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AB111629

Recombinant Human Mannose Phosphate Isomerase protein (denatured)

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Recombinant Human Mannose Phosphate Isomerase protein (denatured) is a Human Full Length protein, in the 1 to 362 aa range, expressed in Escherichia coli, with >90%, suitable for SDS-PAGE.

View Alternative Names

PMI1, MPI, Mannose-6-phosphate isomerase, Phosphohexomutase, Phosphomannose isomerase, PMI

1 Images
SDS-PAGE - Recombinant Human Mannose Phosphate Isomerase protein (denatured) (AB111629)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant Human Mannose Phosphate Isomerase protein (denatured) (AB111629)

15% SDS-PAGE showing ab111629 at approximately 41.9kDa (3μg).

Key facts

Purity

>90% SDS-PAGE

Expression system

Escherichia coli

Tags

His tag N-Terminus

Applications

SDS-PAGE

applications

Biologically active

No

Accession

P34949

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 8 Constituents: 2.4% Urea, 0.32% Tris HCl

storage-buffer

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

Sequence info

[{"sequence":"MGSSHHHHHHSSGLVPRGSHMAAPRVFPLSCAVQQYAWGKMGSNSEVARLLASSDPLAQIAEDKPYAELWMGTHPRGDAKILDNRISQKTLSQWIAENQDSLGSKVKDTFNGNLPFLFKVLSVETPLSIQAHPNKELAEKLHLQAPQHYPDANHKPEMAIALTPFQGLCGFRPVEEIVTFLKTAAGNNMEDIFGELLLQLHQQYPGDIGCFAIYFLNLLTLKPGEAMFLEANVPHAYLKGDCVECMACSDNTVRAGLTPKFIDVPTLCEMLSYTPSSSKDRLFLPTRSQEDPYLSIYDPPVPDFTIMKTEVPGSVTEYKVLALDSASILLMVQGTVIASTPTTQTPIPLQRGGVLFIGANESVSLKLTEPKDLLIFRACCLL","proteinLength":"Full Length","predictedMolecularWeight":"41.9 kDa","actualMolecularWeight":null,"aminoAcidEnd":362,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"P34949","tags":[{"tag":"His","terminus":"N-Terminus"}]}]

Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Mannose Phosphate Isomerase also known as PMI is an enzyme that catalyzes the interconversion of fructose-6-phosphate and mannose-6-phosphate. This enzyme with a mass of approximately 42 kDa plays an important role in the metabolism of mannose a type of sugar. Expression of Mannose Phosphate Isomerase occurs in many tissues including liver muscle and fibroblasts where it helps maintain the balance of sugar phosphates needed for various cellular functions.
Biological function summary

This enzyme facilitates the processing of mannose through the biosynthesis pathways of glycoproteins and glycolipids. Mannose Phosphate Isomerase is not part of a larger protein complex but it functions independently to execute its role within the cell. It ensures that cells have adequate mannose-6-phosphate which is essential for proper glycosylation a process necessary for protein folding and stability.

Pathways

Mannose Phosphate Isomerase influences the hexosamine biosynthesis pathway and glycolysis. In the hexosamine biosynthesis pathway it works closely with enzymes such as phosphomannomutase to provide critical components for the production of glycosaminoglycans and glycoproteins. In glycolysis it helps integrate mannose into energy production processes by providing intermediates necessary for glucose metabolism.

Defects in Mannose Phosphate Isomerase can lead to conditions such as congenital disorder of glycosylation type Ib and hypoglycemia-related disorders. This enzyme's malfunction disrupts normal glycosylation which is important for protein function and stability linking it to glycosylation disorders. Connections also exist with phosphomannomutase another enzyme affected in similar glycosylation disorders highlighting its relevance in disease pathology.

Specifications

Form

Liquid

General info

Function

Isomerase that catalyzes the interconversion of fructose-6-P and mannose-6-P and has a critical role in the supply of D-mannose derivatives required for many eukaryotic glycosylation reactions.

Sequence similarities

Belongs to the mannose-6-phosphate isomerase type 1 family.

Product protocols

Target data

Isomerase that catalyzes the interconversion of fructose-6-P and mannose-6-P and has a critical role in the supply of D-mannose derivatives required for many eukaryotic glycosylation reactions.
See full target information MPI

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