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AB177265

Recombinant human MAP4K3/GLK protein (Tagged)

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Recombinant human MAP4K3/GLK protein (Tagged) is a Human Fragment protein, in the 1 to 380 aa range, expressed in Baculovirus infected Sf9 cells, with >70%, suitable for SDS-PAGE, FuncS.

View Alternative Names

RAB8IPL1, MAP4K3, Mitogen-activated protein kinase kinase kinase kinase 3, Germinal center kinase-related protein kinase, MAPK/ERK kinase kinase kinase 3, GLK, MEK kinase kinase 3, MEKKK 3

4 Images
Functional Studies - Recombinant human MAP4K3/GLK protein (Tagged) (AB177265)
  • FuncS

Unknown

Functional Studies - Recombinant human MAP4K3/GLK protein (Tagged) (AB177265)

The specific activity of MAP4K3/GLK (ab177265) was determined to be 39.1 nmol/min/mg as per activity assay protocol and was equivalent to 91.9 nmol/min/mg as per radiometric assay

Functional Studies - Recombinant human MAP4K3/GLK protein (Tagged) (AB177265)
  • FuncS

Supplier Data

Functional Studies - Recombinant human MAP4K3/GLK protein (Tagged) (AB177265)

The specific activity of ab177265 was determined to be 92 nmol/min/mg.

SDS-PAGE - Recombinant human MAP4K3/GLK protein (Tagged) (AB177265)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant human MAP4K3/GLK protein (Tagged) (AB177265)

SDS-PAGE analysis of ab177265.

SDS-PAGE - Recombinant human MAP4K3/GLK protein (Tagged) (AB177265)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant human MAP4K3/GLK protein (Tagged) (AB177265)

SDS PAGE analysis of ab177265

Key facts

Purity

>70% Densitometry

Expression system

Baculovirus infected Sf9 cells

Tags

GST tag N-Terminus

Applications

FuncS, SDS-PAGE

applications

Biologically active

Yes

Biological activity

92 nmol/min/mg

Accession

Q8IVH8

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 7.5 Constituents: 25% Glycerol (glycerin, glycerine), 0.88% Sodium chloride, 0.79% Tris HCl, 0.31% Glutathione, 0.005% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.003% EDTA, 0.002% PMSF

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "FuncS": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

ab204848 (c Abl peptide) can be utilized as a substrate for assessing Kinase activity

Previously labelled as MAP4K3.

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Sequence info

[{"sequence":"MNPGFDLSRRNPQEDFELIQRIGSGTYGDVYKARNVNTGELAAIKVIKLEPGEDFAVVQQEIIMMKDCKHPNIVAYFGSYLRRDKLWICMEFCGGGSLQDIYHVTGPLSELQIAYVSRETLQGLYYLHSKGKMHRDIKGANILLTDNGHVKLADFGVSAQITATIAKRKSFIGTPYWMAPEVAAVERKGGYNQLCDLWAVGITAIELAELQPPMFDLHPMRALFLMTKSNFQPPKLKDKMKWSNSFHHFVKMALTKNPKKRPTAEKLLQHPFVTQHLTRSLAIELLDKVNNPDHSTYHDFDDDDPEPLVAVPHRIHSTSRNVREEKTRSEITFGQVKFDPPLRKETEPHHELPDSDGFLDSSEEIYYTARSNLDLQLEYG","proteinLength":"Fragment","predictedMolecularWeight":"70 kDa","actualMolecularWeight":null,"aminoAcidEnd":380,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Baculovirus infected Sf9 cells","accessionNumber":"Q8IVH8","tags":[{"tag":"GST","terminus":"N-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Dry Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-80°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
True
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Specifications

Form

Liquid

Additional notes

Affinity purified.

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General info

Function

Serine/threonine kinase that plays a role in the response to environmental stress. Appears to act upstream of the JUN N-terminal pathway (PubMed : 9275185). Activator of the Hippo signaling pathway which plays a pivotal role in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. MAP4Ks act in parallel to and are partially redundant with STK3/MST2 and STK4/MST2 in the phosphorylation and activation of LATS1/2, and establish MAP4Ks as components of the expanded Hippo pathway (PubMed : 26437443).

Sequence similarities

Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily.

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Product protocols

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Target data

Serine/threonine kinase that plays a role in the response to environmental stress. Appears to act upstream of the JUN N-terminal pathway (PubMed : 9275185). Activator of the Hippo signaling pathway which plays a pivotal role in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. MAP4Ks act in parallel to and are partially redundant with STK3/MST2 and STK4/MST2 in the phosphorylation and activation of LATS1/2, and establish MAP4Ks as components of the expanded Hippo pathway (PubMed : 26437443).
See full target information MAP4K3
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Complementary Products

Primary Antibodies

AB228752

Anti-MAP3K9 antibody

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Biochemicals

AB273391

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Chemical Structure - Linsitinib (OSI-906) (AB273391)

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Cell Lines & Lysates

AB258956

Human MAP4K3 (GLK) knockout HeLa cell lysate

Western blot - Human MAP4K3 (GLK) knockout HeLa cell lysate (AB258956)

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Cell Lines & Lysates

AB264796

Human MAP4K3 (GLK) knockout HeLa cell line

Advanced Validation

Western blot - Human MAP4K3 (GLK) knockout HeLa cell line (AB264796)

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