
- No
Amino acid sequence
H W G A W M P S S I S A F E G T C V S I P C R F D F P D E L R P A V V H G V W Y F N S P Y P K N Y P P V V F K S R T Q V V H E S F Q G R S R L L G D L G L R N C T L L L S N V S P E L G G K Y Y F R G D L G G Y N Q Y T F S E H S V L D I V N T P N I V V P P E V V A G T E V E V S C M V P D N C P E L R P E L S W L G H E G L G E P A V L G R L R E D E G T W V Q V S L L H F V P T R E A N G H R L G C Q A S F P N T T L Q F E G Y A S M D V K Y P P V I V E M N S S V E A I E G S H V S L L C G A D S N P P P L L T W M R D G T V L R E A V A E S L L L E L E E V T P A E D G V Y A C L A E N A Y G Q D N R T V G L S V M Y A P W K P T V N G T M V A V E G E T V S I L C S T Q S N P D P I L T I F K E K Q I L S T V I Y E S E L Q L E L P A V S P E D D G E Y W C V A E N Q Y G Q R A T A F N L S V E F A P V L L L E S H C A A A R D T V Q C L C V V K S N P E P S V A F E L P S R N V T V N E S E R E F V Y S E R S G L V L T S I L T L R G Q A Q A P P R V I C T A R N L Y G A K S L E L P F Q G A H R L M W A K I G P
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Adhesion molecule that mediates interactions between myelinating cells and neurons by binding to neuronal sialic acid-containing gangliosides and to the glycoproteins RTN4R and RTN4RL2 (By similarity). Not required for initial myelination, but seems to play a role in the maintenance of normal axon myelination. Protects motoneurons against apoptosis, also after injury; protection against apoptosis is probably mediated via interaction with neuronal RTN4R and RTN4RL2. Required to prevent degeneration of myelinated axons in adults; this probably depends on binding to gangliosides on the axon cell membrane (By similarity). Negative regulator of neurite outgrowth; in dorsal root ganglion neurons the inhibition is mediated primarily via binding to neuronal RTN4R or RTN4RL2 and to a lesser degree via binding to neuronal gangliosides. In cerebellar granule cells the inhibition is mediated primarily via binding to neuronal gangliosides. In sensory neurons, inhibition of neurite extension depends only partially on RTN4R, RTN4RL2 and gangliosides. Inhibits axon longitudinal growth (By similarity). Inhibits axon outgrowth by binding to RTN4R (By similarity). Preferentially binds to alpha-2,3-linked sialic acid. Binds ganglioside Gt1b (By similarity).
Alternative names
GMA, MAG, Myelin-associated glycoprotein, Siglec-4a
Recommended products
Recombinant Human MAG / GMA Protein is a Human Fragment protein, in the 20 to 516 aa range, expressed in HEK 293, with >=95% purity and suitable for SDS-PAGE.
Key facts
- Purity
- >=95% HPLC
- Expression system
- HEK 293 cells
- Tags
- Tag free
- Applications
- SDS-PAGE
- Biologically active
- No
- Accession
- P20916-1
- Animal free
- Yes
- Species
- Human
- Reconstitution
- Reconstitute in PBS
- Concentration
- Storage buffer
pH: 7.4
Constituents: 10.26% Trehalose, 0.727% Dibasic monohydrogen potassium phosphate, 0.248% Potassium phosphate monobasic
Sequence info
Amino acid sequence
- H W G A W M P S S I S A F E G T C V S I P C R F D F P D E L R P A V V H G V W Y F N S P Y P K N Y P P V V F K S R T Q V V H E S F Q G R S R L L G D L G L R N C T L L L S N V S P E L G G K Y Y F R G D L G G Y N Q Y T F S E H S V L D I V N T P N I V V P P E V V A G T E V E V S C M V P D N C P E L R P E L S W L G H E G L G E P A V L G R L R E D E G T W V Q V S L L H F V P T R E A N G H R L G C Q A S F P N T T L Q F E G Y A S M D V K Y P P V I V E M N S S V E A I E G S H V S L L C G A D S N P P P L L T W M R D G T V L R E A V A E S L L L E L E E V T P A E D G V Y A C L A E N A Y G Q D N R T V G L S V M Y A P W K P T V N G T M V A V E G E T V S I L C S T Q S N P D P I L T I F K E K Q I L S T V I Y E S E L Q L E L P A V S P E D D G E Y W C V A E N Q Y G Q R A T A F N L S V E F A P V L L L E S H C A A A R D T V Q C L C V V K S N P E P S V A F E L P S R N V T V N E S E R E F V Y S E R S G L V L T S I L T L R G Q A Q A P P R V I C T A R N L Y G A K S L E L P F Q G A H R L M W A K I G P
- Accession
- P20916
- Protein length
- Fragment
