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AB167910

Recombinant Human MAK3 protein (His tag N-Terminus)

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Recombinant Human MAK3 protein (His tag N-Terminus) is a Human Full Length protein, in the 1 to 362 aa range, expressed in Escherichia coli, with >90%, suitable for SDS-PAGE, Mass Spec.

View Alternative Names

C14orf35, MAK3, NAT12, NAA30, N-alpha-acetyltransferase 30, N-acetyltransferase 12, N-acetyltransferase MAK3 homolog, NatC catalytic subunit

1 Images
SDS-PAGE - Recombinant Human MAK3 protein (His tag N-Terminus) (AB167910)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant Human MAK3 protein (His tag N-Terminus) (AB167910)

15% SDS-PAGE analysis of 3 μg ab167910.

Key facts

Purity

>90% SDS-PAGE

Expression system

Escherichia coli

Tags

His tag N-Terminus

Applications

SDS-PAGE, Mass Spec

applications

Biologically active

No

Accession

Q147X3

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 8 Constituents: 10% Glycerol (glycerin, glycerine), 0.88% Sodium chloride, 0.32% Tris HCl, 0.01% (R*,R*)-1,4-Dimercaptobutan-2,3-diol

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "Mass Spec": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Sequence info

[{"sequence":"MGSSHHHHHHSSGLVPRGSHMGSMAEVPPGPSSLLPPPAPPAPAAVEPRCPFPAGAALACCSEDEEDDEEHEGGGSRSPAGGESATVAAKGHPCLRCPQPPQEQQQLNGLISPELRHLRAAASLKSKVLSVAEVAATTATPDGGPRATATKGAGVHSGERPPHSLSSNARTAVPSPVEAAAASDPAAARNGLAEGTEQEEEEEDEQVRLLSSSLTADCSLRSPSGREVEPGEDRTIRYVRYESELQMPDIMRLITKDLSEPYSIYTYRYFIHNWPQLCFLAMVGEECVGAIVCKLDMHKKMFRRGYIAMLAVDSKYRRNGIGTNLVKKAIYAMVEGDCDEVVLETEITNKSALKLYENLGFVRDKRLFRYYLNGVDALRLKLWLR","proteinLength":"Full Length","predictedMolecularWeight":"41.7 kDa","actualMolecularWeight":null,"aminoAcidEnd":362,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"Q147X3","tags":[{"tag":"His","terminus":"N-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
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Specifications

Form

Liquid

Additional notes

ab167910 is purified by conventional chromatography techniques.

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General info

Function

Catalytic subunit of the N-terminal acetyltransferase C (NatC) complex (PubMed : 19398576, PubMed : 37891180). Catalyzes acetylation of the N-terminal methionine residues of peptides beginning with Met-Leu-Ala and Met-Leu-Gly (PubMed : 19398576, PubMed : 37891180). N-terminal acetylation protects proteins from ubiquitination and degradation by the N-end rule pathway (PubMed : 37891180). Necessary for the lysosomal localization and function of ARL8B sugeesting that ARL8B is a NatC substrate (PubMed : 19398576).

Sequence similarities

Belongs to the acetyltransferase family. MAK3 subfamily.

Subcellular localisation

Nucleus

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Product protocols

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Target data

Catalytic subunit of the N-terminal acetyltransferase C (NatC) complex (PubMed : 19398576, PubMed : 37891180). Catalyzes acetylation of the N-terminal methionine residues of peptides beginning with Met-Leu-Ala and Met-Leu-Gly (PubMed : 19398576, PubMed : 37891180). N-terminal acetylation protects proteins from ubiquitination and degradation by the N-end rule pathway (PubMed : 37891180). Necessary for the lysosomal localization and function of ARL8B sugeesting that ARL8B is a NatC substrate (PubMed : 19398576).
See full target information NAA30
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