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AB101775

Recombinant human MAP4K4/NIK protein

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Recombinant human MAP4K4/NIK protein is a Human Fragment protein, in the 1 to 328 aa range, expressed in Baculovirus, with >95%, suitable for SDS-PAGE, WB, FuncS.

View Alternative Names

HGK, KIAA0687, NIK, MAP4K4, Mitogen-activated protein kinase kinase kinase kinase 4, HPK/GCK-like kinase HGK, MAPK/ERK kinase kinase kinase 4, Nck-interacting kinase, MEK kinase kinase 4, MEKKK 4

4 Images
Functional Studies - Recombinant human MAP4K4/NIK protein (AB101775)
  • FuncS

Unknown

Functional Studies - Recombinant human MAP4K4/NIK protein (AB101775)

The specific activity of MAP4K4/NIK (ab101775) was determined to be 61 nmol/min/mg as per activity assay protocol

Functional Studies - Recombinant human MAP4K4/NIK protein (AB101775)
  • FuncS

Unknown

Functional Studies - Recombinant human MAP4K4/NIK protein (AB101775)

The specific activity of ab101775 was determined to be 54 nmol/min/mg.

SDS-PAGE - Recombinant human MAP4K4/NIK protein (AB101775)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant human MAP4K4/NIK protein (AB101775)

SDS-PAGE analysis of ab101775.

SDS-PAGE - Recombinant human MAP4K4/NIK protein (AB101775)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant human MAP4K4/NIK protein (AB101775)

SDS PAGE analysis of ab101775

Key facts

Purity

>95% SDS-PAGE

Expression system

Baculovirus

Tags

Tag free

Applications

WB, FuncS, SDS-PAGE

applications

Biologically active

Yes

Biological activity

The specific activity of ab101775 was determined to be 54 nmol/min/mg.

Accession

O95819

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 7.5 Constituents: 25% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, 0.79% Tris HCl, 0.307% Glutathione, 0.00385% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.00292% EDTA, 0.00174% PMSF

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "FuncS": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

ab64311 (Myelin Basic Protein protein) can be utilized as a substrate for assessing kinase activity

This product was previously labelled as MAP4K4

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Sequence info

[{"sequence":"","proteinLength":"Fragment","predictedMolecularWeight":"64 kDa","actualMolecularWeight":null,"aminoAcidEnd":328,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":null,"accessionNumber":"O95819","tags":[]}]
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Properties and storage information

Shipped at conditions
Dry Ice
Appropriate short-term storage conditions
-80°C
Appropriate long-term storage conditions
-80°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
True
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Specifications

Form

Liquid

Additional notes

Purity was determined to be >95% by densitometry. Affinity purified.

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General info

Function

Serine/threonine kinase that plays a role in the response to environmental stress and cytokines such as TNF-alpha. Appears to act upstream of the JUN N-terminal pathway (PubMed : 9890973). Activator of the Hippo signaling pathway which plays a pivotal role in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. MAP4Ks act in parallel to and are partially redundant with STK3/MST2 and STK4/MST2 in the phosphorylation and activation of LATS1/2, and establish MAP4Ks as components of the expanded Hippo pathway (PubMed : 26437443). Phosphorylates SMAD1 on Thr-322 (PubMed : 21690388).

Sequence similarities

Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily.

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Product protocols

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Target data

Serine/threonine kinase that plays a role in the response to environmental stress and cytokines such as TNF-alpha. Appears to act upstream of the JUN N-terminal pathway (PubMed : 9890973). Activator of the Hippo signaling pathway which plays a pivotal role in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. MAP4Ks act in parallel to and are partially redundant with STK3/MST2 and STK4/MST2 in the phosphorylation and activation of LATS1/2, and establish MAP4Ks as components of the expanded Hippo pathway (PubMed : 26437443). Phosphorylates SMAD1 on Thr-322 (PubMed : 21690388).
See full target information MAP4K4
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