- Predicted molecular weight
- 54.67 kDa
- Actual molecular weight
- 54.67 kDa
- Amino acids
- 20 to 516
- Nature
- Recombinant
Specifications
- Form
- Lyophilized
- Additional notes
SDS-PAGE >= 95%
General info
- Function
Adhesion molecule that mediates interactions between myelinating cells and neurons by binding to neuronal sialic acid-containing gangliosides and to the glycoproteins RTN4R and RTN4RL2 (By similarity). Not required for initial myelination, but seems to play a role in the maintenance of normal axon myelination. Protects motoneurons against apoptosis, also after injury; protection against apoptosis is probably mediated via interaction with neuronal RTN4R and RTN4RL2. Required to prevent degeneration of myelinated axons in adults; this probably depends on binding to gangliosides on the axon cell membrane (By similarity). Negative regulator of neurite outgrowth; in dorsal root ganglion neurons the inhibition is mediated primarily via binding to neuronal RTN4R or RTN4RL2 and to a lesser degree via binding to neuronal gangliosides. In cerebellar granule cells the inhibition is mediated primarily via binding to neuronal gangliosides. In sensory neurons, inhibition of neurite extension depends only partially on RTN4R, RTN4RL2 and gangliosides. Inhibits axon longitudinal growth (By similarity). Inhibits axon outgrowth by binding to RTN4R (By similarity). Preferentially binds to alpha-2,3-linked sialic acid. Binds ganglioside Gt1b (By similarity).
- Sequence similarities
Belongs to the immunoglobulin superfamily. SIGLEC (sialic acid binding Ig-like lectin) family.
- Post-translational modifications
N-glycosylated.
Storage
- Shipped at conditions
- Ambient - Can Ship with Ice
- Appropriate short-term storage conditions
- Ambient
- Appropriate long-term storage conditions
- Ambient
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
MAG (Myelin-Associated Glycoprotein) sometimes referred to as 'MAG protein' 'MAG RAT' or 'MAG/GMA' weighs approximately 100 kDa. This protein is prominently expressed in the central and peripheral nervous systems. MAG localizes to the periaxonal region of myelinated axons making it essential for proper neuronal function. It plays an important role in maintaining the stability and structure of myelin sheaths which are important for the efficient transmission of nerve impulses.
- Biological function summary
This protein acts as an adhesion molecule that contributes to the myelination process. It exists as part of a molecular complex that interacts with other oligodendrocyte and Schwann cell proteins. This interaction is important for nerve regeneration and the expansion of the myelin sheath during development and repair. MAG also assists in signaling between axons and glial cells improving cellular communication and myelin integrity.
- Pathways
MAG significantly impacts pathways such as the Nogo receptor which is associated with inhibiting axonal growth and regeneration. MAG interacts with the NOGO protein and other members of the Nogo receptor signaling pathway where it functions to regulate neuronal growth and regeneration. Furthermore MAG's interplay with NOGO receptor components contributes to limiting excessive sprouting of axons balancing growth and stability of the nervous system.
- Associated diseases and disorders
MAG is notably linked to neuropathies and multiple sclerosis. In neuropathies abnormal MAG expression can disrupt nerve conduction and lead to demyelinating conditions. Multiple sclerosis sees extensive involvement of MAG where its malfunction or reduced expression associates with the failure of remyelination processes. Additionally MAG's connection with proteins such as NOGO and other myelin-associated inhibitors implicates its role in the pathology of these disorders offering potential targets for therapeutic interventions.
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SDS-PAGE - Recombinant Human MAG / GMA Protein (ab290085)
SDS-PAGE analysis of ab290085
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