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Recombinant human MAP3K8/COT protein is a Human Fragment protein, in the 30 to 397 aa range, expressed in Baculovirus infected Sf9, with >75% purity and suitable for SDS-PAGE, FuncS.
Alternative names=Mitogen-activated protein kinase kinase kinase 8, Cancer Osaka thyroid oncogene, Proto-oncogene c-Cot, Serine/threonine-protein kinase cot, Tumor progression locus 2, TPL-2, ESTF, COT, MAP3K8
- Purity
>75% Densitometry
- Expression system
Baculovirus infected Sf9 cells
- Tags
GST tag N-Terminus
- Applications
SDS-PAGE, FuncS
- Biologically active
Yes
- Sequence
M E N L Y A S E E P A V Y E P S L M T M C Q D S N Q N D E R S K S L L L S G Q E V P W L S S V R Y G T V E D L L A F A N H I S N T A K H F Y G Q R P Q E S G I L L N M V I T P Q N G R Y Q I D S D V L L I P W K L T Y R N I G S D F I P R G A F G K V Y L A Q D I K T K K R M A C K L I P V D Q F K P S D V E I Q A C F R H E N I A E L Y G A V L W G E T V H L F M E A G E G G S V L E K L E S C G P M R E F E I I W V T K H V L K G L D F L H S K K V I H H D I K P S N I V F M S T K A V L V D F G L S V Q M T E D V Y F P K D L R G T E I Y M S P E V I L C R G H S T K A D I Y S L G A T L I H M Q T G T P P W V K R Y P R S A Y P S Y L Y I I H K Q A P P L E D I A D D C S P G M R E L I E A S L E R N P N H R P R A A D L L K H E A L N P P R E D Q P R
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application FuncS | Reactivity Reacts | Dilution info - | Notes - |
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Recombinant human MAP3K8/COT protein is a Human Fragment protein, in the 30 to 397 aa range, expressed in Baculovirus infected Sf9, with >75% purity and suitable for SDS-PAGE, FuncS.
Alternative names=Mitogen-activated protein kinase kinase kinase 8, Cancer Osaka thyroid oncogene, Proto-oncogene c-Cot, Serine/threonine-protein kinase cot, Tumor progression locus 2, TPL-2, ESTF, COT, MAP3K8
Key facts
- Purity
>75% Densitometry
- Expression system
Baculovirus infected Sf9 cells
- Tags
GST tag N-Terminus
- Applications
SDS-PAGE, FuncS
- Biologically active
Yes
- Biological activity
Specific activity: 1012 nmol/min/mg
- Predicted molecular weight
70 kDa
- Amino acids
30 to 397
- Sequence
M E N L Y A S E E P A V Y E P S L M T M C Q D S N Q N D E R S K S L L L S G Q E V P W L S S V R Y G T V E D L L A F A N H I S N T A K H F Y G Q R P Q E S G I L L N M V I T P Q N G R Y Q I D S D V L L I P W K L T Y R N I G S D F I P R G A F G K V Y L A Q D I K T K K R M A C K L I P V D Q F K P S D V E I Q A C F R H E N I A E L Y G A V L W G E T V H L F M E A G E G G S V L E K L E S C G P M R E F E I I W V T K H V L K G L D F L H S K K V I H H D I K P S N I V F M S T K A V L V D F G L S V Q M T E D V Y F P K D L R G T E I Y M S P E V I L C R G H S T K A D I Y S L G A T L I H M Q T G T P P W V K R Y P R S A Y P S Y L Y I I H K Q A P P L E D I A D D C S P G M R E L I E A S L E R N P N H R P R A A D L L K H E A L N P P R E D Q P R
- Accession
- P41279-1
- Protein length
Fragment
- Animal free
No
- Nature
Recombinant
- Species
Human
- Concentration
- Storage buffer
pH: 7.5
Constituents: 25% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, 0.79% Tris HCl, 0.00385% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.0038% EGTA, 0.00292% EDTA, 0.00174% PMSF
Specifications
- Form
Liquid
- Additional notes
Affinity purified.
General info
- Function
Required for lipopolysaccharide (LPS)-induced, TLR4-mediated activation of the MAPK/ERK pathway in macrophages, thus being critical for production of the proinflammatory cytokine TNF-alpha (TNF) during immune responses. Involved in the regulation of T-helper cell differentiation and IFNG expression in T-cells. Involved in mediating host resistance to bacterial infection through negative regulation of type I interferon (IFN) production. In vitro, activates MAPK/ERK pathway in response to IL1 in an IRAK1-independent manner, leading to up-regulation of IL8 and CCL4. Transduces CD40 and TNFRSF1A signals that activate ERK in B-cells and macrophages, and thus may play a role in the regulation of immunoglobulin production. May also play a role in the transduction of TNF signals that activate JNK and NF-kappa-B in some cell types. In adipocytes, activates MAPK/ERK pathway in an IKBKB-dependent manner in response to IL1B and TNF, but not insulin, leading to induction of lipolysis. Plays a role in the cell cycle. Isoform 1 shows some transforming activity, although it is much weaker than that of the activated oncogenic variant.
- Sequence similarities
Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase kinase subfamily.
- Post-translational modifications
Autophosphorylated (PubMed:8226782, PubMed:1833717). Isoform 1 undergoes phosphorylation mainly on Ser residues, and isoform 2 on both Ser and Thr residues (PubMed:8226782). Phosphorylated on Thr-290; the phosphorylation is necessary but not sufficient for full kinase activity in vitro and for the dissociation of isoform 1 from NFKB1, leading to its degradation (PubMed:15466476, PubMed:15699325). Phosphorylated on Ser-400 by IKBKB; the phosphorylation is required for LPS-stimulated activation of the MAPK/ERK pathway in macrophages (PubMed:17472361, PubMed:22988300).
Storage
- Shipped at conditions
Dry Ice
- Appropriate long-term storage conditions
-80°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Avoid freeze / thaw cycle
This product is an active protein and may elicit a biological response in vivo, handle with caution.
Notes
ab43623 (Human ERK1 full length protein) and ab43614 (Human Myelin Basic Protein full length protein) can be utilized as a substrate for assessing kinase activity
This product was previously labelled as MAP3K8
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