Recombinant Human MAP1D protein - BSA and Azide free is a Human Full Length protein, in the 20 to 335 aa range, expressed in Escherichia coli, with >85% purity and suitable for MS, SDS-PAGE.
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Key facts
- Purity
- >85% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- His tag N-Terminus
- Applications
- MS, SDS-PAGE
- Biologically active
- No
Amino acid sequence
M G S S H H H H H H S S G L V P R G S H M G S S S P L N H I Y L H K Q S S S Q Q R R N F F F R R Q R D I S H S I V L P A A V S S A H P V P K H I K K P D Y V T T G I V P D W G D S I E V K N E D Q I Q G L H Q A C Q L A R H V L L L A G K S L K V D M T T E E I D A L V H R E I I S H N A Y P S P L G Y G G F P K S V C T S V N N V L C H G I P D S R P L Q D G D I I N I D V T V Y Y N G Y H G D T S E T F L V G N V D E C G K K L V E V A R R C R D E A I A A C R A G A P F S V I G N T I S H I T H Q N G F Q V C P H F V G H G I G S Y F H G H P E I W H H A N D S D L P M E E G M A F T I E P I I T E G S P E F K V L E D A W T V V S L D N Q R S A Q F E H T V L I T S R G A Q I L T K L P H E A
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application MS | Reactivity Reacts | Dilution info - | Notes - |
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes Use at 3 μg/lane |
Target data
Function
Removes the N-terminal methionine from nascent proteins. The N-terminal methionine is often cleaved when the second residue in the primary sequence is small and uncharged (Met-Ala-, Cys, Gly, Pro, Ser, Thr, or Val). Requires deformylation of the N(alpha)-formylated initiator methionine before it can be hydrolyzed (By similarity). May play a role in colon tumorigenesis.
Alternative names
MAP1D, METAP1D, MAP 1D, MetAP 1D, Peptidase M 1D
Recommended products
Recombinant Human MAP1D protein - BSA and Azide free is a Human Full Length protein, in the 20 to 335 aa range, expressed in Escherichia coli, with >85% purity and suitable for MS, SDS-PAGE.
Key facts
- Purity
- >85% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- His tag N-Terminus
- Applications
- MS, SDS-PAGE
- Biologically active
- No
- Accession
- Q6UB28-1
- Animal free
- No
- Carrier free
- Yes
- Species
- Human
- Concentration
- Storage buffer
pH: 8
Constituents: 20% Glycerol (glycerin, glycerine), 0.58% Sodium chloride, 0.32% Tris-HCl buffer, 0.02% (R*,R*)-1,4-Dimercaptobutan-2,3-diol
Sequence info
Amino acid sequence
- M G S S H H H H H H S S G L V P R G S H M G S S S P L N H I Y L H K Q S S S Q Q R R N F F F R R Q R D I S H S I V L P A A V S S A H P V P K H I K K P D Y V T T G I V P D W G D S I E V K N E D Q I Q G L H Q A C Q L A R H V L L L A G K S L K V D M T T E E I D A L V H R E I I S H N A Y P S P L G Y G G F P K S V C T S V N N V L C H G I P D S R P L Q D G D I I N I D V T V Y Y N G Y H G D T S E T F L V G N V D E C G K K L V E V A R R C R D E A I A A C R A G A P F S V I G N T I S H I T H Q N G F Q V C P H F V G H G I G S Y F H G H P E I W H H A N D S D L P M E E G M A F T I E P I I T E G S P E F K V L E D A W T V V S L D N Q R S A Q F E H T V L I T S R G A Q I L T K L P H E A
- Accession
- Q6UB28
- Protein length
- Full Length
- Predicted molecular weight
- 37.4 kDa
- Amino acids
- 20 to 335
- Nature
- Recombinant
- Tags
- His tag N-Terminus
Specifications
- Form
- Liquid
- Additional notes
ab174389 is purified using anion-exchange chromatography (DEAE sepharose resin) and gel-filtration chromatography (Sephacryl S-200) with 20 mM Tris pH 7.5, 2 mM EDTA.
General info
- Function
Removes the N-terminal methionine from nascent proteins. The N-terminal methionine is often cleaved when the second residue in the primary sequence is small and uncharged (Met-Ala-, Cys, Gly, Pro, Ser, Thr, or Val). Requires deformylation of the N(alpha)-formylated initiator methionine before it can be hydrolyzed (By similarity). May play a role in colon tumorigenesis.
- Sequence similarities
Belongs to the peptidase M24A family. Methionine aminopeptidase type 1 subfamily.
- Subcellular localisation
- Mitochondrion
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Product promise
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Full details and terms and conditions can be found here:
Terms & Conditions.
1 product image
SDS-PAGE - Recombinant Human MAP1D protein - BSA and Azide free (ab174389)
SDS-PAGE analysis MAP1D using 3 μg of ab174389 on a 15% gel.
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Product protocols
- Visit the general protocols
- Visit troubleshooting
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