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Recombinant Human Mannan Binding Lectin/MBL protein (His tag) is a Human Full Length protein, in the 21 to 248 aa range, expressed in Baculovirus infected insect, with >90% purity, < 1 EU/µg endotoxin level and suitable for SDS-PAGE.

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Images

SDS-PAGE - Recombinant Human Mannan Binding Lectin/MBL protein (His tag) (AB229367), expandable thumbnail

Key facts

Purity

>90% SDS-PAGE

Endotoxin level

< 1 EU/µg

Expression system

Baculovirus infected insect cells

Tags

His tag C-Terminus

Applications

SDS-PAGE

Biologically active

No

Amino acid sequence

A D P E T V T C E D A Q K T C P A V I A C S S P G I N G F P G K D G R D G T K G E K G E P G Q G L R G L Q G P P G K L G P P G N P G P S G S P G P K G Q K G D P G K S P D G D S S L A A S E R K A L Q T E M A R I K K W L T F S L G K Q V G N K F F L T N G E I M T F E K V K A L C V K F Q A S V A T P R N A A E N G A I Q N L I K E E A F L G I T D E K T E G Q F V D L T G N R L T Y T N W N E G E P N N A G S D E D C V L L L K N G Q W N D V P C S T S H L A V C E F P I H H H H H H

Reactivity data

Application

SDS-PAGE

Reactivity

Reacts

Dilution info

-

Notes

Migrates as 28-40 kDa under reducing conditions.

Associated Products

Select an associated product type

2 products for Alternative Product

Target data

Function

Calcium-dependent lectin involved in innate immune defense (PubMed:35102342). Binds mannose, fucose and N-acetylglucosamine on different microorganisms and activates the lectin complement pathway. Binds to late apoptotic cells, as well as to apoptotic blebs and to necrotic cells, but not to early apoptotic cells, facilitating their uptake by macrophages. May bind DNA. Upon SARS coronavirus-2/SARS-CoV-2 infection, activates the complement lectin pathway which leads to the inhibition SARS-CoV-2 infection and a reduction of the induced inflammatory response (PubMed:35102342).

Alternative names

Recommended products

Recombinant Human Mannan Binding Lectin/MBL protein (His tag) is a Human Full Length protein, in the 21 to 248 aa range, expressed in Baculovirus infected insect, with >90% purity, < 1 EU/µg endotoxin level and suitable for SDS-PAGE.

Key facts

Purity

>90% SDS-PAGE

Endotoxin level

< 1 EU/µg

Expression system

Baculovirus infected insect cells

Applications

SDS-PAGE

Accession
P11226-1
Animal free

No

Species

Human

Concentration
Loading...
Storage buffer

pH: 7.4
Constituents: PBS, 10% Glycerol (glycerin, glycerine)

Sequence info

Amino acid sequence

A D P E T V T C E D A Q K T C P A V I A C S S P G I N G F P G K D G R D G T K G E K G E P G Q G L R G L Q G P P G K L G P P G N P G P S G S P G P K G Q K G D P G K S P D G D S S L A A S E R K A L Q T E M A R I K K W L T F S L G K Q V G N K F F L T N G E I M T F E K V K A L C V K F Q A S V A T P R N A A E N G A I Q N L I K E E A F L G I T D E K T E G Q F V D L T G N R L T Y T N W N E G E P N N A G S D E D C V L L L K N G Q W N D V P C S T S H L A V C E F P I H H H H H H

Accession

P11226

Protein length

Full Length

Predicted molecular weight

25.1 kDa

Amino acids

21 to 248

Nature

Recombinant

Tags

His tag C-Terminus

Specifications

Form

Liquid

Additional notes

Affinity purified

General info

Function

Calcium-dependent lectin involved in innate immune defense (PubMed:35102342). Binds mannose, fucose and N-acetylglucosamine on different microorganisms and activates the lectin complement pathway. Binds to late apoptotic cells, as well as to apoptotic blebs and to necrotic cells, but not to early apoptotic cells, facilitating their uptake by macrophages. May bind DNA. Upon SARS coronavirus-2/SARS-CoV-2 infection, activates the complement lectin pathway which leads to the inhibition SARS-CoV-2 infection and a reduction of the induced inflammatory response (PubMed:35102342).

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

This product was previously labelled as Mannan Binding Lectin

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

Mannan Binding Lectin often referred to as MBL is an important player in the innate immune system. It is known alternatively as mannose-binding lectin or mannan-binding protein. The mass of MBL is approximately 32 to 34 kilodaltons and it is primarily produced in the liver. MBL circulates in the bloodstream where it can recognize patterns on the surface of pathogens such as bacteria and viruses through its lectin-binding domain. This recognition leads to the activation of other components that play roles in immune response.

Biological function summary

Mannan Binding Lectin serves a significant role in immune defense by binding to carbohydrate structures on pathogens. It is part of a complex known as the lectin pathway of the complement system one of three pathways that activate the complement system to enhance pathogen removal. MBL can activate through a series of proteases leading to opsonization cell lysis and inflammation which are vital responses to infection.

Pathways

Mannan recognition by MBL initiates the lectin pathway an essential component of the complement system. This pathway converges with the classical and alternative pathways to enhance the immune response. MBL works closely with MBL-associated serine proteases (MASPs) particularly MASP-1 and MASP-2 which cleave complement proteins resulting in pathogen opsonization and membrane attack complex formation. These actions are tightly regulated and ensure effective defense against invaders.

Associated diseases and disorders

Deficiencies or polymorphisms in MBL can lead to increased susceptibility to infections and developmental complications of the immune system. Individuals with low MBL levels may experience recurrent respiratory infections or other bacterial infections due to inadequate complement activation. Moreover variations in the MBL protein have connections with autoimmune diseases such as systemic lupus erythematosus where MBL levels might influence disease severity. In these conditions altered interactions with other immune proteins like C4 and C3 may contribute to pathological responses.

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1 product image

  • SDS-PAGE - Recombinant Human Mannan Binding Lectin/MBL protein (His tag) (ab229367), expandable thumbnail

    SDS-PAGE - Recombinant Human Mannan Binding Lectin/MBL protein (His tag) (ab229367)

    15% SDS-PAGE analysis of 3 μg Recombinant Human Mannan Binding Lectin/MBL protein (His tag) (ab229367).

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Product protocols

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