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Recombinant human MAP4K1/HPK1 protein (Active) is a Human Full Length protein, in the 1 to 833 aa range, expressed in Baculovirus infected Sf9, with >70% purity and suitable for SDS-PAGE, FuncS.

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Images

Functional Studies - Recombinant human MAP4K1/HPK1 protein (Active) (AB268641), expandable thumbnail
  • SDS-PAGE - Recombinant human MAP4K1/HPK1 protein (Active) (AB268641), expandable thumbnail

Key facts

Purity

>70% SDS-PAGE

Expression system

Baculovirus infected Sf9 cells

Tags

GST tag N-Terminus

Applications

SDS-PAGE, FuncS

Biologically active

Yes

Amino acid sequence

M D V V D P D I F N R D P R D H Y D L L Q R L G G G T Y G E V F K A R D K V S G D L V A L K M V K M E P D D D V S T L Q K E I L I L K T C R H A N I V A Y H G S Y L W L Q K L W I C M E F C G A G S L Q D I Y Q V T G S L S E L Q I S Y V C R E V L Q G L A Y L H S Q K K I H R D I K G A N I L I N D A G E V R L A D F G I S A Q I G A T L A R R L S F I G T P Y W M A P E V A A V A L K G G Y N E L C D I W S L G I T A I E L A E L Q P P L F D V H P L R V L F L M T K S G Y Q P P R L K E K G K W S A A F H N F I K V T L T K S P K K R P S A T K M L S H Q L V S Q P G L N R G L I L D L L D K L K N P G K G P S I G D I E D E E P E L P P A I P R R I R S T H R S S S L G I P D A D C C R R H M E F R K L R G M E T R P P A N T A R L Q P P R D L R S S S P R K Q L S E S S D D D Y D D V D I P T P A E D T P P P L P P K P K F R S P S D E G P G S M G D D G Q L S P G V L V R C A S G P P P N S P R P G P P P S T S S P H L T A H S E P S L W N P P S R E L D K P P L L P P K K E K M K R K G C A L L V K L F N G C P L R I H S T A A W T H P S T K D Q H L L L G A E E G I F I L N R N D Q E A T L E M L F P S R T T W V Y S I N N V L M S L S G K T P H L Y S H S I L G L L E R K E T R A G N P I A H I S P H R L L A R K N M V S T K I Q D T K G C R A C C V A E G A S S G G P F L C G A L E T S V V L L Q W Y Q P M N K F L L V R Q V L F P L P T P L S V F A L L T G P G S E L P A V C I G V S P G R P G K S V L F H T V R F G A L S C W L G E M S T E H R G P V Q V T Q V E E D M V M V L M D G S V K L V T P E G S P V R G L R T P E I P M T E A V E A V A M V G G Q L Q A F W K H G V Q V W A L G S D Q L L Q E L R D P T L T F R L L G S P R L E C S G T I S P H C N L L L P G S S N S P A S A S R V A G I T G L

Reactivity data

Application

SDS-PAGE

Reactivity

Reacts

Dilution info

-

Notes

-

Application

FuncS

Reactivity

Reacts

Dilution info

-

Notes

-

Target data

Function

Serine/threonine-protein kinase, which may play a role in the response to environmental stress (PubMed:24362026). Appears to act upstream of the JUN N-terminal pathway (PubMed:8824585). May play a role in hematopoietic lineage decisions and growth regulation (PubMed:8824585, PubMed:24362026). Able to autophosphorylate (PubMed:8824585). Together with CLNK, it enhances CD3-triggered activation of T-cells and subsequent IL2 production (By similarity).

Alternative names

Recommended products

Recombinant human MAP4K1/HPK1 protein (Active) is a Human Full Length protein, in the 1 to 833 aa range, expressed in Baculovirus infected Sf9, with >70% purity and suitable for SDS-PAGE, FuncS.

Alternative names

Key facts

Purity

>70% SDS-PAGE

Expression system

Baculovirus infected Sf9 cells

Applications

SDS-PAGE, FuncS

Biological activity

The specific activity of ab268641 as determined to 54 nmol/min/mg in a kinase assay using MBP substrate.

