JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB174430

Recombinant Human MAGED1/NRAGE protein

Be the first to review this product! Submit a review

|

(0 Publication)

Recombinant Human MAGED1/NRAGE protein is a Human Fragment protein, in the 504 to 760 aa range, expressed in Escherichia coli, with >85%, suitable for SDS-PAGE, Mass Spec.

View Alternative Names

NRAGE, PP2250, PRO2292, MAGED1, Melanoma-associated antigen D1, MAGE tumor antigen CCF, MAGE-D1 antigen, Neurotrophin receptor-interacting MAGE homolog

1 Images
SDS-PAGE - Recombinant Human MAGED1/NRAGE protein (AB174430)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant Human MAGED1/NRAGE protein (AB174430)

15% SDS-PAGE analysis of ab174430 (3 μg).

Key facts

Purity

>85% SDS-PAGE

Expression system

Escherichia coli

Tags

His tag N-Terminus

Applications

Mass Spec, SDS-PAGE

applications

Biologically active

No

Accession

Q9Y5V3

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 8 Constituents: 20% Glycerol (glycerin, glycerine), 0.32% Tris HCl, 0.02% (R*,R*)-1,4-Dimercaptobutan-2,3-diol

storage-buffer

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "Mass Spec": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
style
left-column
style
left-column

Product details

This product was previously labelled as MAGED1
style
left-column

Sequence info

[{"sequence":"MGSSHHHHHHSSGLVPRGSHMGSLRPSPNSRASQNPGAAQPRDVALLQERANKLVKYLMLKDYTKVPIKRSEMLRDIIREYTDVYPEIIERACFVLEKKFGIQLKEIDKEEHLYILISTPESLAGILGTTKDTPKLGLLLVILGVIFMNGNRASEAVLWEALRKMGLRPGVRHPLLGDLRKLLTYEFVKQKYLDYRRVPNSNPPEYEFLWGLRSYHETSKMKVLRFIAEVQKRDPRDWTAQFMEAADEALDALDAAAAEAEARAEARTRMGIGDEAVSGP","proteinLength":"Fragment","predictedMolecularWeight":"31.7 kDa","actualMolecularWeight":null,"aminoAcidEnd":760,"aminoAcidStart":504,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"Q9Y5V3","tags":[{"tag":"His","terminus":"N-Terminus"}]}]
style
left-column

Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

MAGED1 also known as NRAGE is a protein with diverse roles in cellular processes. It is a type of MAGE (Melanoma Antigen Gene) family member and its molecular weight is approximately 88 kDa. You find MAGED1 expressed in multiple tissues including the brain and testes where it plays various regulatory roles. This protein associates with other key molecules modulating different signaling events within the cell.
Biological function summary

MAGED1 influences neuronal development by interacting with various cellular components. It acts as a modulator in the NGF (nerve growth factor) and p75 neurotrophin receptor signaling pathways forming a complex with these receptors to manage neuronal apoptosis and differentiation. Its function ensures the appropriate response of neurons to growth signals closely monitoring neuronal growth and survival.

Pathways

MAGED1 is involved in the regulation of apoptosis and neuronal growth signaling. In the apoptosis pathway it partners with p75NTR enhancing the pathway’s pro-apoptotic signals. In neuronal growth signaling it interacts with TrkA and other neurotrophin receptors facilitating the accurate transmission of growth signals. Through these interactions MAGED1 plays a balancing act between promoting cell survival and inducing apoptosis depending on cellular context.

Researchers link MAGED1 to neurodegenerative conditions and cancer. Its involvement in apoptosis and neuronal regulation associates it with Alzheimer’s disease with alterations in MAGED1 expression impacting neuronal cells. Additionally in cancer the imbalance in MAGED1 regulation contributes to tumorigenesis. Its association with proteins like p75NTR and TrkA highlights its role in disease pathways suggesting it potentially serves as a therapeutic target.
style
left-column

Specifications

Form

Liquid

Additional notes

ab174430 is purified by conventional chromatography.

style
left-column

General info

Function

Involved in the apoptotic response after nerve growth factor (NGF) binding in neuronal cells. Inhibits cell cycle progression, and facilitates NGFR-mediated apoptosis. May act as a regulator of the function of DLX family members. May enhance ubiquitin ligase activity of RING-type zinc finger-containing E3 ubiquitin-protein ligases. Proposed to act through recruitment and/or stabilization of the Ubl-conjugating enzyme (E2) at the E3 : substrate complex. Plays a role in the circadian rhythm regulation. May act as RORA co-regulator, modulating the expression of core clock genes such as BMAL1 and NFIL3, induced, or NR1D1, repressed.

Subcellular localisation

Nucleus

style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

Involved in the apoptotic response after nerve growth factor (NGF) binding in neuronal cells. Inhibits cell cycle progression, and facilitates NGFR-mediated apoptosis. May act as a regulator of the function of DLX family members. May enhance ubiquitin ligase activity of RING-type zinc finger-containing E3 ubiquitin-protein ligases. Proposed to act through recruitment and/or stabilization of the Ubl-conjugating enzyme (E2) at the E3 : substrate complex. Plays a role in the circadian rhythm regulation. May act as RORA co-regulator, modulating the expression of core clock genes such as BMAL1 and NFIL3, induced, or NR1D1, repressed.
See full target information MAGED1
style
left-column
style
left-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions
style
left-column
style
right-column
style
bg-slate-100

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com