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AB125554

Recombinant human MAP3K9 protein

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Recombinant human MAP3K9 protein is a Human Fragment protein, in the 1 to 433 aa range, expressed in Baculovirus infected Sf9 cells, with >85%, suitable for SDS-PAGE, WB, FuncS.

View Alternative Names

MLK1, PRKE1, MAP3K9, Mitogen-activated protein kinase kinase kinase 9, Mixed lineage kinase 1

4 Images
Functional Studies - Recombinant human MAP3K9 protein (AB125554)
  • FuncS

Unknown

Functional Studies - Recombinant human MAP3K9 protein (AB125554)

The specific activity of MAP3K9 (ab125554) was determined to be 133 nmol/min/mg as per activity assay protocol

SDS-PAGE - Recombinant human MAP3K9 protein (AB125554)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant human MAP3K9 protein (AB125554)

SDS PAGE analysis of ab125554

SDS-PAGE - Recombinant human MAP3K9 protein (AB125554)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant human MAP3K9 protein (AB125554)

SDS-PAGE analysis of ab125554.

Functional Studies - Recombinant human MAP3K9 protein (AB125554)
  • FuncS

Unknown

Functional Studies - Recombinant human MAP3K9 protein (AB125554)

The specific activity of ab125554 was determined to be 116 nmol/min/mg by Kinase Assay.

Key facts

Purity

>85% Densitometry

Expression system

Baculovirus infected Sf9 cells

Tags

Tag free

Applications

WB, SDS-PAGE, FuncS

applications

Biologically active

Yes

Biological activity

The specific activity of ab125554 was determined to be 116 nmol/min/mg.

Accession

P80192

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 7.5 Constituents: 25% Glycerol (glycerin, glycerine), 0.88% Sodium chloride, 0.79% Tris HCl, 0.31% Glutathione, 0.004% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.003% EDTA, 0.002% PMSF

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "FuncS": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

ab64311 (Myelin Basic Protein protein) can be utilized as a substrate for assessing kinase activity
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Sequence info

[{"sequence":"","proteinLength":"Fragment","predictedMolecularWeight":"77 kDa","actualMolecularWeight":null,"aminoAcidEnd":433,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":null,"accessionNumber":"P80192","tags":[]}]
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Properties and storage information

Shipped at conditions
Dry Ice
Appropriate short-term storage conditions
-80°C
Appropriate long-term storage conditions
-80°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
True
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Specifications

Form

Liquid

Additional notes

Purity was determined to be >85% by densitometry.

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General info

Function

Serine/threonine kinase which acts as an essential component of the MAP kinase signal transduction pathway. Plays an important role in the cascades of cellular responses evoked by changes in the environment. Once activated, acts as an upstream activator of the MKK/JNK signal transduction cascade through the phosphorylation of MAP2K4/MKK4 and MAP2K7/MKK7 which in turn activate the JNKs. The MKK/JNK signaling pathway regulates stress response via activator protein-1 (JUN) and GATA4 transcription factors. Also plays a role in mitochondrial death signaling pathway, including the release cytochrome c, leading to apoptosis.

Sequence similarities

Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase kinase subfamily.

Post-translational modifications

Autophosphorylation on serine and threonine residues within the activation loop plays a role in enzyme activation. Thr-312 is likely to be the main autophosphorylation site. Autophosphorylation also occurs on Thr-304 and Ser-308.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Serine/threonine kinase which acts as an essential component of the MAP kinase signal transduction pathway. Plays an important role in the cascades of cellular responses evoked by changes in the environment. Once activated, acts as an upstream activator of the MKK/JNK signal transduction cascade through the phosphorylation of MAP2K4/MKK4 and MAP2K7/MKK7 which in turn activate the JNKs. The MKK/JNK signaling pathway regulates stress response via activator protein-1 (JUN) and GATA4 transcription factors. Also plays a role in mitochondrial death signaling pathway, including the release cytochrome c, leading to apoptosis.
See full target information MAP3K9
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions
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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com