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AB190407

Recombinant human MAK protein (GST tag N-Terminus)

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Recombinant human MAK protein (GST tag N-Terminus) is a Human Full Length protein, in the 1 to 623 aa range, expressed in Baculovirus infected Sf9 cells, with >75%, suitable for WB, FuncS.

View Alternative Names

Serine/threonine-protein kinase MAK, Male germ cell-associated kinase, MAK

4 Images
Functional Studies - Recombinant human MAK protein (AB190407)
  • FuncS

Unknown

Functional Studies - Recombinant human MAK protein (AB190407)

The specific activity of MAK (ab190407) was determined to be 35 nmol/min/mg as per activity assay protocol

Functional Studies - Recombinant human MAK protein (AB190407)
  • FuncS

Supplier Data

Functional Studies - Recombinant human MAK protein (AB190407)

The specific activity of ab190407 was determined to be 40 nmol/min/mg.

SDS-PAGE - Recombinant human MAK protein (AB190407)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant human MAK protein (AB190407)

The putity of ab190407 was determined to be >75% by densitometry.

SDS-PAGE - Recombinant human MAK protein (AB190407)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant human MAK protein (AB190407)

SDS PAGE analysis of ab190407

Key facts

Purity

>75% SDS-PAGE

Assessed by densitometry. Affinity purified.

Expression system

Baculovirus infected Sf9 cells

Tags

GST tag N-Terminus

Applications

WB, FuncS

applications

Biologically active

Yes

Biological activity

The specific activity of ab190407 was determined to be 40 nmol/min/mg.

Accession

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 7.5 Constituents: 25% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, 0.79% Tris HCl, 0.31% Glutathione, 0.004% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.003% EDTA, 0.002% PMSF

storage-buffer

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Complementary Products

Primary Antibodies

AB279383

Anti-MAK antibody [OTI1G2]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MAK antibody [OTI1G2] (AB279383)

  • 0
  • 0
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "FuncS": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

ab64311 (Myelin Basic Protein protein) can be utilized as a substrate for assessing kinase activity
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Sequence info

[{"linker":null,"sequence":"MNRYTTMRQLGDGTYGSVLMGKSNESGELVAIKRMKRKFYSWDECMNLREVKSLKKLNHANVIKLKEVIRENDHLYFIFEYMKENLYQLMKDRNKLFPESVIRNIMYQILQGLAFIHKHGFFHRDMKPENLLCMGPELVKIADFGLARELRSQPPYTDYVSTRWYRAPEVLLRSSVYSSPIDVWAVGSIMAELYMLRPLFPGTSEVDEIFKICQVLGTPKKSDWPEGYQLASSMNFRFPQCVPINLKTLIPNASNEAIQLMTEMLNWDPKKRPTASQALKHPYFQVGQVLGPSSNHLESKQSLNKQLQPLESKPSLVEVEPKPLPDIIDQVVGQPQPKTSQQPLQPIQPPQNLSVQQPPKQQSQEKPPQTLFPSIVKNMPTKPNGTLSHKSGRRRWGQTIFKSGDSWEELEDYDFGASHSKKPSMGVFKEKRKKDSPFRLPEPVPSGSNHSTGENKSLPAVTSLKSDSELSTAPTSKQYYLKQSRYLPGVNPKKVSLIASGKEINPHTWSNQLFPKSLGPVGAELAFKRSNAGNLGSYATYNQSGYIPSFLKKEVQSAGQRIHLAPLNATASEYTWNTKTGRGQFSGRTYNPTAKNLNIVNRAQPIPSVHGRTDWVAKYGGHR","proteinLength":"Full Length","predictedMolecularWeight":"78 kDa","actualMolecularWeight":null,"aminoAcidEnd":623,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Baculovirus infected Sf9 cells","accessionNumber":"P20794","tags":[{"tag":"GST","terminus":"N-Terminus"}]}]
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification
Shipped at conditions
Dry Ice
Appropriate short-term storage conditions
-80°C
Appropriate long-term storage conditions
-80°C
Storage information
Avoid freeze / thaw cycle
True
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General info

Function

Essential for the regulation of ciliary length and required for the long-term survival of photoreceptors (By similarity). Phosphorylates FZR1 in a cell cycle-dependent manner. Plays a role in the transcriptional coactivation of AR. Could play an important function in spermatogenesis. May play a role in chromosomal stability in prostate cancer cells.

Sequence similarities

Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily.

Post-translational modifications

Autophosphorylated. Phosphorylated on serine and threonine residues.

Subcellular localisation

Nucleus

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Product protocols

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Target data

Essential for the regulation of ciliary length and required for the long-term survival of photoreceptors (By similarity). Phosphorylates FZR1 in a cell cycle-dependent manner. Plays a role in the transcriptional coactivation of AR. Could play an important function in spermatogenesis. May play a role in chromosomal stability in prostate cancer cells.
See full target information MAK
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