Accession
Q92918-1
Animal free

No

Species

Human

Concentration
Loading...
Storage buffer

pH: 7.5
Constituents: 25% Glycerol (glycerin, glycerine), 1.74% Sodium chloride, 0.79% Tris HCl, 0.31% Glutathione, 0.004% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.003% EDTA, 0.002% PMSF

Sequence info

Amino acid sequence

M D V V D P D I F N R D P R D H Y D L L Q R L G G G T Y G E V F K A R D K V S G D L V A L K M V K M E P D D D V S T L Q K E I L I L K T C R H A N I V A Y H G S Y L W L Q K L W I C M E F C G A G S L Q D I Y Q V T G S L S E L Q I S Y V C R E V L Q G L A Y L H S Q K K I H R D I K G A N I L I N D A G E V R L A D F G I S A Q I G A T L A R R L S F I G T P Y W M A P E V A A V A L K G G Y N E L C D I W S L G I T A I E L A E L Q P P L F D V H P L R V L F L M T K S G Y Q P P R L K E K G K W S A A F H N F I K V T L T K S P K K R P S A T K M L S H Q L V S Q P G L N R G L I L D L L D K L K N P G K G P S I G D I E D E E P E L P P A I P R R I R S T H R S S S L G I P D A D C C R R H M E F R K L R G M E T R P P A N T A R L Q P P R D L R S S S P R K Q L S E S S D D D Y D D V D I P T P A E D T P P P L P P K P K F R S P S D E G P G S M G D D G Q L S P G V L V R C A S G P P P N S P R P G P P P S T S S P H L T A H S E P S L W N P P S R E L D K P P L L P P K K E K M K R K G C A L L V K L F N G C P L R I H S T A A W T H P S T K D Q H L L L G A E E G I F I L N R N D Q E A T L E M L F P S R T T W V Y S I N N V L M S L S G K T P H L Y S H S I L G L L E R K E T R A G N P I A H I S P H R L L A R K N M V S T K I Q D T K G C R A C C V A E G A S S G G P F L C G A L E T S V V L L Q W Y Q P M N K F L L V R Q V L F P L P T P L S V F A L L T G P G S E L P A V C I G V S P G R P G K S V L F H T V R F G A L S C W L G E M S T E H R G P V Q V T Q V E E D M V M V L M D G S V K L V T P E G S P V R G L R T P E I P M T E A V E A V A M V G G Q L Q A F W K H G V Q V W A L G S D Q L L Q E L R D P T L T F R L L G S P R L E C S G T I S P H C N L L L P G S S N S P A S A S R V A G I T G L

Accession

Q92918

Protein length

Full Length

Amino acids

1 to 833

Nature

Recombinant

Tags

GST tag N-Terminus

Specifications

Form

Liquid

Additional notes

Affinity purified.

General info

Function

Serine/threonine-protein kinase, which may play a role in the response to environmental stress (PubMed:24362026). Appears to act upstream of the JUN N-terminal pathway (PubMed:8824585). May play a role in hematopoietic lineage decisions and growth regulation (PubMed:8824585, PubMed:24362026). Able to autophosphorylate (PubMed:8824585). Together with CLNK, it enhances CD3-triggered activation of T-cells and subsequent IL2 production (By similarity).

Sequence similarities

Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily.

Post-translational modifications

Autophosphorylates: phosphorylation promotes ubiquitination by the Cul7-RING(FBXW8) ubiquitin-protein ligase complex, leading to its degradation by the proteasome.

Storage

Shipped at conditions

Dry Ice

Appropriate long-term storage conditions

-80°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

This product is an active protein and may elicit a biological response in vivo, handle with caution.

Product promise

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2 product images

  • Functional Studies - Recombinant human MAP4K1/HPK1 protein (Active) (ab268641), expandable thumbnail

    Functional Studies - Recombinant human MAP4K1/HPK1 protein (Active) (ab268641)

    The specific activity of ab268641 as determined to 54 nmol/min/mg in a kinase assay using MBP substrate.

  • SDS-PAGE - Recombinant human MAP4K1/HPK1 protein (Active) (ab268641), expandable thumbnail

    SDS-PAGE - Recombinant human MAP4K1/HPK1 protein (Active) (ab268641)

    SDS-PAGE analysis of ab268641.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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