Recombinant Human MAP1 protein (denatured) is a Human Full Length protein, in the 1 to 351 aa range, expressed in Escherichia coli, with >85% purity and suitable for SDS-PAGE.
Images
Key facts
- Purity
- >85% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE
- Biologically active
- No
Amino acid sequence
M T L R L L E D W C R G M D M N P R K A L L I A G I S Q S C S V A E I E E A L Q A G L A P L G E Y R L L G R M F R R D E N R K V A L V G L T A E T S H A L V P K E I P G K G G I W R V I F K P P D P D N T F L S R L N E F L A G E G M T V G E L S R A L G H E N G S L D P E Q G M I P E M W A P M L A Q A L E A L Q P A L Q C L K Y K K L R V F S G R E S P E P G E E E F G R W M F H T T Q M I K A W Q V P D V E K R R R L L E S L R G P A L D V I R V L K I N N P L I T V D E C L Q A L E E V F G V T D N P R E L Q V K Y L T T Y Q K D E E K L S A Y V L R L E P L L Q K L V Q R G A I E R D A V N Q A R L D Q V I A G A V H K T I R R E L N L P E D G P A P G F L Q L L V L I K D Y E A A E E E E A L L Q A I L E G N F T
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Retrotransposon-derived protein that forms virion-like capsids (By similarity). Acts as an effector of BAX during apoptosis: enriched at outer mitochondria membrane and associates with BAX upon induction of apoptosis, facilitating BAX-dependent mitochondrial outer membrane permeabilization and apoptosis (PubMed:11060313, PubMed:16199525). Required for death receptor-dependent apoptosis (PubMed:11060313). When associated with RASSF1, promotes BAX conformational change and translocation to mitochondrial membranes in response to TNF and TNFSF10 stimulation (PubMed:15949439). Also promotes autophagy: promotes phagophore closure via association with ATG8 proteins (PubMed:33783314). Acts as an inhibitor of the NFE2L2/NRF2 pathway via interaction with SQSTM1: interaction promotes dissociation of SQSTM1 inclusion bodies that sequester KEAP1, relieving inactivation of the BCR(KEAP1) complex (PubMed:33393215).
Alternative names
PNMA4, MOAP1, Modulator of apoptosis 1, MAP-1, MAP1, Paraneoplastic antigen Ma4
Recommended products
Recombinant Human MAP1 protein (denatured) is a Human Full Length protein, in the 1 to 351 aa range, expressed in Escherichia coli, with >85% purity and suitable for SDS-PAGE.
Key facts
- Purity
- >85% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE
- Biologically active
- No
- Accession
- Q96BY2-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 8
Constituents: 10% Glycerol (glycerin, glycerine), 2.4% Urea, 0.32% Tris-HCl buffer
Sequence info
Amino acid sequence
- M T L R L L E D W C R G M D M N P R K A L L I A G I S Q S C S V A E I E E A L Q A G L A P L G E Y R L L G R M F R R D E N R K V A L V G L T A E T S H A L V P K E I P G K G G I W R V I F K P P D P D N T F L S R L N E F L A G E G M T V G E L S R A L G H E N G S L D P E Q G M I P E M W A P M L A Q A L E A L Q P A L Q C L K Y K K L R V F S G R E S P E P G E E E F G R W M F H T T Q M I K A W Q V P D V E K R R R L L E S L R G P A L D V I R V L K I N N P L I T V D E C L Q A L E E V F G V T D N P R E L Q V K Y L T T Y Q K D E E K L S A Y V L R L E P L L Q K L V Q R G A I E R D A V N Q A R L D Q V I A G A V H K T I R R E L N L P E D G P A P G F L Q L L V L I K D Y E A A E E E E A L L Q A I L E G N F T
- Accession
- Q96BY2
- Protein length
- Full Length
- Predicted molecular weight
- 41.9 kDa
- Amino acids
- 1 to 351
- Nature
- Recombinant
- Tags
- His tag N-Terminus
Specifications
- Form
- Liquid
General info
- Function
Retrotransposon-derived protein that forms virion-like capsids (By similarity). Acts as an effector of BAX during apoptosis: enriched at outer mitochondria membrane and associates with BAX upon induction of apoptosis, facilitating BAX-dependent mitochondrial outer membrane permeabilization and apoptosis (PubMed:11060313, PubMed:16199525). Required for death receptor-dependent apoptosis (PubMed:11060313). When associated with RASSF1, promotes BAX conformational change and translocation to mitochondrial membranes in response to TNF and TNFSF10 stimulation (PubMed:15949439). Also promotes autophagy: promotes phagophore closure via association with ATG8 proteins (PubMed:33783314). Acts as an inhibitor of the NFE2L2/NRF2 pathway via interaction with SQSTM1: interaction promotes dissociation of SQSTM1 inclusion bodies that sequester KEAP1, relieving inactivation of the BCR(KEAP1) complex (PubMed:33393215).
- Sequence similarities
Belongs to the PNMA family.
- Post-translational modifications
Ubiquitinated and degraded during mitotic exit by APC/C-Cdh1, this modification is inhibited by TRIM39.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Product promise
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1 product image
SDS-PAGE - Recombinant Human MAP1 protein (denatured) (ab176051)
15% SDS-PAGE analysis of ab176051 (3μg).
Downloads
Product protocols
- Visit the general protocols
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