Recombinant Human Mannan Binding Lectin/MBL protein (His tag) is a Human Full Length protein, in the 21 to 248 aa range, expressed in Baculovirus infected insect, with >90% purity, < 1 EU/µg endotoxin level and suitable for SDS-PAGE.
>90% SDS-PAGE
< 1 EU/µg
Baculovirus infected insect cells
His tag C-Terminus
SDS-PAGE
No
A D P E T V T C E D A Q K T C P A V I A C S S P G I N G F P G K D G R D G T K G E K G E P G Q G L R G L Q G P P G K L G P P G N P G P S G S P G P K G Q K G D P G K S P D G D S S L A A S E R K A L Q T E M A R I K K W L T F S L G K Q V G N K F F L T N G E I M T F E K V K A L C V K F Q A S V A T P R N A A E N G A I Q N L I K E E A F L G I T D E K T E G Q F V D L T G N R L T Y T N W N E G E P N N A G S D E D C V L L L K N G Q W N D V P C S T S H L A V C E F P I H H H H H H
Application | Reactivity | Dilution info | Notes |
---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes Migrates as 28-40 kDa under reducing conditions. |
Select an associated product type
Calcium-dependent lectin involved in innate immune defense (PubMed:35102342). Binds mannose, fucose and N-acetylglucosamine on different microorganisms and activates the lectin complement pathway. Binds to late apoptotic cells, as well as to apoptotic blebs and to necrotic cells, but not to early apoptotic cells, facilitating their uptake by macrophages. May bind DNA. Upon SARS coronavirus-2/SARS-CoV-2 infection, activates the complement lectin pathway which leads to the inhibition SARS-CoV-2 infection and a reduction of the induced inflammatory response (PubMed:35102342).
COLEC1, MBL, MBL, COLEC1, MBL2, Mannose-binding protein C, MBP-C, Collectin-1, MBP1, Mannan-binding protein, Mannose-binding lectin
Recombinant Human Mannan Binding Lectin/MBL protein (His tag) is a Human Full Length protein, in the 21 to 248 aa range, expressed in Baculovirus infected insect, with >90% purity, < 1 EU/µg endotoxin level and suitable for SDS-PAGE.
>90% SDS-PAGE
< 1 EU/µg
Baculovirus infected insect cells
His tag C-Terminus
SDS-PAGE
No
No
Human
pH: 7.4
Constituents: PBS, 10% Glycerol (glycerin, glycerine)
A D P E T V T C E D A Q K T C P A V I A C S S P G I N G F P G K D G R D G T K G E K G E P G Q G L R G L Q G P P G K L G P P G N P G P S G S P G P K G Q K G D P G K S P D G D S S L A A S E R K A L Q T E M A R I K K W L T F S L G K Q V G N K F F L T N G E I M T F E K V K A L C V K F Q A S V A T P R N A A E N G A I Q N L I K E E A F L G I T D E K T E G Q F V D L T G N R L T Y T N W N E G E P N N A G S D E D C V L L L K N G Q W N D V P C S T S H L A V C E F P I H H H H H H
Full Length
25.1 kDa
21 to 248
Recombinant
His tag C-Terminus
Liquid
Affinity purified
Calcium-dependent lectin involved in innate immune defense (PubMed:35102342). Binds mannose, fucose and N-acetylglucosamine on different microorganisms and activates the lectin complement pathway. Binds to late apoptotic cells, as well as to apoptotic blebs and to necrotic cells, but not to early apoptotic cells, facilitating their uptake by macrophages. May bind DNA. Upon SARS coronavirus-2/SARS-CoV-2 infection, activates the complement lectin pathway which leads to the inhibition SARS-CoV-2 infection and a reduction of the induced inflammatory response (PubMed:35102342).
Blue Ice
1-2 weeks
+4°C
-20°C
Upon delivery aliquot
Avoid freeze / thaw cycle
This product was previously labelled as Mannan Binding Lectin
This supplementary information is collated from multiple sources and compiled automatically.
Mannan Binding Lectin often referred to as MBL is an important player in the innate immune system. It is known alternatively as mannose-binding lectin or mannan-binding protein. The mass of MBL is approximately 32 to 34 kilodaltons and it is primarily produced in the liver. MBL circulates in the bloodstream where it can recognize patterns on the surface of pathogens such as bacteria and viruses through its lectin-binding domain. This recognition leads to the activation of other components that play roles in immune response.
Mannan Binding Lectin serves a significant role in immune defense by binding to carbohydrate structures on pathogens. It is part of a complex known as the lectin pathway of the complement system one of three pathways that activate the complement system to enhance pathogen removal. MBL can activate through a series of proteases leading to opsonization cell lysis and inflammation which are vital responses to infection.
Mannan recognition by MBL initiates the lectin pathway an essential component of the complement system. This pathway converges with the classical and alternative pathways to enhance the immune response. MBL works closely with MBL-associated serine proteases (MASPs) particularly MASP-1 and MASP-2 which cleave complement proteins resulting in pathogen opsonization and membrane attack complex formation. These actions are tightly regulated and ensure effective defense against invaders.
Deficiencies or polymorphisms in MBL can lead to increased susceptibility to infections and developmental complications of the immune system. Individuals with low MBL levels may experience recurrent respiratory infections or other bacterial infections due to inadequate complement activation. Moreover variations in the MBL protein have connections with autoimmune diseases such as systemic lupus erythematosus where MBL levels might influence disease severity. In these conditions altered interactions with other immune proteins like C4 and C3 may contribute to pathological responses.
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15% SDS-PAGE analysis of 3 μg Recombinant Human Mannan Binding Lectin/MBL protein (His tag) (ab229367).
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