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AB104027

Recombinant human MAP3K1 protein

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Recombinant human MAP3K1 protein is a Human Fragment protein, in the 664 to 1512 aa range, expressed in Baculovirus infected Sf9 cells, with >75%, suitable for SDS-PAGE, FuncS.

View Alternative Names

MAPKKK1, MEKK, MEKK1, MAP3K1, Mitogen-activated protein kinase kinase kinase 1, MAPK/ERK kinase kinase 1, MEK kinase 1, MEKK 1

4 Images
Functional Studies - Recombinant human MAP3K1 protein (AB104027)
  • FuncS

Unknown

Functional Studies - Recombinant human MAP3K1 protein (AB104027)

Kinase Assay demonstrating specific activity of ab104027.

Functional Studies - Recombinant human MAP3K1 protein (AB104027)
  • FuncS

Unknown

Functional Studies - Recombinant human MAP3K1 protein (AB104027)

The specific activity of MAP3K1 (ab104027) was determined to be 8.5 nmol/min/mg as per activity assay protocol and was equivalent to 820 nmol/min/mg as per radiometric assay

SDS-PAGE - Recombinant human MAP3K1 protein (AB104027)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant human MAP3K1 protein (AB104027)

SDS-PAGE showing ab104027 at approximately 155kDa.

SDS-PAGE - Recombinant human MAP3K1 protein (AB104027)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant human MAP3K1 protein (AB104027)

SDS PAGE analysis of ab104027

Key facts

Purity

>75% SDS-PAGE

Purity was determined to be >75% by densitometry. Affinity purified.

Expression system

Baculovirus infected Sf9 cells

Tags

GST tag N-Terminus

Applications

FuncS, SDS-PAGE

applications

Biologically active

Yes

Biological activity

The Specific activity of ab104027 was determined to be 820 nmol/min/mg.

Accession

Q13233

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 7.5 Constituents: 25% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, 0.79% Tris HCl, 0.307% Glutathione, 0.01% Triton X-100, 0.00385% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.00292% EDTA, 0.00174% PMSF

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Complementary Products

Primary Antibodies

AB33912

Anti-MEK4/MKK4 antibody [EP615Y]

RabMAb|Recombinant|KO Validated

Western blot - Anti-MEK4/MKK4 antibody [EP615Y] (AB33912)

  • 3
  • 1
Primary Antibodies

AB33918

Anti-MEKK2 antibody [EP626Y]

RabMAb|Recombinant|KO Validated

Immunocytochemistry/ Immunofluorescence - Anti-MEKK2 antibody [EP626Y] (AB33918)

  • 5
  • 2
Primary Antibodies

AB138662

Anti-MAP3K1 (phospho T1400) antibody

Western blot - Anti-MAP3K1 (phospho T1400) antibody (AB138662)

  • 4
  • 3
Proteins & Peptides

AB125643

Recombinant human p38 delta/MAPK13 protein

Functional Studies - Recombinant human p38 delta/MAPK13 protein (AB125643)

  • 0
  • 0
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "FuncS": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

ab126919 (Human MEK1 full length protein), ab126906 (Human ERK1 full length protein) and ab64311 (Myelin Basic Protein protein) can be utilized as a substrate for assessing kinase activity

This product was previously labelled as MEKK1

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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Sequence info

[{"linker":null,"sequence":"","proteinLength":"Fragment","predictedMolecularWeight":"155 kDa","actualMolecularWeight":null,"aminoAcidEnd":1512,"aminoAcidStart":664,"nature":"Recombinant","expressionSystem":null,"accessionNumber":"Q13233","tags":[{"tag":"GST","terminus":"N-Terminus"}]}]
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification
Shipped at conditions
Dry Ice
Appropriate short-term storage conditions
-80°C
Appropriate long-term storage conditions
-80°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
True
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General info

Function

Component of a protein kinase signal transduction cascade (PubMed : 9808624). Activates the ERK and JNK kinase pathways by phosphorylation of MAP2K1 and MAP2K4 (PubMed : 9808624). May phosphorylate the MAPK8/JNK1 kinase (PubMed : 17761173). Activates CHUK and IKBKB, the central protein kinases of the NF-kappa-B pathway (PubMed : 9808624).

Sequence similarities

Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase kinase subfamily.

Post-translational modifications

Autophosphorylated.

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Product protocols

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Target data

Component of a protein kinase signal transduction cascade (PubMed : 9808624). Activates the ERK and JNK kinase pathways by phosphorylation of MAP2K1 and MAP2K4 (PubMed : 9808624). May phosphorylate the MAPK8/JNK1 kinase (PubMed : 17761173). Activates CHUK and IKBKB, the central protein kinases of the NF-kappa-B pathway (PubMed : 9808624).
See full target information MAP3K1
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Product promise

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